LOCUS       pCfB8380(XII-5MF_XdCrtE)_      6680 bp    DNA     circular UNA 
FEATURES             Location/Qualifiers
     misc_feature    join(5919..5942,5943..6680,1..3755,complement(3756..3786))
                     /feature_type="PCR fragment"
                     /note="Forward primer name: PR-22421(USER_backbone_2_Fwd)"
                     /note="Forward primer: atcGCgTguATCCGCTCTAACCGAAAAGGAAG"
                     /note="Forward primer misprimes: 6"
                     /note="Reverse primer name: PR-22420(USER_backbone_1_Rev)"
                     /note="Reverse primer: ATcgCacguGTAGATACGTTGTTGACACTTC"
                     /note="Reverse primer misprimes: 6"
                     /note="Fragment length: 4556"
                     /label="Fragment (pCfB2909\(XII-5-MarkerFree) (PR-22421(USER_backbone_2_Fwd)  - PR-22420(USER_backbone_1_Rev) ))"
     misc_feature    join(6233..6680,1..228)
                     /vntifkey="21"
                     /label="XII-5 Down"
     rep_origin      1005..1670
                     /vntifkey="33"
                     /label="pUC ori"
     CDS             complement(1772..2632)
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    3091..3544
                     /vntifkey="21"
                     /label="XII-5 UP"
     terminator      3585..3746
                     /vntifkey="43"
                     /label="T ADH1"
     promoter        3787..4770
                     /label=pPGK1->
     misc_feature    join(4771..4800,4801..5889,complement(5890..5918))
                     /feature_type="PCR fragment"
                     /note="Forward primer name: TJOS-25F"
                     /note="Forward primer: AtctGtcauATGGATTACGCGAACATCCTC"
                     /note="Forward primer misprimes: 7"
                     /note="Reverse primer name: TJOS-25R"
                     /note="Reverse primer: cacgCGAuTCACAGAGGGATATCGGCTAG"
                     /note="Reverse primer misprimes: 5"
                     /note="Fragment length: 1148"
                     /label="Fragment (p1763 (TJOS-25F - TJOS-25R))"
     CDS             4780..5910
                     /cds_type=ORF
                     /label=CrtE
     terminator      5920..6108
                     /vntifkey="43"
                     /label="T CYC1"
     primer          complement(6091..6116)
                     /vntifkey="27"
                     /label=ANTISENSE_PRM
                     /note="Antisense primer used for creating BB0612
                     (XII-5_w/o_marker)"
     primer          6117..6141
                     /vntifkey="27"
                     /label=SENSE_PRM
                     /note="Sense primer used for creating BB0612
                     (XII-5_w/o_marker)"
ORIGIN
        1 TGTGCCTTCC CGTCGGGTAA GGGAGTTATT TAGGGATACA GAATCTAACG AAAACTAAAT
       61 CTCAATGATT AACTCTATTT AATCCTTTTT TGAAAGGCAA AAGAGGTCCC TTGTTCACTT
      121 ACAACGTTCT TAGCCAAATT CGCTTATCAC TTACTACTTC ACGATATACA GAAGTAAAAA
      181 CATATAAAAA GATGTCTGTT TGTTTAGCCA TCACAAAAGG TATCGCAGAT TAAGACCTCA
      241 GCGCGGCCGC AAATTTAAAT AAAATGAAGT GAAGTTCCTA TACTTTCTAG AGAATAGGAA
      301 CTTCTATAGT GAGTCGAATA AGGGCGACAC AAAATTTATT CTAAATGCAT AATAAATACT
      361 GATAACATCT TATAGTTTGT ATTATATTTT GTATTATCGT TGACATGTAT AATTTTGATA
      421 TCAAAAACTG ATTTTCCCTT TATTATTTTC GAGATTTATT TTCTTAATTC TCTTTAACAA
      481 ACTAGAAATA TTGTATATAC AAAAAATCAT AAATAATAGA TGAATAGTTT AATTATAGGT
      541 GTTCATCAAT CGAAAAAGCA ACGTATCTTA TTTAAAGTGC GTTGCTTTTT TCTCATTTAT
      601 AAGGTTAAAT AATTCTCATA TATCAAGCAA AGTGACAGGC GCCCTTAAAT ATTCTGACAA
      661 ATGCTCTTTC CCTAAACTCC CCCCATAAAA AAACCCGCCG AAGCGGGTTT TTACGTTATT
      721 TGCGGATTAA CGATTACTCG TTATCAGAAC CGCCCAGGGG GCCCGAGCTT AAGACTGGCC
      781 GTCGTTTTAC AACACAGAAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGGGCCT
      841 TCTGCTTAGT TTGATGCCTG GCAGTTCCCT ACTCTCGCCT TCCGCTTCCT CGCTCACTGA
      901 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT
      961 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
     1021 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
     1081 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1141 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1201 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1261 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1321 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1381 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1441 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGGCT AACTACGGCT ACACTAGAAG
     1501 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1561 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1621 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     1681 CGCTCAGTGG AACGACGCGC GCGTAACTCA CGTTAAGGGA TTTTGGTCAT GAGCTTGCGC
     1741 CGTCCCGTCA AGTCAGCGTA ATGCTCTGCT TTTACCAATG CTTAATCAGT GAGGCACCTA
     1801 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
     1861 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGCGCTGC GATGATACCG CGAGAACCAC
     1921 GCTCACCGGC TCCGGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
     1981 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
     2041 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATCGCTACA GGCATCGTGG
     2101 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
     2161 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
     2221 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
     2281 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
     2341 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
     2401 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
     2461 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
     2521 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
     2581 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATATTCTTCC
     2641 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
     2701 AATGTATTTA GAAAAATAAA CAAATAGGGG TCAGTGTTAC AACCAATTAA CCAATTCTGA
     2761 ACATTATCGC GAGCCCATTT ATACCTGAAT ATGGCTCATA ACACCCCTTG TTTGCCTGGC
     2821 GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT CAGAAGTGAA ACGCCGTAGC
     2881 GCCGATGGTA GTGTGGGGAC TCCCCATGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA
     2941 ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGCCCGGGC TAATTATGGG GTGTCGCCCT
     3001 TATTCGACTC TATAGTGAAG TTCCTATTCT CTAGAAAGTA TAGGAACTTC TGAAGTGGGG
     3061 ATTTAAATGC GGCCGCGCTG AGGGTTTAAT CAATCTGGCG GCTTGAGTTC TCAACATGTT
     3121 TTATTTTTTA CTTATATTGC TGGTAGGGTA AAAAAATATA ACTCCTAGGA ATAGGTTGTC
     3181 TATATGTTTT TGTCTTGCTT CTATAATTGT AACAAACAAG GAAAGGGAAA ATACTGGGTG
     3241 TAAAAGCCAT TGAGTCAAGT TAGGTCATCC CTTTTATACA AAATTTTTCA ATTTTTTTTC
     3301 CAAGATTCTT GTACGATTAA TTATTTTTTT TTTGCGTCCT ACAGCGTGAT GAAAATTTCG
     3361 CCTGCTGCAA GATGAGCGGG AACGGGCGAA ATGTGCACGC GCACAACTTA CGAAACGCGG
     3421 ATGAGTCACT GACAGCCACC GCAGAGGTTC TGACTCCTAC TGAGCTCTAT TGGAGGTGGC
     3481 AGAACCGGTA CCGGAGGAGG CCGCTATAAC CGGTTTGAAT TTATTGTCAC AGTGTCACAT
     3541 CAGCATTAAG TCCTCAGCGA GCTCGCATGG AATGCGTGCG ATGAGCGACC TCATGCTATA
     3601 CCTGAGAAAG CAACCTGACC TACAGGAAAG AGTTACTCAA GAATAAGAAT TTTCGTTTTA
     3661 AAACCTAAGA GTCACTTTAA AATTTGTATA CACTTATTTT TTTTATAACT TATTTAATAA
     3721 TAAAAATCAT AAATCATAAG AAATTCGCTT ATTTAGAAGT GTCAACAACG TATCTACACG
     3781 TGCGATGGAA GTACCTTCAA AGAATGGGGT CTTATCTTGT TTTGCAAGTA CCACTGAGCA
     3841 GGATAATAAT AGAAATGATA ATATACTATA GTAGAGATAA CGTCGATGAC TTCCCATACT
     3901 GTAATTGCTT TTAGTTGTGT ATTTTTAGTG TGCAAGTTTC TGTAAATCGA TTAATTTTTT
     3961 TTTCTTTCCT CTTTTTATTA ACCTTAATTT TTATTTTAGA TTCCTGACTT CAACTCAAGA
     4021 CGCACAGATA TTATAACATC TGCATAATAG GCATTTGCAA GAATTACTCG TGAGTAAGGA
     4081 AAGAGTGAGG AACTATCGCA TACCTGCATT TAAAGATGCC GATTTGGGCG CGAATCCTTT
     4141 ATTTTGGCTT CACCCTCATA CTATTATCAG GGCCAGAAAA AGGAAGTGTT TCCCTCCTTC
     4201 TTGAATTGAT GTTACCCTCA TAAAGCACGT GGCCTCTTAT CGAGAAAGAA ATTACCGTCG
     4261 CTCGTGATTT GTTTGCAAAA AGAACAAAAC TGAAAAAACC CAGACACGCT CGACTTCCTG
     4321 TCTTCCTATT GATTGCAGCT TCCAATTTCG TCACACAACA AGGTCCTAGC GACGGCTCAC
     4381 AGGTTTTGTA ACAAGCAATC GAAGGTTCTG GAATGGCGGG AAAGGGTTTA GTACCACATG
     4441 CTATGATGCC CACTGTGATC TCCAGAGCAA AGTTCGTTCG ATCGTACTGT TACTCTCTCT
     4501 CTTTCAAACA GAATTGTCCG AATCGTGTGA CAACAACAGC CTGTTCTCAC ACACTCTTTT
     4561 CTTCTAACCA AGGGGGTGGT TTAGTTTAGT AGAACCTCGT GAAACTTACA TTTACATATA
     4621 TATAAACTTG CATAAATTGG TCAATGCAAG AAATACATAT TTGGTCTTTT CTAATTCGTA
     4681 GTTTTTCAAG TTCTTAGATG CTTTCTTTTT CTCTTTTTTA CAGATCATCA AGGAAGTAAT
     4741 TATCTACTTT TTACAACAAA TATAAAACAA ATCTGTCATA TGGATTACGC GAACATCCTC
     4801 acagcaattc cactcgagtt tactcctcag gatgatatcg tgctccttga accgtatcac
     4861 tacctaggaa agaaccctgg aaaagaaatt cgatcacaac tcatcgaggc tttcaactat
     4921 tggttggatg tcaagaagga ggatctcgag gtcatccaga acgttgttgg catgctacat
     4981 accgctagct tattaatgga cgatgtggag gattcatcgg tcctcaggcg tgggtcgcct
     5041 gtggcccatc taatttacgg gattccgcag acaataaaca ctgcaaacta cgtctacttt
     5101 ctggcttatc aagagatctt caagcttcgc ccaacaccga tacccatgcc tgtaattcct
     5161 ccttcatctg cttcgcttca atcatccgtc tcctctgcat cctcctcctc ctcggcctcg
     5221 tctgaaaacg ggggcacgtc aactcctaat tcgcagattc cgttctcgaa agatacgtat
     5281 cttgataaag tgatcacaga cgagatgctt tccctccata gagggcaagg cctggagcta
     5341 ttctggagag atagtctgac gtgtcctagc gaagaggaat atgtgaaaat ggttcttgga
     5401 aagacgggag gtttgttccg tatagcggtc agattgatga tggcaaagtc agaatgtgac
     5461 atagactttg tccagcttgt caacttgatc tcaatatact tccagatcag ggatgactat
     5521 atgaaccttc agtcttctga gtatgcccat aataagaatt ttgcagagga cctcacagaa
     5581 gggaaattca gttttcccac tatccactcg attcatgcca acccctcatc gagactcgtc
     5641 atcaatacgt tgcagaagaa atcgacctct cctgagatcc ttcaccactg tgtaaactac
     5701 atgcgcacag aaacccactc attcgaatat actcaggaag tcctcaacac cttgtcaggt
     5761 gcactcgaga gagaactagg aaggcttcaa ggagagttcg cagaagctaa ctcaaggatg
     5821 gatcttggag acgtagattc ggaaggaaga acggggaaga acgtcaaatt ggaagcgatc
     5881 ctgaaaaagC TAGCCGATAT CCCTCTGTGA ATCGCGTGTA TCCGCTCTAA CCGAAAAGGA
     5941 AGGAGTTAGA CAACCTGAAG TCTAGGTCCC TATTTATTTT TTTATAGTTA TGTTAGTATT
     6001 AAGAACGTTA TTTATATTTC AAATTTTTCT TTTTTTTCTG TACAGACGCG TGTACGCATG
     6061 TAACATTATA CTGAAAACCT TGCTTGAGAA GGTTTTGGGA CGCTCGAAGA TCGCGTACCC
     6121 AATTCGCCCT ATAGTGAGTC GTATTACGCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT
     6181 GACTGGGAAA ACCCTGGCGT TACCCCTGCA GGACTAGTGC TGAGGCATTA ATGCAACTCA
     6241 GAAGTTTGAC AGCAAGCAAG TTCATCATTC GAACTAGCCT TATTGTTTTA GTTCAGTGAC
     6301 AGCGAACTGC CGTACTCGAT GCTTTATTTC TCACGGTAGA GCGGAAGAAC AGATAGGGGC
     6361 AGCGTGAGAA GAGTTAGAAA GTAAATTTTT ATCACGTCTG AAGTATTCTT ATTCATAGGA
     6421 AATTTTGCAA GGTTTTTTAG CTCAATAACG GGCTAAGTTA TATAAGGTGT TCACGCGATT
     6481 TTCTTGTTAT GTATACCTCT TCTCTGAGGA ATGGTACTAC TGTCCTGATG TAGGCTCCTT
     6541 AAATTGGTGG GCAAGAATAA CTTATCGATA TTTTGTATAT TGGTCTTGGA GTTCACCACG
     6601 TAATGCCTGT TTAAGACCAT CAGTTAACTC TAGTATTATT TGGTCTTGGC TACTGGCCGT
     6661 TTGCTATTAT TCAAGTCTTT
//
