LOCUS       Exported                3695 bp ds-DNA     circular SYN 19-APR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   locusliu
  TITLE     Direct Submission
  JOURNAL   Exported Apr 19, 2019 from SnapGene Viewer 4.2.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..457)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     599..615
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        625..643
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    724..973
                     /label=U6 promoter
     misc_feature    974..1008
                     /label=LbCas12a-full_length-Direct-Repeat-crRNA
     promoter        complement(1068..1086)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1107..1123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1131..1147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1155..1185)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1200..1221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1509..2097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2317..2419
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2420..3079
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3567..3672)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cactacgtga accatcaccc
      241 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
      301 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
      361 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      421 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
      481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      601 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg
      661 ccccccctcg aggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga
      721 tccgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
      781 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
      841 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
      901 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
      961 ggacgaaaca ccgtttcaaa gattaaataa tttctactaa gtgtagattt gtcttcgaag
     1021 acaatttttt tgcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     1081 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     1141 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1201 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1261 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1321 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1381 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1441 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1501 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1561 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1621 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1681 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1741 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1801 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1861 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1921 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     1981 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2041 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2101 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2161 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttcga ccgaataaat
     2221 acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg
     2281 gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
     2341 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttgt cgagattttc
     2401 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     2461 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     2521 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     2581 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattacg
     2641 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     2701 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     2761 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     2821 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     2881 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     2941 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     3001 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     3061 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg
     3121 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac
     3181 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttaca
     3241 agtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt
     3301 gctcaggctc tccccgtgga ggtaataatt gacgatatga tccttttttt ctgatcaaaa
     3361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3601 caagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3661 aggggttccg cgcacatttc cccgaaaagt gccac
//

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