LOCUS       Exported                7098 bp ds-DNA     circular SYN 25-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB8748_(pIntE_1-TPex20-PrFBA1-XdCrtE-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2019 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    6..13
                     /label=USER Linker yellow
     misc_feature    14..513
                     /label=IntE-1_up
     terminator      complement(527..839)
                     /label=TPex20
     primer_bind     850..882
                     /label=PrYlFBA1 _fw (ID15523)
     promoter        858..1686
                     /label=PrFBA1
     primer_bind     complement(1662..1695)
                     /label=PrYlFBA1 _rev (ID15524)
     primer_bind     1687..1716
                     /label=Pr-7043 (TJOS-25F )
     gene            1696..2826
                     /label=XdCrtE
     primer_bind     complement(2806..2834)
                     /label=TJOS-25R (PR-7044)
     terminator      2840..3776
                     /label=T lip2
     primer_bind     complement(3758..3776)
                     /label=PR-15781
     misc_feature    3785..4251
                     /label=IntE-1_down
     misc_feature    4252..4259
                     /label=USER Linker red
     misc_feature    4266..7098
                     /label=vector backbone
ORIGIN
        1 gaatgcgtgc gatttgatat ggtgtaacaa tgataaacca aggcccattg attgagacgg
       61 agatattttc ggcgtgtcta gggtgtctac ttgtgcaccg gaacatggaa gatcgtgcgc
      121 tgttcacttg caacagaagg ctaacgagag aaactgggcc aacccgtcta tgctcttcat
      181 gctactcaat ctattcaaaa tacgccagtg ctccccttgc cttatacaca ggctcttcac
      241 tcacatcttg accatcttgg atgtgggtac tgtagctcct gggaaccagt cgtgcgggag
      301 catgagaacc acgtcgaaga agatggtgac ccaggaaagc aaaaacttgg gcacattctt
      361 gtagataccc tgacctgtcg tggtcgaaga taccgtcgaa aaccagctgt cccattgaca
      421 gaaaccctcc tcctgtatct agcagaacgc tggcaataga aaagccatta gtggactttc
      481 gcgtgtagtt cgacgcagca gaggatagtg cttacgtgca acgcttacgc aactaacatg
      541 aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaaga
      601 gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt
      661 atgtttagta ttgtacttgg acagtgcttg tatcgtacat tctcaagtgt caaacataaa
      721 tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat
      781 ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttc
      841 aacggaatgc gtgcgataac agtgtacgca gtactataga ggaacaattg ccccggagaa
      901 gacggccagg ccgcctagat gacaaattca acaactcaca gctgactttc tgccattgcc
      961 actagggggg ggccttttta tatggccaag ccaagctctc cacgtcggtt gggctgcacc
     1021 caacaataaa tgggtagggt tgcaccaaca aagggatggg atggggggta gaagatacga
     1081 ggataacggg gctcaatggc acaaataaga acgaatactg ccattaagac tcgtgatcca
     1141 gcgactgaca ccattgcatc atctaagggc ctcaaaacta cctcggaact gctgcgctga
     1201 tctggacacc acagaggttc cgagcacttt aggttgcacc aaatgtccca ccaggtgcag
     1261 gcagaaaacg ctggaacagc gtgtacagtt tgtcttagca aaaagtgaag gcgctgaggt
     1321 cgagcagggt ggtgtgactt gttatagcct ttagagctgc gaaagcgcgt atggatttgg
     1381 ctcatcaggc cagattgagg gtctgtggac acatgtcatg ttagtgtact tcaatcgccc
     1441 cctggatata gccccgacaa taggccgtgg cctcattttt ttgccttccg cacatttcca
     1501 ttgctcggta cccacacctt gcttctcctg cacttgccaa ccttaatact ggtttacatt
     1561 gaccaacatc ttacaagcgg ggggcttgtc tagggtatat ataaacagtg gctctcccaa
     1621 tcggttgcca gtctcttttt tcctttcttt ccccacagat tcgaaatcta aactacacat
     1681 cacacaatct gtcatatgga ttacgcgaac atcctcacag caattccact cgagtttact
     1741 cctcaggatg atatcgtgct ccttgaaccg tatcactacc taggaaagaa ccctggaaaa
     1801 gaaattcgat cacaactcat cgaggctttc aactattggt tggatgtcaa gaaggaggat
     1861 ctcgaggtca tccagaacgt tgttggcatg ctacataccg ctagcttatt aatggacgat
     1921 gtggaggatt catcggtcct caggcgtggg tcgcctgtgg cccatctaat ttacgggatt
     1981 ccgcagacaa taaacactgc aaactacgtc tactttctgg cttatcaaga gatcttcaag
     2041 cttcgcccaa caccgatacc catgcctgta attcctcctt catctgcttc gcttcaatca
     2101 tccgtctcct ctgcatcctc ctcctcctcg gcctcgtctg aaaacggggg cacgtcaact
     2161 cctaattcgc agattccgtt ctcgaaagat acgtatcttg ataaagtgat cacagacgag
     2221 atactttccc tccatagagg gcaaggcctg gagctattct ggagagatag tctgacgtgt
     2281 cctagcgaag aggaatatgt gaaaatggtt cttggaaaga cgggaggttt gttccgtata
     2341 gcggtcagat tgatgatggc aaagtcagaa tgtgacatag actttgtcca gcttgtcaac
     2401 ttgatctcaa tatacttcca gatcagggat gactatatga accttcagtc ttctgagtat
     2461 gcccataata agaattttgc agaggacctc acagaaggga aattcagttt tcccactatc
     2521 cactcgattc atgccaaccc ctcatcgaga ctcgtcatca atacgttgca gaagaaatcg
     2581 acctctcctg agatccttca ccactgtgta aactacatgc gcacagaaac ccactcattc
     2641 gaatatactc aggaagtcct caacaccttg tcaggtgcac tcgagagaga actaggaagg
     2701 cttcaaggag agttcgcaga agctaactca aggatggatc ttggagacgt agattcggaa
     2761 ggaagaacgg ggaagaacgt caaattggaa gcgatcctga aaaagctagc cgatatccct
     2821 ctgtgaatcg cgtgcattcc ttctgttcgg aatcaacctc aaggttaacg gccacgatcc
     2881 cctcgttgtt actcttggtc agcccattgt cggtaacgct ggctttgcta actgggtcga
     2941 taaactcttc tttggccagg agaaccccga tgtctccaag gtgtccaaag accgaaagct
     3001 ctaccgaatc acccaccgag gagatatcgt ccctcaagtg cccttctggg acggttacca
     3061 gcactgctct ggtgaggtct ttattgactg gcccctgatc caccctcctc tctccaacgt
     3121 tgtcatgtgc cagggccaga gcaataaaca gtgctctgcc ggtaacactc tgctccagca
     3181 ggtcaatgtg attggaaacc atctgcagta cttcgtcacc gagggtgtct gtggtatcta
     3241 agctatttat cactctttac aacttctacc tcaactatct actttaataa atgaatatcg
     3301 tttattctct atgattactg tatatgcgtt cctctaagac aaatcgaaac cagcatgcga
     3361 tcgaatggca tacaaaagtt tcttccgaag ttgatcaatg tcctgatagt caggcagctt
     3421 gagaagattg acacaggtgg aggccgtagg gaaccgatca acctgtctac cagcgttacg
     3481 aatggcaaat gacgggttca aagccttgaa tccttgcaat ggtgccttgg atactgatgt
     3541 cacaaactta agaagcagcc gcttgtcctc ttcctcgaaa ctctcaaaca cagtccagaa
     3601 gtcctttata gtttgatctg tatccagata gcctccgtaa ttggtgtgtg tcttcaaatc
     3661 ccagacgtcc acattggcat gtcctccact gataagcatt tgaagttcat ctgcgttgaa
     3721 cattgagacc cacgaagggt caatgagctg gtatagaccg cccaagaatg catctgagtg
     3781 gcctttgtgt cgaaatacaa cagccagtcc tccacgatcc agctcagata gccctgggtg
     3841 ttgagataga gacaagtcga gcgagatgtt ctcagtggac ttgagtttga tgttgtgaaa
     3901 gatggttgga ttgagagcac aactccatag aacatagtag tctcagtcca tgagctagga
     3961 gagaatctcc agtgctgatg tcatttgaag aagaagagga agaagaagac agatagatca
     4021 actcagcccc ttccagttgc cgtacaatga ctcaaccaaa tacgttcaag cctgctttca
     4081 agcctgcgtg ctctttctgt ctcattagtt gaacgtagat catctgagag aacttggcag
     4141 accgtgcaaa cacaagggcg agactgatag gacacttgat ctgatccaaa atttgacaga
     4201 ggaaaactag ttttgaagtt actgaacgtt tcttctccaa ttgcttctaa catcgcgtgc
     4261 attcgcggcc gcatttaaat ccccacttca gaagttccta tactttctag agaataggaa
     4321 cttcactata gagtcgaata agggcgacac cccataatta gcccgggcga aaggcccagt
     4381 ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga
     4441 gtccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt
     4501 gggaccaccg cgctactgcc gccaggcaaa caaggggtgt tatgagccat attcaggtat
     4561 aaatgggctc gcgataatgt tcagaattgg ttaattggtt gtaacactga cccctatttg
     4621 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4681 gcttcaataa tattgaaaaa ggaagaatat gagtattcaa catttccgtg tcgcccttat
     4741 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4801 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4861 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4921 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4981 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5041 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5101 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5161 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5221 accaaacgac gagcgtgaca ccacgatgcc tgtagcgatg gcaacaacgt tgcgcaaact
     5281 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5341 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5401 taaatccgga gccggtgagc gtggttctcg cggtatcatc gcagcgctgg ggccagatgg
     5461 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5521 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaaa agcagagcat
     5581 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
     5641 gcgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5701 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5761 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5821 cgcagatacc aaatactgtt cttctagtgt agccgtagtt agcccaccac ttcaagaact
     5881 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5941 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6001 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6061 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6301 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6361 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6421 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aggcgagagt agggaactgc
     6481 caggcatcaa actaagcaga aggcccctga cggatggcct ttttgcgttt ctacaaactc
     6541 tttctgtgtt gtaaaacgac ggccagtctt aagctcgggc cccctgggcg gttctgataa
     6601 cgagtaatcg ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggg
     6661 ggagtttagg gaaagagcat ttgtcagaat atttaagggc gcctgtcact ttgcttgata
     6721 tatgagaatt atttaacctt ataaatgaga aaaaagcaac gcactttaaa taagatacgt
     6781 tgctttttcg attgatgaac acctataatt aaactattca tctattattt atgatttttt
     6841 gtatatacaa tatttctagt ttgttaaaga gaattaagaa aataaatctc gaaaataata
     6901 aagggaaaat cagtttttga tatcaaaatt atacatgtca acgataatac aaaatataat
     6961 acaaactata agatgttatc agtatttatt atgcatttag aataaatttt gtgtcgccct
     7021 tattcgactc actatagaag ttcctattct ctagaaagta taggaacttc acttcatttt
     7081 atttaaattt gcggccgc
//
