LOCUS       Exported               10649 bp ds-DNA     circular SYN 06-OCT-2015
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 23, 2019 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_structure  2028..2151
                     /label=cPPT
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    2028..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
                     /note="color: #ffe4c4"
     promoter        2209..3380
                     /label=EF-1a
                     /note="color: #66cc99; direction: RIGHT"
     intron          3168..3371
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
                     /note="color: #------"
     primer_bind     3364..3406
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     catttcaggtgtcgtgaggatccACCatgacaaatgatgtcac"
     primer_bind     3381..3408
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: 
                     cccatcgctagcatggatccACCatgacaaatgatgtcacct"
     misc_feature    3381
                     /label=MCS
                     /note="color: #00ccff"
     enhancer        3386
                     /label=MCS
                     /note="MCS"
                     /note="color: #800080"
     misc_feature    3390..3671
                     /label=HDHB-short
                     /note="HDHB-short"
                     /note="color: #0080ff; direction: RIGHT"
     CDS             3696..4409
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 3696 .. 3698 / #e1ff2f / 1a
                        2: 3699 .. 4409 / #e1ff2f"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     gene            3696..4409
                     /label=mVenus
                     /note="mVenus"
                     /note="color: #00e600; direction: RIGHT"
     primer_bind     3696..3713
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: 
                     ggttccggagccacgaacttctctctgttaaagcaagcaggagacgtggaagaaaatcc
                     cggtcctGTGAGCAAGGGCGAGGAG"
     primer_bind     complement(4387..4427)
                     /label=Fragment 1.REV
                     /note="color: black; sequence: 
                     gttcgtggctccggaacccttgtacagctcgtccatgccga"
     primer_bind     4410..4493
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: 
                     ggttccggagccacgaacttctctctgttaaagcaagcaggagacgtggaagaaaatcc
                     cggtcctGTGAGCAAGGGCGAGGAG"
     CDS             4419..4475
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 4472"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4476..5180
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     intron          5181..5204
                     /label=Linker
                     /note="Linker"
                     /note="color: #ff8000"
     gene            5205..5252
                     /label=NLS
                     /note="NLS"
                     /note="color: #00e600; direction: RIGHT"
     intron          5256..5303
                     /label=NES
                     /note="NES"
                     /note="color: #ff8000"
     primer_bind     5297..5343
                     /label=Primer 2
                     /note="color: black; sequence: 
                     GAGATCAAGAATCTTGAGCACTAGTGATGGAAGTAAACATCTCCCAG"
     primer_bind     complement(5302..5343)
                     /label=Primer 1
                     /note="color: black; sequence: 
                     CTGGGAGATGTTTACTTCCATCACTAGTGCTCAAGATTCTTG"
     misc_feature    5322..5453
                     /label=CDK4 Docking Site (Rb Cterm)
                     /note="CDK4 Docking Site (Rb Cterm)"
                     /note="color: #0080ff"
     primer_bind     complement(5426..5473)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     cggccgccctcgaggaattcTTACTTCTCTTCCTTGTTTGAGGTATCC"
     primer_bind     complement(5426..5459)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     ggctgattatgatcagttatctagaattcTTACTTCTCTTCCTTGTTTGAGGTATCC"
     polyA_signal    5459
                     /note="color: #a6acb3"
     misc_feature    5459
                     /label=MCS
                     /note="color: #00ccff"
     misc_feature    5460..6118
                     /label=IRES
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    5504..6115
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             6119..6547
                     /codon_start=1
                     /label=BlastR
                     /note="color: #ff9966"
                     /translation="MVMKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEI
                     ISAVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGM
                     CRELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     misc_feature    6571..7162
                     /label=WPRE
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    6571..7159
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7042..7053)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7041"
                     /translation="IEGR"
     LTR             7367..8000
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(8129..8145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    8153..8169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(8177..8207)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 8177 .. 8183 / #ffffff / -10
                        2: 8184 .. 8201 / #ffffff
                        3: 8202 .. 8207 / #ffffff / -35"
     protein_bind    8222..8243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(8531..9116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(9287..10147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  9287 .. 10078 / #ccffcc
                        2: 10079 .. 10147 / #ccffcc / signal sequence
                     Cleavage site after base 10078"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10148..10252)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    10300..10434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccacca tgacaaatga tgtcacctgg agcgaggcct
     3421 cttcgcctga tgagaggaca ctcacctttg ctgaaagatg gcaattatct tcacctgatg
     3481 gagtagatac agatgatgat ttaccaaaat cgcgagcatc caaaagaacc tgtggtgtga
     3541 atgatgatga aagtccaagc aaaattttta tggtgggaga atctccacaa gtgtcttcca
     3601 gacttcagaa tttgagactg aataatttaa ttcccaggca acttttcaag cccaccgata
     3661 atcaagaaac tggatccggg gcccagggca gcggcgtgag caagggcgag gagctgttca
     3721 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3781 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca
     3841 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc
     3901 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3961 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4021 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4081 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4141 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     4201 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4261 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca
     4321 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4381 tcactctcgg catggacgag ctgtacaagg gttccggagc cacgaacttc tctctgttaa
     4441 agcaagcagg agacgtggaa gaaaatcccg gtcctgtgag caagggcgag gaggataaca
     4501 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     4561 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     4621 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     4681 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     4741 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     4801 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     4861 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     4921 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     4981 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     5041 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     5101 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     5161 gcatggacga gctgtacaag agtgctggtg gtagtgctgg tggtaagcgc tcggccggtc
     5221 tcaacgaaga cgagtcgcca tctaagaagc tgccatcgcc gaccgtgtcg tcaaggctgg
     5281 agcgcctgac cctccagaga tcaagaatct tgagcactag tgatggaagt aaacatctcc
     5341 caggagagtc caaatttcag cagaaactgg cagaaatgac ttctactcga acacgaatgc
     5401 aaaagcagaa aatgaatgat agcatggata cctcaaacaa ggaagagaag taagaattcc
     5461 tcgagggcgg ccgctctaga gtcgacgggc cgcggtaaca attgttaact aacttaagct
     5521 agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga
     5581 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     5641 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     5701 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     5761 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     5821 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5881 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5941 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     6001 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     6061 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat
     6121 ggtcatgaaa acatttaaca tttctcaaca agatctagaa ttagtagaag tagcgacaga
     6181 gaagattaca atgctttatg aggataataa acatcatgtg ggagcggcaa ttcgtacgaa
     6241 aacaggagaa atcatttcgg cagtacatat tgaagcgtat ataggacgag taactgtttg
     6301 tgcagaagcc attgcgattg gtagtgcagt ttcgaatgga caaaaggatt ttgacacgat
     6361 tgtagctgtt agacaccctt attctgacga agtagataga agtattcgag tggtaagtcc
     6421 ttgtggtatg tgtagggagt tgatttcaga ctatgcacca gattgttttg tgttaataga
     6481 aatgaatggc aagttagtca aaactacgat tgaagaactc attccactca aatatacccg
     6541 aaattaaccc gggcggggcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     6601 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6661 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6721 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6781 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6841 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6901 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6961 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7021 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7081 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7141 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     7201 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     7261 aggaggagga ggtgggtttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     7321 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     7381 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7441 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7501 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7561 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     7621 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     7681 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     7741 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     7801 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7861 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7921 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7981 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8041 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     8101 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8161 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8221 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8281 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8341 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8401 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8461 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8521 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8581 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8641 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8701 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8761 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8821 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8881 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8941 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9001 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9061 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9181 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9241 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9421 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9781 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10141 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10261 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat
    10321 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10381 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    10441 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    10501 aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    10561 aagaaattgt atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt
    10621 tgttaaatat gtactacaaa cttagtagt
//
