LOCUS       Exported                8310 bp ds-DNA     circular SYN 19-SEP-2017
DEFINITION  TALENs were constructed using EZ-TAL TALE Assembly Kit (System 
            Bioscience), according to manufacturer's instructions, and the 
            resulting SFTPC TALENS encoding plasmids were named EF1a-TALEN_NN 
            (SPC left) and EF1a-TALEN_HD (SPC right), respectively.TALENs were 
            designed to target the sequences close to the translation initiation
            (ATG) site of the human SFTPC gene. All TALENs and donor template 
            plasmid maps and sequences are available at www.kottonlab.com. The 
            SFTPC TALEN recognition sequences are: left TALEN 5'-TAG CAC CTG CAG
            CAA GAT GG-3' and right TALEN 5'-TCA CCG GCG GGC TCT CCA TC-3'. 
            Between the two binding sites is a 22 bp spacer (ATG TGG GCA GCA AAG
            AGG TCC T).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Apr 22, 2019 from SnapGene Viewer 4.3.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..806
                     /label=Ori
     promoter        join(1464..1860,1862,1864..2009)
                     /label=EF1 promoter
     misc_feature    2040..2159
                     /label=NLS
     misc_feature    2160..2567
                     /label=truncated N-term
     misc_feature    2568..4464
                     /label=SPC Right
     misc_feature    4465..4524
                     /label=0.5 repeat HD
     misc_feature    4525..4713
                     /label=TAL C-term
     misc_feature    4714..5316
                     /label=FokI
     misc_feature    5554..5697
                     /label=SV40 ori
     misc_feature    5943..6968
                     /label=hygromycin resistance
     misc_feature    7096..7226
                     /label=SV40 polyadenylation signal
     misc_feature    7281..8129
                     /label=Amp resistance
ORIGIN
        1 ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc
       61 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
      121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      241 tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      841 gcgaggaagc ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc
      901 tgacggatgg cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt
      961 cttaagctcg ggccccctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg
     1021 taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag
     1081 aatatttaag ggcgcctgtc actttgcttg atatatgaga attatttaac cttataaatg
     1141 agaaaaaagc aacgcacttt aaataagata cgttgctttt tcgattgatg aacacctata
     1201 attaaactat tcatctatta tttatgattt tttgtatata caatatttct agtttgttaa
     1261 agagaattaa gaaaataaat ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa
     1321 attatacatg tcaacgataa tacaaaatat aatacaaact ataagatgtt atcagtattt
     1381 attatcattt agaataaatt ttgtgtcgcc cttaattgtg agcggataac aattacgagc
     1441 ttcatgcaca gtggcgttgc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     1501 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     1561 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     1621 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     1681 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     1741 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     1801 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     1861 ggtccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     1921 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     1981 tacagatcca agctgtgacc ggcgcctacg ctagaatacg actcactata ggggccacca
     2041 tggactataa ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg
     2101 acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
     2161 tagatttgag aactttggga tattcacagc agcagcagga aaagatcaag cccaaagtga
     2221 ggtcgacagt cgcgcagcat cacgaagcgc tggtgggtca tgggtttaca catgcccaca
     2281 tcgtagcctt gtcgcagcac cctgcagccc ttggcacggt cgccgtcaag taccaggaca
     2341 tgattgcggc gttgccggaa gccacacatg aggcgatcgt cggtgtgggg aaacagtgga
     2401 gcggagcccg agcgcttgag gccctgttga cggtcgcggg agagctgaga gggcctcccc
     2461 ttcagctgga cacgggccag ttgctgaaga tcgcgaagcg gggaggagtc acggcggtcg
     2521 aggcggtgca cgcgtggcgc aatgcgctca cgggagcacc cctcaacctg accccagagc
     2581 aggtcgtggc aattgcgagc catgacgggg gaaagcaggc actcgaaacc gtccagaggt
     2641 tgctgcctgt gctgtgccaa gcgcacggac ttacgccaga gcaggtcgtg gcaattgcga
     2701 gcaacatcgg gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc
     2761 aagcgcacgg actaacccca gagcaggtcg tggcaattgc gagccatgac gggggaaagc
     2821 aggcactcga aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac gggttgaccc
     2881 cagagcaggt cgtggcaatt gcgagccatg acgggggaaa gcaggcactc gaaaccgtcc
     2941 agaggttgct gcctgtgctg tgccaagcgc acggcctgac cccagagcag gtcgtggcaa
     3001 ttgcgagcaa caacggggga aagcaggcac tcgaaaccgt ccagaggttg ctgcctgtgc
     3061 tgtgccaagc gcacggactg acaccagagc aggtcgtggc aattgcgagc aacaacgggg
     3121 gaaagcaggc actcgaaacc gtccagaggt tgctgcctgt gctgtgccaa gcgcacggac
     3181 ttacacccga acaagtcgtg gcaattgcga gccatgacgg gggaaagcag gcactcgaaa
     3241 ccgtccagag gttgctgcct gtgctgtgcc aagcgcacgg acttacgcca gagcaggtcg
     3301 tggcaattgc gagcaacaac gggggaaagc aggcactcga aaccgtccag aggttgctgc
     3361 ctgtgctgtg ccaagcgcac ggactaaccc gagagcaggt cgtggcaatt gcgagcaaca
     3421 acgggggaaa gcaggcactc gaaaccgtcc agaggttgct gcctgtgctg tgccaagcgc
     3481 acgggttgac cccagagcag gtcgtggcaa ttgcgagcaa caacggggga aagcaggcac
     3541 tcgaaaccgt ccagaggttg ctgcctgtgc tgtgccaagc gcacggcctg accccagagc
     3601 aggtcgtggc aattgcgagc catgacgggg gaaagcaggc actcgaaacc gtccagaggt
     3661 tgctgcctgt gctgtgccaa gcgcacggac tgacaccaga gcaggtcgtg gcaattgcga
     3721 gcaacggagg gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc
     3781 aagcgcacgg cctcacccca gagcaggtcg tggcaattgc gagccatgac gggggaaagc
     3841 aggcactcga aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac ggacttacgc
     3901 cagagcaggt cgtggcaatt gcgagcaacg gagggggaaa gcaggcactc gaaaccgtcc
     3961 agaggttgct gcctgtgctg tgccaagcgc acggactaac cccagagcag gtcgtggcaa
     4021 ttgcgagcca tgacggggga aagcaggcac tcgaaaccgt ccagaggttg ctgcctgtgc
     4081 tgtgccaagc gcacgggttg accccagagc aggtcgtggc aattgcgagc catgacgggg
     4141 gaaagcaggc actcgaaacc gtccagaggt tgctgcctgt gctgtgccaa gcgcacggcc
     4201 tgaccccaga gcaggtcgtg gcaattgcga gcaacatcgg gggaaagcag gcactcgaaa
     4261 ccgtccagag gttgctgcct gtgctgtgcc aagcgcacgg actgacacca gagcaggtcg
     4321 tggcaattgc gagcaacgga gggggaaagc aggcactcga aaccgtccag aggttgctgc
     4381 ctgtgctgtg ccaagcgcac ggactcacgc ctgagcaggt agtggctatt gcatcccatg
     4441 acgggggcag acccgcactg gagtctcacg cctgagcagg tagtggctat tgcatcccat
     4501 gacgggggca gacccgcact ggagtcaatc gtggcccagc tttcgaggcc ggaccccgcg
     4561 ctggccgcac tcactaatga tcatcttgta gcgctggcct gcctcggcgg acgacccgcc
     4621 ttggatgcgg tgaagaaggg gctcccgcac gcgcctgcat tgattaagcg gaccaacaga
     4681 aggattcccg agaggacatc acatcgagtg gcaggttccc aactcgtgaa gagtgaactt
     4741 gaggagaaaa agtcggagct gcggcacaaa ttgaaatacg taccgcatga atacatcgaa
     4801 cttatcgaaa ttgctaggaa ctcgactcaa gacagaatcc ttgagatgaa ggtaatggag
     4861 ttctttatga aggtttatgg ataccgaggg aagcatctcg gtggatcacg aaaacccgac
     4921 ggagcaatct atacggtggg gagcccgatt gattacggag tgatcgtcga cacgaaagcc
     4981 tacagcggtg ggtacaatct tcccatcggg caggcagatg agatgcaacg ttatgtcgaa
     5041 gaaaatcaga ccaggaacaa acacatcaat ccaaatgagt ggtggaaagt gtatccttca
     5101 tcagtgaccg agtttaagtt tttgtttgtc tctgggcatt tcaaaggcaa ctataaggcc
     5161 cagctcacac ggttgaatca cattacgaac tgcaatggtg cggttttgtc cgtagaggaa
     5221 ctgctcattg gtggagaaat gatcaaagcg ggaactctga cactggaaga agtcagacgc
     5281 aagtttaaca atggcgagat caatttccgc tcataaaaaa tcagcctcga ctgtgccttc
     5341 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5401 cactcccact gtcctttcct aataaaatga ggaaattgca tcacaacact caaccctatc
     5461 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5521 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     5581 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5641 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5701 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5821 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5881 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     5941 tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     6001 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     6061 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     6121 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     6181 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     6241 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     6301 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     6361 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     6421 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     6481 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     6541 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     6601 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
     6661 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
     6721 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
     6781 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
     6841 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
     6901 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
     6961 aggaatagca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7021 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7081 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7201 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7261 aatcatggtc attaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7321 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7381 ccatctggcc ccagcgctgc gatgataccg cgagaaccac gctcaccggc tccggattta
     7441 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7501 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7561 agtttgcgca acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7621 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7681 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7741 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7801 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7861 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7921 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7981 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8041 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8101 ataagggcga cacggaaatg ttgaatactc atattcttcc tttttcaata ttattgaagc
     8161 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8221 caaatagggg tcagtgttac aaccaattaa ccaattctga acattatcgc gagcccattt
     8281 atacctgaat atggctcata acaccccttg
//
