LOCUS       Exported                4708 bp ds-DNA     circular SYN 25-APR-2019
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    pCfB8739 (pUC19-XdCrtYB->)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2019 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            271..2292
                     /label=XdCrtYB
                     /label=ORF1
                     /label=SsGGPS7
     primer_bind     complement(2312..2329)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2312..2328)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2321..2343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2537..2556)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2656..2678
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2716..2734)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2802..2906
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2907..3767
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     primer_bind     complement(3125..3144)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    3938..4526
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4427..4446
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4680..4697
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc atgacggctc tcgcatatta ccagatccat
      301 ctgatctata ctctcccaat tcttggtctt ctcggcctgc tcacttcccc gattttgaca
      361 aaatttgaca tctacaaaat atcgatcctc gtatttattg cgtttagtgc aaccacacca
      421 tgggactcat ggatcatcag aaatggcgca tggacatatc catcagcgga gagtggccaa
      481 ggcgtgtttg gaacgtttct agatgttcca tatgaagagt acgctttctt tgtcattcaa
      541 accgtaatca ccggcttggt ctacgtcttg gcaactaggc accttctccc atctctcgcg
      601 cttcccaaga ctagatcgtc cgccctttct ctcgcgctca aggcgctcat ccctctgccc
      661 attatctacc tatttaccgc tcaccccagc ccatcgcccg acccgctcgt gacagatcac
      721 tacttctaca tgcgggcact ctccttactc atcaccccac ctaccatgct cttggcagca
      781 ttatcaggcg aatatgcttt cgattggaaa agtggccgag caaagtcaac tattgcaaca
      841 atcatgatcc cgacggtgta tctgatttgg gtagattatg ttgctgtcgg tcaagactct
      901 tggtcgatca acgatgagaa gattgtaggg tggaggcttg gaggtgtact acccattgag
      961 gaagctatgt tcttcttact gacgaatcta atgattgttc tgggtctgtc tgcctgcgat
     1021 catactcagg ccctatacct gctacacggt cgaactattt atggcaacaa aaagatgcca
     1081 tcttcatttc ccctcattac accgcctgtg ctctccctgt tttttagcag ccgaccatac
     1141 tcttctcagc caaaacgtga cttggaactg gcagtcaagt tgttggagga aaagagccgg
     1201 agcttttttg ttgcctcggc tggatttcct agcgaagtta gggagaggct ggttggacta
     1261 tacgcattct gccgggtgac tgatgatctt atcgactctc ctgaagtatc ttccaacccg
     1321 catgccacaa ttgacatggt ctccgatttt cttaccctac tatttgggcc cccgctacac
     1381 ccttcgcaac ctgacaagat cctttcttcg cctttacttc ctccttcgca cccttcccga
     1441 cccacgggaa tgtatcccct cccgcctcct ccttcgctct cgcctgccga gctcgttcaa
     1501 ttccttaccg aaagggttcc cgttcaatac catttcgcct tcaggttgct cgctaagttg
     1561 caagggctga tccctcgata cccactcgac gaactcctta gaggatacac cactgatctt
     1621 atctttccct tatcgacaga ggcagtccag gctcggaaga cgcctatcga gaccacagct
     1681 gacttgctgg actatggtct atgtgtagca ggctcagtcg ccgagctatt ggtctatgtc
     1741 tcttgggcaa gtgcaccaag tcaggtccct gccaccatag aagaaagaga agctgtgtta
     1801 gtggcaagcc gagagatggg aactgccctt cagttggtga acattgctag ggacattaaa
     1861 ggggacgcaa cagaagggag attttaccta ccactctcat tctttggtct tcgggatgaa
     1921 tcaaagcttg cgatcccgac tgattggacg gaacctcggc ctcaagattt cgacaaactc
     1981 ctcagtctat ctccttcgtc cacattacca tcttcaaacg cctcagaaag cttccggttc
     2041 gaatggaaga cgtactcgct tccattagtc gcctacgcag aggatcttgc caaacattct
     2101 tataagggaa ttgaccgact tcctaccgag gttcaagcgg gaatgcgagc ggcttgcgcg
     2161 agctacctac tgatcggccg agagatcaaa gtcgtttgga aaggagacgt cggagagaga
     2221 aggacagttg ccggatggag gagagtacgg aaagtcttga gtgtggtcat gagcggatgg
     2281 gaagggcagt aagggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact
     2341 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2401 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2461 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2521 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     2581 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     2641 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     2701 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2761 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2821 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2881 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2941 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3001 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3061 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3121 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3181 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3241 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3301 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3361 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3421 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3481 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3541 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3601 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3661 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3721 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3781 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3841 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3901 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3961 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4021 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4081 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4141 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4201 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4261 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4321 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4381 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4441 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4501 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4561 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4621 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4681 gcgagtcagt gagcgaggaa gcggaaga
//
