LOCUS       Exported                4435 bp ds-DNA     circular SYN 25-APR-2019
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    pCfB8740 (pUC19-XdCrtI<-)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2019 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(271..2019)
                     /label=XdCrtI
                     /label=ORF1
                     /label=SsGGPS7
     primer_bind     complement(2039..2056)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2039..2055)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2048..2070)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2264..2283)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2383..2405
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2443..2461)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2529..2633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2634..3494
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     primer_bind     complement(2852..2871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    3665..4253
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4154..4173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4407..4424
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatcccc atgggaaaag aacaagatca ggataaaccc
      301 acagctatca tcgtgggatg tggtatcggt ggaatcgcca ctgccgctcg tcttgctaaa
      361 gaaggtttcc aggtcacggt gttcgagaag aacgactact ccggaggtcg atgctcttta
      421 atcgagcgag atggttatcg attcgatcag gggcccagtt tgctgctctt gccagatctc
      481 ttcaagcaga cattcgaaga tttgggggag aagatggaag attgggtcga tctcatcaag
      541 tgtgaaccca actatgtttg ccacttccac gatgaagaga ctttcacttt ttcaaccgac
      601 atggcgttgc tcaagcggga agtcgagcgt tttgaaggca aagatggatt tgatcggttc
      661 ttgtcgttta tccaagaagc ccacagacat tacgagcttg ctgtcgttca cgtcctgcag
      721 aagaacttcc ctggcttcgc agcattctta cggctacagt tcattggcca aatcctggct
      781 cttcacccct tcgagtctat ctggacaaga gtttgtcgat atttcaagac cgacagatta
      841 cgaagagtct tctcgtttgc agtgatgtac atgggtcaaa gcccatacag tgcgcccgga
      901 acatattcct tgctccaata caccgaattg accgagggca tctggtatcc gagaggaggc
      961 ttttggcagg ttcctaatac tcttcttcag atcgtcaagc gcaacaatcc ctcagccaag
     1021 ttcaatttca acgctccagt ttcccaggtt cttctctctc ctgccaagga ccgagcgact
     1081 ggtgttcgac ttgaatccgg cgaggaacat cacgccgatg ttgtgattgt caatgctgac
     1141 ctcgtttacg cctccgagca cttgattcct gacgatgcca gaaacaagat tggccaactg
     1201 ggtgaagtca agagaagttg gtgggctgac ttagttggtg gaaagaagct caagggaagt
     1261 tgcagtagtt tgagcttcta ctggagcatg gaccgaatcg tggacggtct gggcggacac
     1321 aatatcttct tggccgagga cttcaaggga tcattcgaca caatcttcga ggagttgggt
     1381 ctcccagccg atccttcctt ttacgtgaac gttccctcgc gaatcgatcc ttctgccgct
     1441 cccgaaggca aagatgctat cgtcattctt gtgccgtgtg gccatatcga cgcttcgaac
     1501 cctcaagatt acaacaagct tgttgctcgg gcaaggaagt ttgtgatcca aacgctttcc
     1561 gccaagcttg gacttcccga ctttgaaaaa atgattgtgg cagagaaggt tcacgatgct
     1621 ccctcttggg agaaagaatt taacctcaag gacggaagca tcttgggact ggctcacaac
     1681 tttatgcaag ttcttggttt caggccgagc accagacatc ccaagtatga caagttgttc
     1741 tttgtcgggg cttcgactca tcccggaact ggggttccca tcgtcttggc tggagccaag
     1801 ttaactgcca accaagttct cgaatccttt gaccgatccc cagctccaga tcccaatatg
     1861 tcactctccg taccatatgg aaaacctctc aaatcaaatg gaacgggtat cgattctcag
     1921 gtccagctga agttcatgga tttggagaga tgggtatacc ttttggtgtt gttgattggg
     1981 gccgtgatcg ctcgatccgt tggtgttctt gctttctgag ggtaccgagc tcgaattcac
     2041 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     2101 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2161 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     2221 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     2281 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     2341 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     2401 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     2461 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2521 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2581 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2641 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2701 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2761 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2821 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2881 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2941 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3001 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3061 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3121 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3181 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3241 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3301 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3361 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3421 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3481 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3541 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3601 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3781 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4321 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4381 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//
