LOCUS       Exported                3068 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. """Minimal cargo"" vector.
            Allows introducing any part using BsmBI; but doesn't have cloning
            reporter. Can be used to generate new promoter/terminator acceptor
            vectors.".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    61..81
                     /label="Main module - minimal 2"
                     /note="'Minimal' main modular cloning site 2. To generate
                     any level 2 (or 0) acceptor plasmid. BsmBI overlap with
                     truncated BsaI site, BsmBI cloning enables reconstructing
                     BsaI site with any fusion site."
     misc_feature    82..102
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    103..126
                     /label="Downstream Module"
     terminator      complement(132..234)
                     /label="T0 terminator"
     primer_bind     190..206
                     /label="PS3"
     primer_bind     complement(190..206)
                     /label="PS2"
     CDS             395..1255
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     1326..1343
                     /label="PS5"
     misc_feature    complement(1328..1532)
                     /label="oriT"
     primer_bind     complement(1533..1550)
                     /label="PS4"
     rep_origin      1573..2192
                     /label="pBR322_origin"
     misc_feature    complement(2178..2180)
                     /label="ORI"
     terminator      complement(2963..3067)
                     /label="T1 terminator"
     primer_bind     complement(2966..2983)
                     /label="PS6"
     primer_bind     2966..2983
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtcttgaga cgtctctgac ctactagtag cggccgctgc agggagttgt cttcgaagac
      121 ttcgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      181 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
      241 ccaataatta cgatttaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc
      301 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      361 ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc
      421 gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt
      481 gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct
      541 gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac
      601 ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact
      661 gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa
      721 acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga
      781 taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt
      841 tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga
      901 agcgattccg aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg
      961 taaactgctg acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat
     1021 ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat
     1081 tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc
     1141 ggatggtaaa ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga
     1201 tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga
     1261 tacaattaaa ggctcctttt ggagcctttt tttttggacg acccttgtcc ttttccgctg
     1321 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
     1381 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
     1441 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
     1501 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg
     1561 gccccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1621 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1681 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1741 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1801 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1861 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1921 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1981 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2041 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2101 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2161 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2221 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2281 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2341 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2401 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2461 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     2521 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     2581 gacagtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     2641 gcagctgcgg taaagctcat cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc
     2701 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     2761 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
     2821 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     2881 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     2941 gcggcggggg cgcgcccagc tgtctagggc ggcggatttg tcctactcag gagagcgttc
     3001 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     3061 tgatgcct
//
