LOCUS       PL_07_I8\(pRS413U-P1_TRX2-5xUAS-ALD6)        7664 bp    DNA
            circular
DEFINITION  pRS413_USER
ACCESSION   pL_02_I6\(pRS413U-P1_TRX2_v1-ALD6)
VERSION     pL_02_I6\(pRS413U-P1_TRX2_v1-ALD6).0
KEYWORDS    .

COMMENT
DISPLAY_SETUP_START|
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                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="9277978" name="f1_origin" shape="1"
                target="f1_origin:1861 - 1555" type="33" xsi:type="symbol">
                <style name="Shape 3" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="51" green="51" red="153"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="102" green="153" red="255"/>
                    </fill>
                </style>
                <location x="80" y="40"/>
                <dimension height="350" width="350"/>
            </element>
            <element id="1360768397" target="1360768397" text="@L bp"
                type="-2055714368" xsi:type="label">
                <style name="Molecule Label 2" xsi:type="basicStyle">
                    <label text="@L bp">
                        <font name="Arial" size="18" style="0"/>
                        <color blue="50" green="50" red="50"/>
                    </label>
                </style>
                <location x="225" y="219"/>
            </element>
            <element id="250185998" target="250185998" text="@N"
                type="346519525" xsi:type="label">
                <style name="Molecule Label 1" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="24" style="1"/>
                        <color blue="50" green="50" red="50"/>
                    </label>
                </style>
                <location x="5" y="191"/>
            </element>
            <element id="2037688856" name="pTRX2"
                target="pTRX2:4109 - 3835" text="@N" type="29" xsi:type="label">
                <style name="Signal Label" xsi:type="basicStyle">
                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="207" y="413"/>
            </element>
            <element id="2037688856" name="pTRX2"
                target="pTRX2:4109 - 3835" type="29" xsi:type="line">
                <style name="Signal Label" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
                    </line>
                </style>
            </element>
            <element id="2037688856" name="pTRX2" shape="1"
                target="pTRX2:4109 - 3835" type="29" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="55" y="15"/>
                <dimension height="400" width="400"/>
            </element>
        </elements>
    </setupInfo>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
        prefer1LetterAACode="false" preferSingleStrand="false"
        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="sequenceSetup">
        <textRangeSetupList/>
    </setupInfo>
    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="AvaI|cycgrg,EcoRI|gaattc,SmaI|cccggg,PstI|ctgcag,ApaLI|gtgcac,NcoI|ccatgg,HindIII|aagctt,BamHI|ggatcc,XmaI|cccggg,ClaI|atcgat"
        enzymesCleavePoint="AvaI|2,EcoRI|2,SmaI|4,PstI|6,ApaLI|2,NcoI|2,HindIII|2,BamHI|2,XmaI|2,ClaI|3"
        enzymesComplCleavePoint="AvaI|0,EcoRI|0,SmaI|0,PstI|0,ApaLI|0,NcoI|0,HindIII|0,BamHI|0,XmaI|0,ClaI|0"
        enzymesPalindromic="AvaI|true,EcoRI|true,SmaI|true,PstI|true,ApaLI|true,NcoI|true,HindIII|true,BamHI|true,XmaI|true,ClaI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
    <setupInfo complete="true" completeMinSize="150"
        completeNested="false" includeStopCodon="false"
        incomplete="false" incompleteMinSize="30" selected="false"
        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
    <setupInfo analysisSubsets="Analysis Results (MAIN)" selected="true" type="Auto Load Analysis"/>
</vntiDisplaySetup>
DISPLAY_SETUP_END|
COMMENT     ORIGDB|GenBank
            This file is created by Vector NTI
            http://www.lifetechnologies.com/
            VNTAUTHORNAME|Demo User
            VNTOAUTHORNAME|UNKNOWN
FEATURES             Location/Qualifiers
     misc_feature    complement(29..51)
                     /label=pGEX_3_primer
                     /label=pGEX_3_primer
                     /vntifkey="21"
                     /original_location="complement(29..51)"
     CDS             504..1163
                     /label=HIS3
                     /label=HIS3
                     /vntifkey="4"
                     /original_location="504..1163"
     rep_origin      complement(1555..1861)
                     /label=f1_origin
                     /label=f1_origin
                     /vntifkey="33"
                     /original_location="complement(1555..1861)"
     misc_feature    complement(1894..2037)
                     /label=lacZ_a
                     /label=lacZ_a
                     /vntifkey="21"
                     /original_location="complement(1894..2037)"
     CDS             complement(6090..6950)
                     /label=AmpR
                     /label=AmpR
                     /vntifkey="4"
                     /original_location="complement(3709..4569)"
     promoter        complement(6992..7020)
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /vntifkey="30"
                     /original_location="complement(4611..4639)"
     misc_feature    7082..7600
                     /label=CEN6_ARS4
                     /label=CEN6_ARS4
                     /vntifkey="21"
                     /original_location="4701..5219"
     terminator      complement(2096..2290)
                     /label=T ADH1
                     /label=T ADH1
                     /vntifkey="43"
                     /original_location="complement(2096..2290)"
     terminator      4701..4871
                     /label=T CYC1
                     /label=T CYC1
                     /vntifkey="43"
                     /original_location="2320..2490"
     enhancer        complement(3820..3825)
                     /label=Kozak
                     /label=Kozak
                     /vntifkey="9"
                     /original_location="3130..3135"
     CDS             complement(2317..3819)
                     /label=ALD6
                     /label=ALD6
                     /vntifkey="4"
     misc_feature    complement(3835..4687)
                     /label=pTRX2_5xUAS
                     /type="misc_feature"
                     /vntifkey="21"
     enhancer        complement(4408..4547)
                     /label=TRX2_UAS
                     /vntifkey="9"
     enhancer        complement(4259..4398)
                     /label=TRX2_UAS
                     /vntifkey="9"
     enhancer        complement(4548..4687)
                     /label=TRX2_UAS
                     /vntifkey="9"
     enhancer        complement(4119..4258)
                     /label=TRX2_UAS
                     /vntifkey="9"
     eukaryotic      complement(3835..4109)
                     /label=pTRX2
                     /original_location="790..1064"
                     /promoter_type="eukaryotic"
                     /type="eukaryotic"
                     /vntifkey="29"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgcgtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgagagcg
     2101 acctcatgct atacctgaga aagcaacctg acctacagga aagagttact caagaataag
     2161 aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat tttttttata
     2221 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtgtcaaca
     2281 acgtatctac caacggaatg cgtgcgatcg tgcgatttac aacttaattc tgacagcttt
     2341 tacttcagtg tatgcatggt agacttcttc acccatttct ctaccgtaac cagattgctt
     2401 aacaccaccg aatggaactc tggagtcaaa atcgttgtat gtgttgatcc agacggtacc
     2461 ggccttcaac atcttggcca ccttcaaacc tgtgctcaaa gattctgttt cgataccaga
     2521 acctagaccg aattcagagc tgttagccat ttcgacacct tcttctaaag tcttgaactt
     2581 tgcgacagtg acaactggtc caaaaatttc ttccttaaca attctcatgt cttcattaac
     2641 atcgtagaaa acggttggtc tgatgaagta acccttgtca ccaacttttt cgccaccagt
     2701 taagatcttg gcgccttctt tcttaccgat atcgatgtag ttcataattg tgtcgaattg
     2761 ttgacggtta gtgatagcac cttggaagtt agccttgtca aatggattac caactttgat
     2821 ttcggtttcc aagtaagcct tgaaagcagc caatagttcg tcgtaaatac cttcttgaac
     2881 gtaaattcta gaaccagagg aacaaatttg accagcgttc ttgaaaatac cgtttactag
     2941 atttggtaaa gtcttcttaa tgttagcatc gtcaaagacc aaatgggcgg acttaccacc
     3001 tagttccaaa gtgattttct tcaagttaga ttcagaagag tcgacagcaa cactcttacc
     3061 gacttctgta gaaccggtaa aagccagctt tctgattctt gggtcgttgg tcaaagcagc
     3121 accaacagtt ctaccaggac ctggaacgat gttgacgaca ccagctggaa taccaacctt
     3181 cttacataaa gaagcaaagt atagggcatt taaaggtgtg acagcagcgg gtttcaagat
     3241 acagacgtta cccatggcca atgctggggc gatcttccaa gccaacatca ttattggaaa
     3301 gttccatgga ataatttgac cacagacacc gattggctct aaggtggtga agttcatgta
     3361 gccgtcaccg gtgttgattg ttctaccgtt gactttgtcg gcataggcag cagcatctct
     3421 tagacagttg attgcaatgg taacatcccc acgggctaag gccaaagttt taccattgtc
     3481 caaagcttca atggaagaaa ccaagtcaat ttggctttcc aattcgtcag ccaacttact
     3541 tagtagacgg cctctttctc ttgggtcttg ggtagcccat tcagtgtcgt ggaaagcacg
     3601 gtcggcacat tcgatagcat attcaacatc ttcagtggtg gcagaagaga cctcacaaac
     3661 ggtgttttca gtggaaggat cttcgacggg ataggtctta ccgtcttgag ctttcataaa
     3721 cttgttgtta atgaatagac cggttggttg ctcgtatgtc aaaccatttg gaagtgtgat
     3781 cttgactggt tcagcagtgt caaagtgtag cttagtcatt gttttacctg cacttattga
     3841 tgtgttattt aaagatatcg tagactctcg tgtatgtgtg cgtgtataat tcttgctaga
     3901 caacaaataa aaactggtcc tcgttattct cttgtcagct tttcatcccc cgaatggctt
     3961 tcttatatac tgatatccct ctttcggctg gctggaaact gaacgcgctt agtaagaaaa
     4021 gagccacctt gtagggagca tcctttacta agagtataaa caattacaca tatactttga
     4081 tgattactta atattcactg gcactcaaga tgacagatct gatatccctc tttcggctgg
     4141 ctggaaactg aacgcgctta gtaagaaaag agccaccttg tagggagcat cctttactaa
     4201 gagtataaac aattacacat atactttgat gattacttaa tattcactgg cactcaagct
     4261 gatatccctc tttcggctgg ctggaaactg aacgcgctta gtaagaaaag agccaccttg
     4321 tagggagcat cctttactaa gagtataaac aattacacat atactttgat gattacttaa
     4381 tattcactgg cactcaagag tgcaggtctg atatccctct ttcggctggc tggaaactga
     4441 acgcgcttag taagaaaaga gccaccttgt agggagcatc ctttactaag agtataaaca
     4501 attacacata tactttgatg attacttaat attcactggc actcaagctg atatccctct
     4561 ttcggctggc tggaaactga acgcgcttag taagaaaaga gccaccttgt agggagcatc
     4621 ctttactaag agtataaaca attacacata tactttgatg attacttaat attcactggc
     4681 actcaagatc gcgtgcattc atccgctcta accgaaaagg aaggagttag acaacctgaa
     4741 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt
     4801 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc
     4861 ttgcttgaga agagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     4921 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4981 ataggagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca
     5041 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5101 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5161 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5221 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5761 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6001 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6061 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6121 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6181 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6241 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6301 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6361 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6421 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6481 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6541 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6601 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6661 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6721 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6781 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6841 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6901 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6961 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7021 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7081 gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta atataaatat
     7141 ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga
     7201 agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg
     7261 ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa
     7321 tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt
     7381 cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt
     7441 tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa
     7501 atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa
     7561 gatacgaggc gcgtgtaagt tacaggcaag cgatccgtcc taagaaacca ttattatcat
     7621 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
