LOCUS       Exported                3694 bp ds-DNA     circular SYN 16-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Apr 19, 2019 from SnapGene Viewer 4.2.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..457)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     599..615
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        625..643
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    724..972
                     /label=U6 promoter
     misc_feature    973..1007
                     /label=AsCas12a-full_length-Direct-Repeat-crRNA
     promoter        complement(1067..1085)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1106..1122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1130..1146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1154..1184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1199..1220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1508..2096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2316..2418
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2419..3078
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3566..3671)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cactacgtga accatcaccc
      241 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
      301 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
      361 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      421 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
      481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      601 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg
      661 ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga
      721 tccgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
      781 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
      841 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
      901 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
      961 ggacgaaaca ccgtcaaaag acctttttaa tttctactct tgtagatttg tcttcgaaga
     1021 caattttttt gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     1081 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1141 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1201 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1261 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1321 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1381 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1441 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1501 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1561 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1621 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1681 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1741 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1801 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1861 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1921 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     1981 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2041 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2101 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2161 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttcgac cgaataaata
     2221 cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg
     2281 aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac
     2341 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttgtc gagattttca
     2401 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     2461 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     2521 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     2581 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattacgt
     2641 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     2701 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     2761 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     2821 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     2881 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     2941 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     3001 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     3061 tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggttgc
     3121 tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgaaagc aaattcgacc
     3181 cggtcgtcgg ttcagggcag ggtcgttaaa tagccgctta tgtctattgc tggtttacaa
     3241 gtttattgac taccggaagc agtgtgaccg tgtgcttctc aaatgcctga ggccagtttg
     3301 ctcaggctct ccccgtggag gtaataattg acgatatgat cctttttttc tgatcaaaag
     3361 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3421 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3481 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3541 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3601 aagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3661 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
