LOCUS       Exported                8095 bp ds-DNA     circular SYN 28-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2134..2150
                     /label=M13R
                     /note="M13R"
     misc_feature    2136..2154
                     /label=M13 reverse priming site
                     /note="M13 reverse priming site"
     misc_feature    2177..2180
                     /label=Pme1 site
                     /note="Pme1 site"
     misc_feature    2185..2392
                     /label=5' homology arm
                     /note="5' homology arm"
     enhancer        2406..2783
                     /label=hCMV enhancer
                     /note="hCMV enhancer"
     misc_feature    complement(2729..2750)
                     /label=HN853
                     /note="HN853"
     promoter        2789..4130
                     /label=AG hybrid promoter
                     /note="AG hybrid promoter"
     misc_feature    2789..3066
                     /label=Chicken b-actin promoter
                     /note="Chicken b-actin promoter"
     misc_feature    2976..2980
                     /label=CCATT box
                     /note="CCATT box"
     misc_feature    3038..3045
                     /label=TATA box
                     /note="TATA box"
     misc_feature    3050..3066
                     /label=TSS
                     /note="TSS"
     misc_feature    3067..3157
                     /label=Chicken b-actin exon 1
                     /note="Chicken b-actin exon 1"
     misc_feature    3158..4082
                     /label=Chicken b-actin rabbit globin hybrid
                     /note="Chicken b-actin rabbit globin hybrid intron"
     intron          3158..4033
                     /label=Chicken b-actin intron
                     /note="Chicken b-actin intron"
     misc_feature    4034..4130
                     /label=Rabbit b-globin splice acceptor
                     /note="Rabbit b-globin splice acceptor"
     misc_feature    4047..4067
                     /label=HN737
                     /note="HN737"
     misc_feature    4091..4110
                     /label=HN382
                     /note="HN382"
     gene            complement(4142..4175)
                     /label=loxP
                     /note="loxP"
     misc_feature    4182..4185
                     /label=Untitled
     misc_feature    4202..4429
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    4435..4662
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    4668..4895
                     /label=SV40 pA
                     /note="SV40 pA"
     gene            complement(4918..4951)
                     /label=loxP
                     /note="loxP"
     misc_feature    4961..6391
                     /label=tdTomato
                     /note="tdTomato"
     misc_feature    4961..4981
                     /label=Untitled
     misc_feature    4961..4981
                     /label=HN921
                     /note="HN921"
     misc_feature    5624..5707
                     /label=linker
                     /note="linker"
     misc_feature    complement(5628..5645)
                     /label=Untitled
     misc_feature    5653
                     /label=c to t Age1
                     /note="c to t Age1"
     misc_feature    complement(6368..6391)
                     /label=Untitled
     misc_feature    complement(6370..6391)
                     /label=HN304
                     /note="HN304"
     misc_feature    6400..6985
                     /label=WPRE
     misc_feature    7006..7307
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    7324..7437
                     /label=3' homology arm
                     /note="3' homology arm"
     misc_feature    complement(7450..7471)
                     /label=T7 priming site
                     /note="T7 priming site"
     misc_feature    complement(7480..7496)
                     /label=M13 F2 priming site
                     /note="M13 F2 priming site"
     misc_feature    complement(7480..7496)
                     /label=M13F
                     /note="M13F"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaagttt aaacaagggc aggatgtgtc aaactgccaa tagagaacta
     2221 cttactcttc aggctgaagc tgatggaaca ggtaacaaag gcaaacacta atcatgatca
     2281 gcaagatgaa gcagaaaggg aacaagggga tattaaatgt gtatagacac gctagagaga
     2341 tggctcagca gttaagagaa ctagctggtc tttcagaggt cctgagatca ataccggtca
     2401 tggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     2461 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2521 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2581 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2641 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2701 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2761 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2821 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     2881 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
     2941 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     3001 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     3061 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
     3121 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3181 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3241 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     3301 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     3361 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     3421 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     3481 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     3541 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     3601 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     3661 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
     3721 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     3781 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     3841 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     3901 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     3961 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     4021 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     4081 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgctag
     4141 cataacttcg tatagcatac attatacgaa gttatagcca ccgcggtggc ggccgctcta
     4201 gaatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     4261 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     4321 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4381 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ctagaatcat
     4441 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4501 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4621 gcattctagt tgtggtttgt ccaaactcat caatgtatct taactagaat cataatcagc
     4681 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4741 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4801 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4861 agttgtggtt tgtccaaact catcaatgta tcttaactag tggatctcat gcctaggata
     4921 acttcgtata gcatacatta tacgaagtta taggcgcgcc atggtgagca agggcgagga
     4981 ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
     5041 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     5101 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
     5161 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     5221 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     5281 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     5341 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     5401 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     5461 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     5521 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     5581 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg
     5641 gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa
     5701 catggccgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
     5761 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     5821 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     5881 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
     5941 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
     6001 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
     6061 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
     6121 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
     6181 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
     6241 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
     6301 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
     6361 cctgtacggc atggacgagc tgtacaagta attaattaaa acctctggat tacaaaattt
     6421 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6481 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6541 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6601 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6661 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6721 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6781 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6841 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6901 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6961 tctccctttg ggccgcctcc ccgcaggatc cactagagcg gccgcctaga gctcgctgat
     7021 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7081 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7141 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7201 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     7261 aggcggaaag aaccagctgg ggctcgacta gagcttgcgg aacccttaag cttgtcattc
     7321 gaatttagac acccacatgg tggctcatga ccatctatct ataaatggat ctgattttca
     7381 tgtctggcag tgtacagaag ctaactgaag aaaggtggaa gacccacaag agttcaaggt
     7441 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     7501 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     7561 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     7621 cctgaatggc gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     7681 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     7741 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     7801 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     7861 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     7921 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     7981 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     8041 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcag
//
