LOCUS       pICSL4723-Pi21_guides_ 17449 bp ds-DNA     circular     24-APR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             3068..3859
                     /label="NPTII"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(9068..9153)
                     /label="Porcine 2A"
                     /ApEinfo_revcolor=#8811ff
                     /ApEinfo_fwdcolor=#8811ff
     misc_RNA        15722..15797
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    15339..15701
                     /label="TaU6"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     conflict        121..139
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4757..4781
                     /label="Actual LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4688..4838)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(5137..6232)
                     /label="KanR\(NptII)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     intron          complement(5427..5730)
                     /label="UBQ10-i1\intron"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    16226..16245
                     /label="gRNA38"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(7674..7897)
                     /label="NosTerm"
                     /ApEinfo_revcolor=#5b7c23
                     /ApEinfo_fwdcolor=#5b7c23
     primer_bind     100..119
                     /label="RBf1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(9154..13493)
                     /label="WH Cas9"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    17274..17293
                     /label="gRNA39"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(8832..9068)
                     /label="Histine 2A Targetting"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    15863..16225
                     /label="TaU6"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Promoter        complement(6237..7649)
                     /label="Act1 (Oryza sativa)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    complement(17381..17405)
                     /label="attB2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    16911..17273
                     /label="TaU6"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(5133..5136)
                     /label="GCTT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3068..3859
                     /label="Taken from pBIN19"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(5427..5428)
                     /label="3'\splice\site\AG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_binding    complement(6233..6236)
                     /label="AATG\overhang"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_RNA        16770..16845
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(14..139)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    complement(16857..16881)
                     /label="attB2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     variation       4180..4180
                     /label="C to T"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(13494..15313)
                     /label="Ubi Promoter"
                     /ApEinfo_revcolor=#0813ff
                     /ApEinfo_fwdcolor=#0813ff
     misc_feature    15702..15721
                     /label="gRNA36"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_RNA        17294..17369
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Terminator      complement(4870..5132)
                     /label="nost"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4688..4838
                     /label="Taken from pBIN19"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(8779..8831)
                     /label="GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_RNA        16246..16321
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     variation       32..56
                     /label="Actual RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     splicing_signal complement(5730..5730)
                     /label="5'\splice\site\GT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    175..2955
                     /label="pVS1 fragment short"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     4771..4791
                     /label="L1f"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3837..3859
                     /label="New Feature"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4022..4636
                     /label="rep - pMB1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    complement(16333..16357)
                     /label="attB2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_difference complement(5627..5627)
                     /label="G\to\T"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    16387..16749
                     /label="TaU6"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    11..139
                     /label="Taken from pBIN19"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(7899..8777)
                     /label="GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Golden_Gate_Par complement(6237..7653)
                     /label="Pro+5U_OsAct1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     variation       3550..3550
                     /label="A to G (BpiI removed)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(4866..4869)
                     /label="CGCT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    complement(15809..15833)
                     /label="attB2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    16750..16769
                     /label="gRNA37"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     variation       3749..3749
                     /label="A to C, I to L"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4621..4621)
                     /label="ORI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
       61 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
      121 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtca gccgtgcggc
      181 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
      241 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
      301 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
      361 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
      421 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
      481 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
      541 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
      601 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
      661 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
      721 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
      781 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
      841 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
      901 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
      961 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     1021 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     1081 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     1141 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     1201 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     1261 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     1321 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
     1381 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat
     1441 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
     1501 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
     1561 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
     1621 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
     1681 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
     1741 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
     1801 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
     1861 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
     1921 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
     1981 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
     2041 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
     2101 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
     2161 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
     2221 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
     2281 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
     2341 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
     2401 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
     2461 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
     2521 ttgccctagc aggggaaaaa ggtcgaaaaa gcttctttcc tgtggatagc acgtacattg
     2581 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
     2641 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
     2701 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
     2761 ctggccagcg cacagccgaa cagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc
     2821 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc
     2881 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc
     2941 gcccacatca aggctccgag tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3001 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3061 gaagagtatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
     3121 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
     3181 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
     3241 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
     3301 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
     3361 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
     3421 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
     3481 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
     3541 ctgggaagag gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
     3601 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
     3661 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
     3721 gtatgacatt gccttctgcg tccggtcgct cagggaggat atcggggaag aacagtatgt
     3781 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
     3841 tttactggat gaattgtttt agctgtcaga ccaagtttac tcatatatac tttagattga
     3901 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3961 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4021 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4081 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4141 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4201 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4261 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4321 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4381 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4441 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4501 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4561 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4621 aaacgccagc aacgcggcct ttttacggtt cctgctcgga tctgttggac cggacagtag
     4681 tcatggttga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
     4741 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
     4801 ttaataacac attgcggacg tttttaatgt actggggttg aacactctgt gccgaattcg
     4861 gatccAGCGT CGATCTAGTA ACATAGATGA CACCGCGCGC GATAATTTAT CCTAGTTTGC
     4921 GCGCTATATT TTGTTTTCTA TCGCGTATTA AATGTATAAT TGCGGGACTC TAATCATAAA
     4981 AACCCATCTC ATAAATAACG TCATGCATTA CATGTTAATT ATTACATGCT TAACGTAATT
     5041 CAACAGAAAT TATATGATAA TCATCGCAAG ACCGGCAACA GGATTCAATC TTAAGAAACT
     5101 TTATTGCCAA ATGTTTGAAC GATCTGCTTG ACAAGCtcag aagaactcgt caagaaggcg
     5161 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     5221 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     5281 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     5341 catgatattc ggcaagcagg catcgccgtg ggtcacgacg agatcctcgc cgtcgggcat
     5401 ccgcgccttg agcctggcga acagttctgt taatcagaaa aactcggatt aatcgacaaa
     5461 ttcgatcgca caaactagaa actaacacca gatctagata gaaatcacaa gtcgaagagt
     5521 aattattcga caaaactcaa attatttgaa caaatcggat gatatctatg aaaccctaat
     5581 cgagaattaa gatgatatct aacgatcaaa cccagaaaat cgtcttagat ctaagattaa
     5641 cagaatctaa accaaagaac atatacgaaa ttgggatcga acgaaaacaa aatcgaagat
     5701 tttgagagaa taaggaacac agaaacttac ctgcaggcgc gagcccctga tgctcttcgt
     5761 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     5821 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     5881 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     5941 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     6001 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttggagtt
     6061 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     6121 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     6181 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcattGGCT
     6241 TCTACCTACA AAAAAGCTCC GCACGAGGCT GCATTTGTCA CAAATCATGA AAAGAAAAAC
     6301 TACCGATGAA CAATGCTGAG GGATTCAAAT TCTACCCACA AAAAGAAGAA AGAAAGATCT
     6361 AGCACATCTA AGCCTGACGA AGCAGCAGAA ATATATAAAA ATATAAACCA TAGTGCCCTT
     6421 TTCCCCTCTT CCTGATCTTG TTTAGCATGG CGGAAATTTT AAACCCCCCA TCATCTCCCC
     6481 CAACAACGGC GGATCGCAGA TCTACATCCG AGAGCCCCAT TCCCCGCGAG ATCCGGGCCG
     6541 GATCCACGCC GGCGAGAGCC CCAGCCGCGA GATCCCGCCC CTCCCGCGCA CCGATCTGGG
     6601 CGCGCACGAA GCCGCCTCTC GCCCACCCAA ACTACCAAGG CCAAAGATCG TGTCCGAGAC
     6661 GGAAAAAAAA AACGGAGAAA GAAAGAGGAG AGGGGCGGGG TGGTTACCGG CGCGGCGGCG
     6721 GCGGAGGGGG AGGGGGGAGG AGCTCGTCGT CCGGCAGCGA GGGGGGAGGA GGTGGAGGTG
     6781 GTGGTGGTGG TGGTGGTAGG GTTGGGGGGA TGGGAGGAGA GGGGGGGGTA TGTATATAGT
     6841 GGCGATGGGG GGCGTTTCTT TGGAAGCGGA GGGAGGGCCG GCCTCGTCGC TGGCTCGCGA
     6901 TCCTCCTCGC GTTTCCGGCC CCCACGACCC GGACCCACCT GCTGTTTTTT CTTTTTCTTT
     6961 TTTTTCTTTC TTTTTTTTTT TTTGGCTGCG AGACGTGCGG TGCGTGCGGA CAACTCACGG
     7021 TGATAGTGGG GGGGTGTGGA GACTATTGTC CAGTTGGCTG GACTGGGGTG GGTTGGGTTG
     7081 GGTTGGGTTG GGCTGGGCTT GCTATGGATC GTGGATAGCA CTTTGGGCTT TAGGAACTTT
     7141 AGGGGTTGTT TTTGTAAATG TTTTGAGTCT AAGTTTATCT TTTATTTTTA CTAGAAAAAA
     7201 TACCCATGCG CTGCAACGGG GGAAAGCTAT TTTAATCTTA TTATTGTTCA TTGTGAGAAT
     7261 TCGCCTGAAT ATATATTTTT CTCAAAAATT ATGTCAAATT AGCATATGGG TTTTTTTAAA
     7321 GATATTTCTT ATACAAATCC CTCTGTATTT ACAAAAGCAA ACGAACTTAA AACCCGACTC
     7381 AAATACAGAT ATGCATTTCC AAAAGCGAAT AAACTTAAAA ACCAATTCAT ACAAAAATGA
     7441 CGTATCAAAG TACCGACAAA AACATCCTCA ATTTTTATAA TAGTAGAAAA GAGTAAATTT
     7501 CACTTTGGGC CACCTTTTAT TACCGATATT TTACTTTATA CCACCTTTTA ACTGATGTTT
     7561 TCACTTTTGA CCAGGTAATC TTACCTTTGT TTTATTTTGG ACTATCCCGA CTCTCTTCTC
     7621 AAGCATATGA ATGACCTCGA GTATGCTAGC TCCgcaaGAA TTCAAGCTTA GCGTATATTT
     7681 TGTTTTCTAT CGCGTATTAA ATGTATAATT GCGGGACTCT AATCATAAAA ACCCATCTCA
     7741 TAAATAACGT CATGCATTAC ATGTTAATTA TTACATGCTT AACGTAATTC AACAGAAATT
     7801 ATATGATAAT CATCGCAAGA CCGGCAACAG GATTCAATCT TAAGAAACTT TATTGCCAAA
     7861 TGTTTGAACA CTAGTGATAT CTGCGGCCGC AGTTATCACT TGTAGAGCTC ATCCATCCCA
     7921 TGAGTGATGC CTGCAGCCGT TACAAACTCC AGAAGCACCA TGTGATCCCT CTTTTCGTTA
     7981 GGGTCCTTAG ACAGAACGGA TTGGGTTGAC AGGTAGTGAT TGTCGGGCAG AAGCACTGGG
     8041 CCATCGCCTA TCGGCGTGTT CTGCTGGTAG TGGTCAGCCA GTTGGACACT ACCATCCTCC
     8101 ACATTATGCC GGATTTTGAA GTTCGCCTTA ATGCCGTTCT TCTGCTTGTC GGCAGTAATG
     8161 TAGACGTTGT GAGAATTGAA GTTGTACTCG AGCTTATGCC CCAGTATGTT GCCGTCCTCT
     8221 TTGAAGTCGA TCCCTTTGAG TTCGATCCTG TTCACCAGTG TATCTCCCTC GAACTTGACC
     8281 TCCGCACGTG TCTTGTACGT TCCGTCGTCC TTAAAGCTGA TGGTGCGTTC TTGGACATAC
     8341 CCCTCAGGCA TGGCTGACTT GAAGAAGTCA TGGCGTTTCA TGTGGTCCGG ATACCTCGAA
     8401 AAGCACTGAA CACCATATGT GAGAGTGGTG ACCAACGTTG GCCATGGAAC CGGCAGTTTC
     8461 CCAGTGGTAC AGATGAACTT GAGGGTAAGC TTGCCATTGG TGGCATCACC TTCACCTTCG
     8521 CCTCTCACGC TGAACTTGTG GCCATTTACG TCGCCATCCA ACTCGACCAA AATGGGCACA
     8581 ACTCCTGTAA AGAGCTCCTC TCCTTTGGAC ACCATGAAAG AGGCGCCCTG AGCAGAACTC
     8641 GTAAACTTCG TAACCGCCTT AGTCCCTTCA GACACAGCAT GTTTCGCCAA CTCACCGGGC
     8701 AACACAAGTC TAACCGCAGT CTGAATCTCC CTGGAAGTAA TCGTCGGCTT CTTGTTGTAC
     8761 CTCGCCAGCT TCGAAGATTC ACCAGCGAGC TTCTCAAAAA TATCGTTGAT GAAACTGTTC
     8821 ATAATTCCCA TCGCTTTGCT TGAGATTCCG ATGTCTGGAT GAACTTGCTT CAACACCTTG
     8881 AAGATGTAGA TCTTGTATGT CTCAACGTTC TTCTTTGATC TCTTCTTCTT CTTGTCTCCG
     8941 GCGACGGCTG GTTCCTTGGG GAGTTTCTTT CCGGCTTTTG GTTTCTTCTC TGCCGCTGCT
     9001 GCTGGTTCGA CTGCCGGAGT TTTCTCTGCC GGTTTCTTCT CCGCTGGTTT CTTATCTGCC
     9061 TTCGCCATGG ACGTTGAGCC TGGAGCTGGG CCAGGATTCT CCTCCACATC ACCAGCTTGC
     9121 TTGAGCAGGG AAAAGTTCGT GGCTCCGGAT CCGCCGTCGA CTTTGCGCTT CTTCTTGGGA
     9181 TCCTCCACTT TCCGCTTCTT CTTAGGGGAA CCAGACCCAA GGGAGGCGTA ATCTGGCACG
     9241 TCATACGGAT AGGCATCTCC TCCTAGTTGC GACAAGTCGA TCCTCGTTTC GTACAGGCCT
     9301 GTAATAGACT GGTGAATTAG GGTGGCATCG AGTACTTCTT TGGTGCTGGT ATAGCGCTTC
     9361 CTATCAATCG TCGTGTCAAA ATATTTGAAC GCCGCAGGAG CGCCAAGGTT CGTCAACGTG
     9421 AACAAGTGTA TGATGTTTTC TGCCTGCTCC CGGATGGGTT TGTCCCGATG CTTGTTGTAG
     9481 GCGGACAACA CCTTGTCAAG GTTAGCGTCA GCAAGAATCA CCCTCTTCGA GAACTCACTG
     9541 ATCTGTTCGA TGATCTCATC GAGGTAGTGC TTGTGTTGCT CCACAAACAG CTGCTTTTGC
     9601 TCATTGTCCT CTGGGGAACC CTTAAGTTTC TCATAGTGTG AGGCGAGATA CAGGAAGTTG
     9661 ACGTACTTGG ATGGGAGTGC CAGCTCGTTC CCCTTTTGCA ACTCGCCTGC GCTGGCAAGC
     9721 ATCCTCTTTC TCCCATTCTC CAGCTCGAAC AAGCTGTATT TCGGCAACTT TATGATGAGA
     9781 TCTTTCTTGA CTTCCTTGTA TCCTTTCGCC TCCAAGAAAT CAATGGGATT CTTCTCAAAG
     9841 CTGGAACGCT CCATAATGGT AATCCCGAGC AACTCCTTTA CGCTCTTCAG CTTCTTCGAC
     9901 TTCCCCTTTT CCACCTTGGC AACAACCAGC ACGGAGTACG CCACTGTAGG ACTGTCAAAG
     9961 CCACCGTACT TCTTTGGATC CCAGTCTTTC TTACGGGCTA TAAGCTTATC GCTATTGCGC
    10021 TTCGGAAGGA TTGACTCTTT CGAGAAGCCG CCGGTTTGGA CTTCCGTTTT CTTGACGATG
    10081 TTGACTTGTG GCATGCTTAG CACCTTCCGG ACAGTAGCAA AGTCTCTCCC CTTGTCCCAC
    10141 ACTATCTCAC CAGTTTCTCC GTTAGTCTCT ATCAGCGGAC GTTTTCTGAT CTCTCCATTG
    10201 GCCAGGGTGA TCTCAGTCTT GAAGAAGTTC ATTATGTTGC TGTAAAAGAA GTACTTGGCG
    10261 GTGGCCTTGC CGATTTCCTG CTCACTCTTC GCAATCATCT TCCTTACGTC ATAAACCTTG
    10321 TAGTCACCGT AGACGAACTC CGACTCAAGC TTGGGATATT TCTTGATGAG TGCAGTGCCA
    10381 ACCACAGCAT TGAGATAGGC ATCATGGGCG TGATGGTAGT TGTTGATCTC ACGGACCTTA
    10441 TAGAACTGGA AGTCCTTCCG AAAGTCGGAC ACCAGTTTAG ATTTCAGAGT AATAACTTTG
    10501 ACTTCCCGGA TTAGCTTGTC ATTCTCATCG TATTTTGTGT TCATGCGTGA GTCCAGGATC
    10561 TGTGCAACGT GCTTGGTGAT TTGCCGTGTC TCAACCAGTT GGCGCTTGAT AAATCCGGCC
    10621 TTATCTAGCT CACTGAGGCC GCCTCTCTCC GCCTTGGTAA GGTTGTCGAA CTTCCTTTGT
    10681 GTAATGAGCT TTGCATTGAG GAGTTGCCTC CAGTAGTTCT TCATCTTCTT GACGACTTCC
    10741 TCAGAAGGCA CGTTGTCTGA CTTCCCGCGG TTCTTGTCGG ACCTGGTCAG CACCTTGTTG
    10801 TCGATAGAGT CGTCTTTGAG GAAGCTTTGT GGTACTATAT GATCGACATC GTAGTCAGAT
    10861 AGGCGATTGA TGTCGAGCTC CTGGTCCACG TACATATCGC GTCCGTTTTG GAGGTAGTAG
    10921 AGGTAGAGCT TCTCGTTTTG CAGTTGGGTG TTTTCGACCG GATGCTCCTT GAGAATTTGT
    10981 GATCCGAGCT CCTTGATGCC TTCTTCGATT CTCTTCATCC TTTCCCTGGA GTTCTTCTGC
    11041 CCCTTCTGTG TGGTTTGGTT CTCCCGAGCC ATCTCAATCA CGATATTCTC GGGCTTATGT
    11101 CTACCCATCA CTTTGACCAG CTCATCGACA ACCTTCACTG TTTGAAGTAT ACCCTTCTTG
    11161 ATGGCTGGAG ATCCGGCCAA ATTCGCGATA TGCTCGTGGA GCGAATCGCC TTGGCCTGAA
    11221 ACCTGAGCCT TCTGAATGTC CTCCTTGAAG GTCAAGGAGT CATCGTGGAT TAGCTGCATA
    11281 AAGTTCCTGT TTGCGAACCC ATCGGACTTG AGGAAGTCGA GGATGGTCTT GCCCGACTGC
    11341 TTATCGCGAA TGCCATTGAT CAGCTTCCTG CTCAGCCTGC CCCAACCTGT GTAGCGCCTG
    11401 CGCTTCAGCT GCTTCATCAC TTTGTCGTCA AAGAGGTGCG CATACGTCTT GAGCCTCTCT
    11461 TCGATCATCT CGCGGTCCTC GAAGAGAGTA AGCGTCAGAA CGATGTCCTC CAAGATGTCC
    11521 TCGTTCTCTT CGTTATCGAG GAAGTCTTTA TCCTTAATGA TCTTGAGCAA GTCGTGGTAC
    11581 GTTCCCAGCG AGGCGTTGAA GCGATCCTCA ACGCCGCTTA TCTCCACAGA GTCAAAGCAC
    11641 TCGATTTTCT TAAAGTAGTC CTCCTTTAGC TGCTTGACTG TGACCTTGCG ATTGGTCTTG
    11701 AAAAGGAGAT CCACTATGGC CTTCTTCTGC TCTCCTGACA GAAAGGCCGG TTTCCTCATA
    11761 CCCTCAGTAA CGTACTTGAC CTTGGTCAGC TCGTTGTAGA CGGTGAAGTA CTCGTACAGT
    11821 AGGGAATGTT TGGGGAGGAC CTTCTCGTTT GGCAGATTCT TGTCGAAGTT GGTCATCCTC
    11881 TCGATGAAGC TTTGGGCAGA AGCGCCCTTG TCCACCACCT CCTCGAAGTT CCATGGGGTA
    11941 ATCGTTTCCT CCGATTTTCT AGTCATCCAC GCGAACCTAG AGTTGCCCCT TGCCAATGGG
    12001 CCCACATAGT AGGGGATTCT GAATGTGAGG ATCTTCTCGA TTTTCTCCCG GTTGTCTTTG
    12061 AGGAATGGGT AAAAGTCCTC TTGCCTACGA AGTATGGCGT GAAGCTCGCC CAGATGGATT
    12121 TGGTGTGGAA TGCTTCCATT GTCGAAGGTA CGCTGCTTGC GGAGTAGATC CTCGCGATTG
    12181 AGCTTAACCA GGAGCTCTTC AGTGCCATCC ATCTTCTCGA GAATGGGCTT GATAAACTTG
    12241 TAGAACTCCT CCTGTGACGC GCCACCGTCG ATGTACCCAG CATACCCGTT CTTTGATTGA
    12301 TCGAAGAAGA TCTCTTTGTA CTTCTCCGGC AGCTGTTGTC TGACGAGAGC CTTGAGGAGC
    12361 GTGAGATCCT GGTGATGTTC GTCGTACCGT TTGATCATGG ATGCCGAAAG AGGAGCCTTG
    12421 GTAATCTCAG TGTTAACGCG CAGAATATCG GACAAGAGGA TTGCATCTGA CAAGTTCTTC
    12481 GCTGCAAGGA AAAGGTCCGC ATACTGGTCG CCGATTTGGG CAAGCAGATT GTCGAGATCG
    12541 TCGTCGTACG TGTCTTTGGA CAGTTGGAGC TTGGCATCCT CGGCCAGGTC AAAGTTAGAC
    12601 TTGAAGTTAG GGGTGAGCCC TAGACTAAGG GCAATAAGGT TGCCAAACAA TCCGTTCTTC
    12661 TTTTCGCCTG GCAGTTGGGC TATCAGATTT TCGAGCCTCC GGCTTTTCGA CAGCCGAGCA
    12721 CTCAAGATTG CCTTCGCATC AACTCCTGAC GCGTTGATGG GGTTCTCTTC AAAGAGCTGA
    12781 TTGTATGTCT GGACCAGTTG GATGAAGAGT TTGTCAACAT CGGAATTGTC CGGATTAAGG
    12841 TCGCCTTCGA TGAGGAAGTG CCCACGGAAC TTAATCATAT GGGCCAGGGC CAAATAGATG
    12901 AGGCGCAAAT CAGCCTTGTC AGTGCTATCT ACGAGCTTCT TCCTCAGGTG ATAGATGGTA
    12961 GGATACTTCT CGTGGTATGC GACTTCGTCG ACAATGTTCC CGAATATCGG ATGGCGTTCG
    13021 TGCTTCTTGT CCTCTTCTAC GAGGAAGCTT TCCTCGAGCC GATGGAAGAA GGAGTCATCC
    13081 ACTTTCGCCA TTTCGTTGGA AAAGATCTCT TGTAGGTAGC AGATCCTGTT CTTTCTACGC
    13141 GTATAGCGCC TTCTTGCTGT GCGCTTGAGG CGGGTCGCTT CGGCTGTCTC CCCGGAATCA
    13201 AAGAGCAACG CACCTATTAG GTTCTTCTTG ATGGAGTGCC TGTCGGTGTT GCCCAGCACT
    13261 TTAAATTTCT TCGATGGCAC CTTGTACTCG TCAGTGATGA CAGCCCAACC CACACTATTC
    13321 GTTCCTATAT CCAACCCGAT AGAGTACTTC TTGTCCATGG TGGCAATGCC AGGACCCCTG
    13381 TACCGAAGGC TGCTAATCCG ACACACCCTA CCCACCTTCC TCTTCTTCTT CGGCCCCATG
    13441 GTGGCACGCG TGAGAAATCT CGAGAGCTCC AGATTCTCCC CGGGAATTCC AGATCCTCTA
    13501 GAGTCGACCT GCAGAAGTAA CACCAAACAA CAGGGTGAGC ATCGACAAAA GAAACAGTAC
    13561 CAAGCAAATA AATAGCGTAT GAAGGCAGGG CTAAAAAAAA TCCACATATA GCTGCTGCAT
    13621 ATGCCATCAT CCAAGTATAT CAAGATCAAA ATAATTATAA AACATACTTG TTTATTATAA
    13681 TAGATAGGTA CTCAAGGTTA GAGCATATGA ATAGATGCTG CATATGCCAT CATGTATATG
    13741 CATCAGTAAA ACCCACATCA ACATGTATAC CTATCCTAGA TCGATATTTC CATCCATCTT
    13801 AAACTCGTAA CTATGAAGAT GTATGACACA CACATACAGT TCCAAAATTA ATAAATACAC
    13861 CAGGTAGTTT GAAACAGTAT TCTACTCCGA TCTAGAACGA ATGAACGACC GCCCAACCAC
    13921 ACCACATCAT CACAACCAAG ACAAAAAAAA GCATGAAAAG ATGACCCGAC AAACAAGTGC
    13981 ACGGCATATA TTGAAATAAA GGAAAAGGGC AAACCAAACC CTATGCAACG AAACAAAAAA
    14041 AATCATGAAA TCGATCCCGT CTGCGGAACG GCTAGAGCCA TCCCAGGATT CCCCAAAGAG
    14101 AAACACTGGC AAGTTAGCAA TCAGAACGTG TCTGACGTAC AGGTCGCATC CGTGTACGAA
    14161 CGCTAGCAGC ACGGATCTAA CACAAACACG GATCTAACAC AAACATGAAC AGAAGTAGAA
    14221 CTACCGGGCC CTAACCATGG ACCGGAACGC CGATCTAGAG AAGGTAGAGA GGGGGGGGGG
    14281 GGGAGGACGA GCGGCGTACC TTGAAGCGGA GGTGCCGACG GGTGGATTTG GGGGAGATCT
    14341 GGTTGTGTGT GTGTGCGCTC CGAACAACAC GAGGTTGGGG AAAGAGGGTG TGGAGGGGGT
    14401 GTCTATTTAT TACGGCGGGC GAGGAAGGGA AAGCGAAGGA GCGGTGGGAA AGGAATCCCC
    14461 CGTAGCTGCC GGTGCCGTGA GAGGAGGAGG AGGCCGCCTG CCGTGCCGGC TCACGTCTGC
    14521 CGCTCCGCCA CGCAATTTCT GGATGCCGAC AGCGGAGCAA GTCCAACGGT GGAGCGGAAC
    14581 TCTCGAGAGG GGTCCAGAGG CAGCGACAGA GATGCCGTGC CGTCTGCTTC GCTTGGCCCG
    14641 ACGCGACGCT GCTGGTTCGC TGGTTGGTGT CCGTTAGACT CGTCGACGGC GTTTAACAGG
    14701 CTGGCATTAT CTACTCGAAA CAAGAAAAAT GTTTCCTTAG TTTTTTTAAT TTCTTAAAGG
    14761 GTATTTGTTT AATTTTTAGT CACTTTATTT TATTCTATTT TATATCTAAA TTATTAAATA
    14821 AAAAAACTAA AATAGAGTTT TAGTTTTCTT AATTTAGAGG CTAAAATAGA ATAAAATAGA
    14881 TGTACTAAAA AAATTAGTCT ATAAAAACCA TTAACCCTAA ACCCTAAATG GATGTACTAA
    14941 TAAAATGGAT GAAGTATTAT ATAGGTGAAG CTATTTGCAA AAAAAAAGGA GAACACATGC
    15001 ACACTAAAAA GATAAAACTG TAGAGTCCTG TTGTCAAAAT ACTCAATTGT CCTTTAGACC
    15061 ATGTCTAACT GTTCATTTAT ATGATTCTCT AAAACACTGA TATTATTGTA GTACTATAGA
    15121 TTATATTATT CGTAGAGTAA AGTTTAAATA TATGTATAAA GATAGATAAA CTGCACTTCA
    15181 AACAAGTGTG ACAAAAAAAA TATGTGGTAA TTTTTTATAA CTTAGACATG CAATGCTCAT
    15241 TATCTCTAGA GAGGGGCACG ACCGGGTCAC GCTGCACTGC AGGCATGCAA GCTTCACCAC
    15301 TTTGTACCAT GGTGCTCCAC TAGAATTCGA GCTCGGAGGA CCAAGCCCGT TATTCTGACA
    15361 GTTCTGGTGC TCAACACATT TATATTTATC AAGGAGCACA TTGTTACTCA CTGCTAGGAG
    15421 GGAATCGAAC TAGGAATATT GATCAGAGGA ACTACGAGAG AGCTGAAGAT AACTGCCCTC
    15481 TAGCTCTCAC TGATCTGGGT CGCATAGTGA GATGCAGCCC ACGTGAGTTC AGCAACGGTC
    15541 TAGCGCTGGG CTTTTAGGCC CGCATGATCG GGCTTTTGTC GGGTGGTCGA CGTGTTCACG
    15601 ATTGGGGAGA GCAACGCAGC AGTTCCTCTT AGTTTAGTCC CACCTCGCCT GTCCAGCAGA
    15661 GTTCTGACCG GTTTATAAAC TCGCTTGCTG CATCAGACTT GAGATCGAGA TCGTAAAACA
    15721 Agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
    15781 tggcaccgag tcggtgcttt ttttctagac ccagctttct tGTACAAAGT TGGCATTACG
    15841 CTTTACGAAT TCCCATGGGG AGGACCAAGC CCGTTATTCT GACAGTTCTG GTGCTCAACA
    15901 CATTTATATT TATCAAGGAG CACATTGTTA CTCACTGCTA GGAGGGAATC GAACTAGGAA
    15961 TATTGATCAG AGGAACTACG AGAGAGCTGA AGATAACTGC CCTCTAGCTC TCACTGATCT
    16021 GGGTCGCATA GTGAGATGCA GCCCACGTGA GTTCAGCAAC GGTCTAGCGC TGGGCTTTTA
    16081 GGCCCGCATG ATCGGGCTTT TGTCGGGTGG TCGACGTGTT CACGATTGGG GAGAGCAACG
    16141 CAGCAGTTCC TCTTAGTTTA GTCCCACCTC GCCTGTCCAG CAGAGTTCTG ACCGGTTTAT
    16201 AAACTCGCTT GCTGCATCAG ACTTGTATGG CACGAACTTG TATGTgtttt agagctagaa
    16261 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
    16321 ctttttttct agacccagct ttcttGTACA AAGTTGGCAT TACGCTCAGA GAATTCGCAT
    16381 GCGGAGGACC AAGCCCGTTA TTCTGACAGT TCTGGTGCTC AACACATTTA TATTTATCAA
    16441 GGAGCACATT GTTACTCACT GCTAGGAGGG AATCGAACTA GGAATATTGA TCAGAGGAAC
    16501 TACGAGAGAG CTGAAGATAA CTGCCCTCTA GCTCTCACTG ATCTGGGTCG CATAGTGAGA
    16561 TGCAGCCCAC GTGAGTTCAG CAACGGTCTA GCGCTGGGCT TTTAGGCCCG CATGATCGGG
    16621 CTTTTGTCGG GTGGTCGACG TGTTCACGAT TGGGGAGAGC AACGCAGCAG TTCCTCTTAG
    16681 TTTAGTCCCA CCTCGCCTGT CCAGCAGAGT TCTGACCGGT TTATAAACTC GCTTGCTGCA
    16741 TCAGACTTGA TATCAAAATC GTACCACAAg ttttagagct agaaatagca agttaaaata
    16801 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttctagaccc
    16861 agctttcttG TACAAAGTTG GCATTACGCT TGTGGAATTC CTCGAGGGAG GACCAAGCCC
    16921 GTTATTCTGA CAGTTCTGGT GCTCAACACA TTTATATTTA TCAAGGAGCA CATTGTTACT
    16981 CACTGCTAGG AGGGAATCGA ACTAGGAATA TTGATCAGAG GAACTACGAG AGAGCTGAAG
    17041 ATAACTGCCC TCTAGCTCTC ACTGATCTGG GTCGCATAGT GAGATGCAGC CCACGTGAGT
    17101 TCAGCAACGG TCTAGCGCTG GGCTTTTAGG CCCGCATGAT CGGGCTTTTG TCGGGTGGTC
    17161 GACGTGTTCA CGATTGGGGA GAGCAACGCA GCAGTTCCTC TTAGTTTAGT CCCACCTCGC
    17221 CTGTCCAGCA GAGTTCTGAC CGGTTTATAA ACTCGCTTGC TGCATCAGAC TTGTAGGGCA
    17281 TGAACTTGTA TGTgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
    17341 aacttgaaaa agtggcaccg agtcggtgct ttttttctag acccagcttt cttGTACAAA
    17401 GTTGGCATTA CGCTGAGCtg agaccgagga tgcacatgtg accgaggga
//