LOCUS       Exported                7841 bp ds-DNA     circular SYN 25-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCfB8786_(pIntD_1-TPex20-XdCrtI-PrGPD-TLip2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 25, 2019 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    6..13
                     /label=USER Linker yellow
                     /note="Oriinal location: 6..13"
     misc_feature    14..513
                     /label=Int_D1 up
                     /note="Oriinal location: 14..533"
     primer_bind     514..526
                     /label=PR-10718 (TPex20USER _fw)
                     /note="Oriinal location: 534..546"
     terminator      complement(527..839)
                     /label=TPex20
                     /note="Oriinal location: complement(547..859)"
     primer_bind     761..777
                     /label=PR-14617
     gene            complement(858..2606)
                     /label=XdCrtI
     primer_bind     858..879
                     /label=PR-7042 (TJOS-24R )
     primer_bind     complement(2585..2606)
                     /label=PR-7041(TJOS 24F)
     primer_bind     2607..2634
                     /label=PR-13338(PrGPD_rv)
     promoter        complement(2616..3547)
                     /label=PrGDP
     primer_bind     complement(2677..2693)
                     /label=PR-14441 (PrGPD_seqcheck_fw)
     primer_bind     complement(3529..3555)
                     /label=PR-13337 (PrGPD _fw)
     terminator      3561..4497
                     /label=T lip2
                     /note="Oriinal location: 889..1825"
     misc_feature    4506..4994
                     /label=IntD_1 down
                     /note="Oriinal location: 3275..3774"
     misc_feature    4995..5002
                     /label=USER Linker red
                     /note="Oriinal location: 3775..3782"
     misc_feature    5009..7841
                     /label=vector backbone
                     /note="Oriinal location: 3789..6621"
ORIGIN
        1 gaatgcgtgc gatcattttc ccgtcatatc aaacacatca ccaacgctcg taattagcat
       61 cggttcgcta acgtattcca aacacggatt cgagcacctc aacaacatgt ctcactagcg
      121 cgcatgacgc aaaacttctc caaagcacgg gtacataggg catgtgtctg ttagcgtctc
      181 gacaaggccg caattactta tgaaacctca tcggctgttt cagggggcga gtggcttgca
      241 catttgctgt taataccgag ataacggcgt gtgaggtata gtacaatgat tacatcgagg
      301 agcgatcaga gagaacagaa caagtcggaa gatgaacgca atcattttga cataaaatat
      361 cagagcggaa aaatcagttg tttttattta tttatttttt cagaatggca ccatggatct
      421 acctcgagtc taactacacc aacataatta gtgggtatgg tcgttgagtg ttgacaaatt
      481 agtgggaaca atgcagagtg ttagagcaat gtcacgtgca acgcttacgc aactaacatg
      541 aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaaga
      601 gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt
      661 atgtttagta ttgtacttgg acagtgcttg tatcgtacat tctcaagtgt caaacataaa
      721 tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat
      781 ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttc
      841 aacggaatgc gtgcgattca gaaagcaaga acaccaacgg atcgagcgat cacggcccca
      901 atcaacaaca ccaaaaggta tacccatctc tccaaatcca tgaacttcag ctggacctga
      961 gaatcgatac ccgttccatt tgatttgaga ggttttccat atggtacgga gagtgacata
     1021 ttgggatctg gagctgggga tcggtcaaag gattcgagaa cttggttggc agttaacttg
     1081 gctccagcca agacgatggg aaccccagtt ccgggatgag tcgaagcccc gacaaagaac
     1141 aacttgtcat acttgggatg tctggtgctc ggcctgaaac caagaacttg cataaagttg
     1201 tgagccagtc ccaagatgct tccgtccttg aggttaaatt ctttctccca agagggagca
     1261 tcgtgaacct tctctgccac aatcattttt tcaaagtcgg gaagtccaag cttggcggaa
     1321 agcgtttgga tcacaaactt ccttgcccga gcaacaagct tgttgtaatc ttgagggttc
     1381 gaagcgtcga tatggccaca cggcacaaga atgacgatag catctttgcc ttcgggagcg
     1441 gcagaaggat cgattcgcga gggaacgttc acgtaaaagg aaggatcggc tgggagaccc
     1501 aactcctcga agattgtgtc gaatgatccc ttgaagtcct cggccaagaa gatattgtgt
     1561 ccgcccagac cgtccacgat tcggtccatg ctccagtaga agctcaaact actgcaactt
     1621 cccttgagct tctttccacc aactaagtca gcccaccaac ttctcttgac ttcacccagt
     1681 tggccaatct tgtttctggc atcgtcagga atcaagtgct cggaggcgta aacgaggtca
     1741 gcattgacaa tcacaacatc ggcgtgatgt tcctcgccgg attcaagtcg aacaccagtc
     1801 gctcggtcct tggcaggaga gagaagaacc tgggaaactg gagcgttgaa attgaacttg
     1861 gctgagggat tgttgcgctt gacgatctga agaagagtat taggaacctg ccaaaagcct
     1921 cctctcggat accagatgcc ctcggtcaat tcggtgtatt ggagcaagga atatgttccg
     1981 ggcgcactgt atgggctttg acccatgtac atcactgcaa acgagaagac tcttcgtaat
     2041 ctgtcggtct tgaaatatcg acaaactctt gtccagatag actcgaaggg gtgaagagcc
     2101 aggatttggc caatgaactg tagccgtaag aatgctgcga agccagggaa gttcttctgc
     2161 aggacgtgaa cgacagcaag ctcgtaatgt ctgtgggctt cttggataaa cgacaagaac
     2221 cgatcaaatc catctttgcc ttcaaaacgc tcgacttccc gcttgagcaa cgccatgtcg
     2281 gttgaaaaag tgaaagtctc ttcatcgtgg aagtggcaaa catagttggg ttcacacttg
     2341 atgagatcga cccaatcttc catcttctcc cccaaatctt cgaatgtctg cttgaagaga
     2401 tctggcaaga gcagcaaact gggcccctga tcgaatcgat aaccatctcg ctcgattaaa
     2461 gagcatcgac ctccggagta gtcgttcttc tcgaacaccg tgacctggaa accttcttta
     2521 gcaagacgag cggcagtggc gattccaccg ataccacatc ccacgatgat agctgtgggt
     2581 ttatcctgat cttgttcttt tcccatacct gcactgttga tgtgtgttta attcaagaat
     2641 gaatatagag aagagaagaa gaaaaaagat tcaattgagc cggcgatgca gacccttata
     2701 taaatgttgc cttggacaga cggagcaagc ccgcccaaac ctacgttcgg tataatatgt
     2761 taagcttttt aacacaaagg tttggcttgg ggtaacctga tgtggtgcaa aagaccgggc
     2821 gttggcgagc cattgcgcgg gcgaatgggg ccgtgactcg tctcaaattc gagggcgtgc
     2881 ctcaattcgt gcccccgtgg ctttttcccg ccgtttccgc cccgtttgca ccactgcagc
     2941 cgcttctttg gttcggacac cttgctgcga gctaggtgcc ttgtgctact taaaaagtgg
     3001 cctcccaaca ccaacatgac atgagtgcgt gggccaagac acgttggcgg ggtcgcagtc
     3061 ggctcaatgg cccggaaaaa acgctgctgg agctggttcg gacgcagtcc gccgcggcgt
     3121 atcgatatcc gcaaggttcc atggcgccat tgccctccgt cggcgtctat cccgcaacct
     3181 ctaaatagag cgggaatata acccaagctt cttttttttt cctttaacac gcacaccccc
     3241 aactatcatg ttgctgctgc tgtttgactc tactctgtgg aggggtgctc ccacccaacc
     3301 caacctacag gtggatccgg cgctgtgatt ggctgataag tctcctatcc ggactaattc
     3361 tgaccaatgg gacatgcgcg caggacccaa atgccgcaat tacgtaaccc caacgaaatg
     3421 cctacccctc tttggagccc agcggcccca aatcccccca agcagcccgg ttctaccggc
     3481 ttccatctcc aagcacccct ttctccacac cccacaaaaa gacccgtgca ggacatccta
     3541 ctgcgtcatc gcgtgcattc cttctgttcg gaatcaacct caaggttaac ggccacgatc
     3601 ccctcgttgt tactcttggt cagcccattg tcggtaacgc tggctttgct aactgggtcg
     3661 ataaactctt ctttggccag gagaaccccg atgtctccaa ggtgtccaaa gaccgaaagc
     3721 tctaccgaat cacccaccga ggagatatcg tccctcaagt gcccttctgg gacggttacc
     3781 agcactgctc tggtgaggtc tttattgact ggcccctgat ccaccctcct ctctccaacg
     3841 ttgtcatgtg ccagggccag agcaataaac agtgctctgc cggtaacact ctgctccagc
     3901 aggtcaatgt gattggaaac catctgcagt acttcgtcac cgagggtgtc tgtggtatct
     3961 aagctattta tcactcttta caacttctac ctcaactatc tactttaata aatgaatatc
     4021 gtttattctc tatgattact gtatatgcgt tcctctaaga caaatcgaaa ccagcatgcg
     4081 atcgaatggc atacaaaagt ttcttccgaa gttgatcaat gtcctgatag tcaggcagct
     4141 tgagaagatt gacacaggtg gaggccgtag ggaaccgatc aacctgtcta ccagcgttac
     4201 gaatggcaaa tgacgggttc aaagccttga atccttgcaa tggtgccttg gatactgatg
     4261 tcacaaactt aagaagcagc cgcttgtcct cttcctcgaa actctcaaac acagtccaga
     4321 agtcctttat agtttgatct gtatccagat agcctccgta attggtgtgt gtcttcaaat
     4381 cccagacgtc cacattggca tgtcctccac tgataagcat ttgaagttca tctgcgttga
     4441 acattgagac ccacgaaggg tcaatgagct ggtatagacc gcccaagaat gcatctgagt
     4501 ggccttctgc gtctgctgtt tgtgtctttc tcgttcgcca cggtccgcct gagtcaccat
     4561 tgcccatcta ctccccggcc cagtcatcga ctttgagcga tgacgccggt taccggaacc
     4621 tttgcagggg caacgccgac accccggcgc gtgtggtcgc cattgcaacg gttcattcta
     4681 gcacatgtgc catgtggccg tgtcgcacct tgtatccacg cagctgctct ccgacatgcc
     4741 caaaccctcg cgcaatagtg tcaggtttac gagtgtcaat gcgatggcat aattagtgtt
     4801 tccaaccaag cgatattata gccaacatga aagcgaggtt gacgggcgac gacgcgacgg
     4861 ctgagttgct gagccttgtg gaaacggcgg ccattatata acgctcaaga agctcgaggc
     4921 gtgttttctg gcaaccacac cgactcggtg gcccattgca ccctccacac ctctccactc
     4981 ttacccctgg ccggatcgcg tgcattcgcg gccgcattta aatccccact tcagaagttc
     5041 ctatactttc tagagaatag gaacttcact atagagtcga ataagggcga caccccataa
     5101 ttagcccggg cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc
     5161 agttccctac tctcgcatgg ggagtcccca cactaccatc ggcgctacgg cgtttcactt
     5221 ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc aaacaagggg
     5281 tgttatgagc catattcagg tataaatggg ctcgcgataa tgttcagaat tggttaattg
     5341 gttgtaacac tgacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5401 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa tatgagtatt
     5461 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5521 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5581 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5641 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5701 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5761 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5821 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5881 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5941 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagcg
     6001 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6061 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6121 ccggctggct ggtttattgc tgataaatcc ggagccggtg agcgtggttc tcgcggtatc
     6181 atcgcagcgc tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6241 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6301 aagcattggt aaaagcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     6361 aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc ccgtagaaaa
     6421 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6541 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6601 gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6661 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6721 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6781 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6841 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6901 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6961 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7021 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7081 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7141 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7201 ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc tgacggatgg
     7261 cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt cttaagctcg
     7321 ggccccctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg
     7381 cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag
     7441 ggcgcctgtc actttgcttg atatatgaga attatttaac cttataaatg agaaaaaagc
     7501 aacgcacttt aaataagata cgttgctttt tcgattgatg aacacctata attaaactat
     7561 tcatctatta tttatgattt tttgtatata caatatttct agtttgttaa agagaattaa
     7621 gaaaataaat ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa attatacatg
     7681 tcaacgataa tacaaaatat aatacaaact ataagatgtt atcagtattt attatgcatt
     7741 tagaataaat tttgtgtcgc ccttattcga ctcactatag aagttcctat tctctagaaa
     7801 gtataggaac ttcacttcat tttatttaaa tttgcggccg c
//
