LOCUS       pCfB8622      5248 bp ds-DNA     circular SYN 06-FEB-2018
DEFINITION  Cloning vector pESC-LEU, complete sequence.
ACCESSION   AF063849
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Lu Q.
  TITLE     pESC-LEU
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 4859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 6, 2018 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(1..20)
                     /label=TJOS-101R
     misc_feature    29..48
                     /label=TJOS-101F
     misc_feature    complement(99..118)
                     /label=TJOS-102R
     source          127..1336
                     /organism="unspecified"
                     /mol_type="genomic DNA"
                     /label=source:unspecified
     misc_signal     127..1336
                     /gene="(null)"
                     /label=(null)
     misc_feature    127..513
                     /gene="TEF1p"
                     /product="TEF1 promoter of A. gossypii"
                     /label="TEF1 promoter of A. gossypii"
     CDS             515..1087
                     /gene="nat"
                     /product="Nourseothricin acetyltransferase"
                     /label="Nourseothricin acetyltransferase"
                     /note="nat"
     misc_feature    1088..1336
                     /gene="TEF1t"
                     /product="TEF1 terminator of A. gossypii"
                     /label="TEF1 terminator of A. gossypii"
     misc_feature    1345..1364
                     /label=TJOS-102F
     misc_feature    complement(1415..1434)
                     /label=TJOS-97R
     misc_feature    1429..1434
                     /label=PvuII
     promoter        1448..1716
                     /label=pSNR52
     promoter        1717..1736
                     /label="ADE2 gRNA"
     promoter        1737..1815
                     /label="structural crRNA"
     promoter        1816..1835
                     /label=tSUP4
     misc_feature    1848..1867
                     /label=TJOS-97F
     misc_feature    1848..1853
                     /label=PvuII
                     /label=PvuII(1)
     rep_origin      2031..2698
                     /label="pUC ori"
                     /label="pUC ori"
                     /note="/vntifkey=33"
     CDS             complement(2849..3706)
                     /label=AmpR
                     /note="/vntifkey=4"
     rep_origin      3840..4995
                     /label="2 micron ori"
                     /label="2 micron ori"
                     /note="/vntifkey=33"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGAT
      121 TTAAATGATC TGTTTAGCTT GCCTCGTCCC CGCCGGGTCA CCCGGCCAGC GACATGGAGG
      181 CCCAGAATAC CCTCCTTGAC AGTCTTGACG TGCGCAGCTC AGGGGCATGA TGTGACTGTC
      241 GCCCGTACAT TTAGCCCATA CATCCCCATG TATAATCATT TGCATCCATA CATTTTGATG
      301 GCCGCACGGC GCGAAGCAAA AATTACGGCT CCTCGCTGCA GACCTGCGAG CAGGGAAACG
      361 CTCCCCTCAC AGACGCGTTG AATTGTCCCC ACGCCGCGCC CCTGTAGAGA AATATAAAAG
      421 GTTAGGATTT GCCACTGAGG TTCTTCTTTC ATATACTTCC TTTTAAAATC TTGCTAGGAT
      481 ACAGTTCTCA CATCACATCC GAACATAAAC AACCATGGGT ACCACTCTTG ACGACACGGC
      541 TTACCGGTAC CGCACCAGTG TCCCGGGGGA CGCCGAGGCC ATCGAGGCAC TGGATGGGTC
      601 CTTCACCACC GACACCGTTT TCCGCGTCAC CGCCACCGGG GACGGCTTCA CCCTGCGGGA
      661 GGTGCCGGTG GACCCGCCCC TGACCAAGGT GTTCCCCGAC GACGAATCGG ACGACGAATC
      721 GGACGACGGG GAGGACGGCG ACCCGGACTC CCGGACGTTC GTCGCGTACG GGGACGACGG
      781 CGACCTGGCG GGCTTCGTGG TCGTCTCGTA CTCCGGCTGG AACCGCCGGC TGACCGTCGA
      841 GGACATCGAG GTCGCCCCGG AGCACCGGGG GCACGGGGTC GGGCGCGCGT TGATGGGGCT
      901 CGCGACGGAG TTCGCCCGCG AGCGGGGCGC CGGGCACCTC TGGCTGGAGG TCACCAACGT
      961 CAACGCACCG GCGATCCACG CGTACCGGCG GATGGGGTTC ACCCTCTGCG GCCTGGACAC
     1021 CGCCCTGTAC GACGGCACCG CCTCGGACGG CGAGCAGGCG CTCTACATGA GCATGCCCTG
     1081 CCCCTAATCA GTACTGACAA TAAAAAGATT CTTGTTTTCA AGAACTTGTC ATTTGTATAG
     1141 TTTTTTTATA TTGTAGTTGT TCTATTTTAA TCAAATGTTA GCGTGATTTA TATTTTTTTT
     1201 CGCCTCGACA TCATCTGCCC AGATGCGAAG TTAAGTGCGC AGAAAGTAAT ATCATGCGTC
     1261 AATCGTATGT GAATGCTGGT CGCTATACTG CTGTCGATTC GATACTAACG CCGCCATCCA
     1321 GTGTCGAAAA CGAGCTGCGG CCGCCTACAG GGCGCGTCGC GCCATTCGCC ATTCAGGCTG
     1381 CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGAATGC
     1441 GTGCGATTCT TTGAAAAGAT AATGTATGAT TATGCTTTCA CTCATATTTA TACAGAAACT
     1501 TGATGTTTTC TTTCGAGTAT ATACAAGGTG ATTACATGTA CGTTTGAAGT ACAACTCTAG
     1561 ATTTTGTAGT GCCCTCTTGG GCTAGCGGTA AAGGTGCGCA TTTTTTCACA CCCTACAATG
     1621 TTCTGTTCAA AAGATTTTGG TCAAACGCTG TAGAAGTGAA AGTTGGTGCG CATGTTTCGG
     1681 CGTTCGAAAC TTCTCCGCAG TGAAAGATAA ATGATCAATT GTAGAGACTA TCCACAGTTT
     1741 TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA
     1801 CCGAGTCGGT GGTGCTTTTT TTGTTTTTTA TGTCTTCGCG TGCATTCCAG CTGCATTAAT
     1861 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC
     1921 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG
     1981 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG
     2041 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
     2101 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
     2161 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
     2221 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
     2281 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
     2341 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
     2401 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
     2461 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
     2521 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
     2581 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
     2641 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
     2701 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
     2761 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
     2821 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
     2881 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
     2941 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC
     3001 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC
     3061 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA
     3121 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC
     3181 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT
     3241 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA
     3301 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
     3361 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG
     3421 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC
     3481 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT
     3541 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT
     3601 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
     3661 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC
     3721 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA
     3781 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAAC
     3841 GAAGCATCTG TGCTTCATTT TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA
     3901 ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC
     3961 CAACGAAGAA TCTGTGCTTC ATTTTTGTAA AACAAAAATG CAACGCGAGA GCGCTAATTT
     4021 TTCAAACAAA GAATCTGAGC TGCATTTTTA CAGAACAGAA ATGCAACGCG AGAGCGCTAT
     4081 TTTACCAACA AAGAATCTAT ACTTCTTTTT TGTTCTACAA AAATGCATCC CGAGAGCGCT
     4141 ATTTTTCTAA CAAAGCATCT TAGATTACTT TTTTTCTCCT TTGTGCGCTC TATAATGCAG
     4201 TCTCTTGATA ACTTTTTGCA CTGTAGGTCC GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT
     4261 CTATTTTCTC TTCCATAAAA AAAGCCTGAC TCCACTTCCC GCGTTTACTG ATTACTAGCG
     4321 AAGCTGCGGG TGCATTTTTT CAAGATAAAG GCATCCCCGA TTATATTCTA TACCGATGTG
     4381 GATTGCGCAT ACTTTGTGAA CAGAAAGTGA TAGCGTTGAT GATTCTTCAT TGGTCAGAAA
     4441 ATTATGAACG GTTTCTTCTA TTTTGTCTCT ATATACTACG TATAGGAAAT GTTTACATTT
     4501 TCGTATTGTT TTCGATTCAC TCTATGAATA GTTCTTACTA CAATTTTTTT GTCTAAAGAG
     4561 TAATACTAGA GATAAACATA AAAAATGTAG AGGTCGAGTT TAGATGCAAG TTCAAGGAGC
     4621 GAAAGGTGGA TGGGTAGGTT ATATAGGGAT ATAGCACAGA GATATATAGC AAAGAGATAC
     4681 TTTTGAGCAA TGTTTGTGGA AGCGGTATTC GCAATATTTT AGTAGCTCGT TACAGTCCGG
     4741 TGCGTTTTTG GTTTTTTGAA AGTGCGTCTT CAGAGCGCTT TTGGTTTTCA AAAGCGCTCT
     4801 GAAGTTCCTA TACTTTCTAG AGAATAGGAA CTTCGGAATA GGAACTTCAA AGCGTTTCCG
     4861 AAAACGAGCG CTTCCGAAAA TGCAACGCGA GCTGCGCACA TACAGCTCAC TGTTCACGTC
     4921 GCACCTATAT CTGCGTGTTG CCTGTATATA TATATACATG AGAAGAACGG CATAGTGCGT
     4981 GTTTATGCTT AAATGCGTAC TTATATGCGT CTATTTATGT AGGATGAAAG GTAGTCTAGT
     5041 ACCTCCTGTG ATATTATCCC ATTCCATGCG GGGTATCGTA TGCTTCCTTC AGCACTACCC
     5101 TTTAGCTGTT CTATATGCTG CCACTCCTCA ATTGGATTAG TCTCATCCTT CAATGCTATC
     5161 ATTTCCTTTG ATATTGGATC ATACTAAGAA ACCATTATTA TCATGACATT AACCTATAAA
     5221 AATAGGCGTA TCACGAGGCC CTTTCGTC
//
