LOCUS       Exported                3862 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Level 2 vector. OriV 9
            (pBBR322/ROP; medium copy number); sfGFP cloning reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    61..925
                     /label="LVL-2 Main Module_minimal"
     RBS             126..146
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             163..873
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label="superfolder GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    926..946
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    947..970
                     /label="Downstream Module"
     terminator      complement(976..1078)
                     /label="T0 terminator"
     primer_bind     complement(1034..1050)
                     /label="PS2"
     primer_bind     1034..1050
                     /label="PS3"
     CDS             1302..2093
                     /label="spectinomycin/streptomycin resistance protein"
     misc_feature    complement(2122..2326)
                     /label="OriT"
     rep_origin      2367..2986
                     /label="pBR322_origin"
     primer_bind     complement(2950..2960)
                     /label="PS6"
     misc_feature    complement(2972..2974)
                     /label="ORI"
     terminator      complement(3757..3861)
                     /label="T1 terminator"
     primer_bind     3760..3777
                     /label="PS1"
     primer_bind     complement(3760..3777)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctctaat gggagtgaga cgggagttga cggctagctc agtcctaggt acagtgctag
      121 cgtacttgtt taactttaag aaggagatat acaatggtag cccgtaaagg cgaagagctg
      181 ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt
      241 tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc
      301 tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt
      361 gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc
      421 atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa
      481 acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc
      541 attgacttta aagaagatgg caatatcctg ggccataagc tggaatacaa ttttaacagc
      601 cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt
      661 cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca
      721 atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg
      781 tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg
      841 ggcatcacgc atggtatgga tgaactgtac aaaggttcgt aacttctgac tgagttgcac
      901 gctcgtctct cgctagcctg agacctacta gtagcggccg ctgcagggag ttgtcttcga
      961 agacttcgct ctagtcttgg actcctgttg atagatccag taatgacctc agaactccat
     1021 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg
     1081 gtccccaata attacgattt aaatgaacct tgaccgaacg cagcggtggt aacggcgcag
     1141 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     1201 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1261 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     1321 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     1381 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     1441 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     1501 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     1561 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     1621 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     1681 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     1741 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     1801 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggtgatgag cgaaatgtag
     1861 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     1921 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     1981 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2041 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taagacaatt
     2101 gtccttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc
     2161 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
     2221 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
     2281 cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg
     2341 ctggccgtag gccggccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2401 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2461 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2521 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2581 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2641 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2701 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2761 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2821 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2881 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2941 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3001 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3061 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3121 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     3181 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     3241 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     3301 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3361 tacagacaag ctgtgacagt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3421 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag
     3481 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     3541 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     3601 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
     3661 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     3721 ctggcggtat ggatgcggcg ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac
     3781 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     3841 cctttcgttt tatttgatgc ct
//
