LOCUS       Exported                3911 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Terminator acceptor vector.
            Minimal cargo vector; with eGFP in downstream secondary site and
            constitutive promoter J23100 in upstream site. Allows introducing
            any part using BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..76
                     /label="Upstream Module"
     promoter        13..48
                     /label="J23100"
     misc_feature    101..122
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    123..143
                     /label="LVL-2 Main Module_minimal"
     misc_feature    144..164
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    165..969
                     /label="Downstream Module"
     CDS             229..939
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(975..1077)
                     /label="T0 terminator"
     primer_bind     1033..1049
                     /label="PS3"
     primer_bind     complement(1033..1049)
                     /label="PS2"
     CDS             1238..2098
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2169..2186
                     /label="PS5"
     misc_feature    complement(2171..2375)
                     /label="oriT"
     primer_bind     complement(2376..2393)
                     /label="PS4"
     rep_origin      2416..3035
                     /label="pBR322_origin"
     primer_bind     complement(2999..3009)
                     /label="PS6"
     terminator      complement(3806..3890)
                     /label="T1 terminator"
     primer_bind     complement(3809..3826)
                     /label="PS6"
     primer_bind     3809..3826
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtcttga gacgtctctg acctactagt agcggccgct gcagggagta acttctgact
      181 gagttgcata cttgtttaac tttaagaagg agatatacaa tggtagcccg taaaggagaa
      241 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      301 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      361 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
      421 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
      481 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      541 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      601 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      661 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
      721 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
      781 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
      841 gccctttcga aagatcccaa cgaaaagaga gatcacatgg tccttcttga gtttgtaaca
      901 gctgctggga ttacacatgg catggatgaa ctatacaaag gttcgtaact tctgactgag
      961 ttgcacgctc tagtcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     1021 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccagggg
     1081 tccccaataa ttacgattta aattagtagc ccgcctaatg agcgggcttt tttttaattc
     1141 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1201 ctgataaatg cttcaataat attgaaaaag gaagagtatg agcattcagc attttcgtgt
     1261 ggcgctgatt ccgttttttg cggcgttttg cctgccggtg tttgcgcatc cggaaaccct
     1321 ggtgaaagtg aaagatgcgg aagatcaact gggtgcgcgc gtgggctata ttgaactgga
     1381 tctgaacagc ggcaaaattc tggaatcttt tcgtccggaa gaacgttttc cgatgatgag
     1441 cacctttaaa gtgctgctgt gcggtgcggt tctgagccgt gtggatgcgg gccaggaaca
     1501 actgggccgt cgtattcatt atagccagaa cgatctggtg gaatatagcc cggtgaccga
     1561 aaaacatctg accgatggca tgaccgtgcg tgaactgtgc agcgcggcga ttaccatgag
     1621 cgataacacc gcggcgaacc tgctgctgac gaccattggc ggtccgaaag aactgaccgc
     1681 gtttctgcat aacatgggcg atcatgtgac ccgtctggat cgttgggaac cggaactgaa
     1741 cgaagcgatt ccgaacgatg aacgtgatac caccatgccg gcagcaatgg cgaccaccct
     1801 gcgtaaactg ctgacgggtg agctgctgac cctggcaagc cgccagcaac tgattgattg
     1861 gatggaagcg gataaagtgg cgggtccgct gctgcgtagc gcgctgccgg ctggctggtt
     1921 tattgcggat aaaagcggtg cgggcgaacg tggcagccgt ggcattattg cggcgctggg
     1981 cccggatggt aaaccgagcc gtattgtggt gatttatacc accggcagcc aggcgacgat
     2041 ggatgaacgt aaccgtcaga ttgcggaaat tggcgcgagc ctgattaaac attggtaaac
     2101 cgatacaatt aaaggctcct tttggagcct ttttttttgg acgacccttg tccttttccg
     2161 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     2221 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     2281 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     2341 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg
     2401 ccggccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2461 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2521 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2581 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2641 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2701 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2761 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2821 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2881 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2941 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3001 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3061 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3181 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     3241 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     3301 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3421 tgtgacagtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3481 gaggcagctg cggtaaagct catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg
     3541 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     3601 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     3661 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     3721 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     3781 gatgcggcgg gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg
     3841 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     3901 atttgatgcc t
//
