LOCUS       Exported                3890 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Level 1 vector. sfGFP
            reporter. Thermosensitive origin pSC101 (low copy number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..46
                     /label="Upstream Module"
     misc_feature    47..68
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             167..877
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label="superfolder GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    930..950
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    951..974
                     /label="Downstream Module"
     terminator      complement(980..1082)
                     /label="T0 terminator"
     primer_bind     complement(1038..1054)
                     /label="PS2"
     primer_bind     1038..1054
                     /label="PS3"
     CDS             1210..2025
                     /label="Km"
     primer_bind     2073..2090
                     /label="PS5"
     rep_origin      complement(2075..2279)
                     /direction=LEFT
                     /label="oriT"
     primer_bind     complement(2280..2297)
                     /label="PS4"
     rep_origin      complement(2311..3261)
                     /direction=LEFT
                     /label="origin pSC101"
                     /note="origin pSC101"
     misc_feature    complement(3094..3096)
                     /product="Ala>Val"
                     /label="Ala>Val"
                     /note="Ala>Val mutation turns oriV thermosensitive"
     misc_feature    3265..3770
                     /label="ori"
                     /note="ori"
     terminator      complement(3785..3889)
                     /label="T1 terminator"
     primer_bind     3788..3805
                     /label="PS1"
     primer_bind     complement(3788..3805)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctggagt gagaccggag ttgacggcta gctcagtcct aggtacagtg
      121 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagcccgta aaggcgaaga
      181 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
      241 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
      301 catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta
      361 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
      421 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
      481 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
      541 aggcattgac tttaaagaag atggcaatat cctgggccat aagctggaat acaattttaa
      601 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
      661 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
      721 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
      781 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
      841 agcgggcatc acgcatggta tggatgaact gtacaaaggt tcgtaacttc tgactgagtt
      901 gcacgctggt ctctcgctaa tgtgagacgt actagtagcg gccgctgcag ggagttgtct
      961 tcgaagactt cgctctagtc ttggactcct gttgatagat ccagtaatga cctcagaact
     1021 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
     1081 aggggtcccc aataattacg atttaaattt gtgtctcaaa atctctgatg ttacattgca
     1141 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1201 aggggtgtta tgagccatat tcagcgtgaa acgagctgta gccgtccgcg tctgaacagc
     1261 aacatggatg cggatctgta tggctataaa tgggcgcgtg ataacgtggg tcagagcggc
     1321 gcgaccattt atcgtctgta tggcaaaccg gatgcgccgg aactgtttct gaaacatggc
     1381 aaaggcagcg tggcgaacga tgtgaccgat gaaatggtgc gtctgaactg gctgaccgaa
     1441 tttatgccgc tgccgaccat taaacatttt attcgcaccc cggatgatgc gtggctgctg
     1501 accaccgcga ttccgggcaa aaccgcgttt caggtgctgg aagaatatcc ggatagcggc
     1561 gaaaacattg tggatgcgct ggccgtgttt ctgcgtcgtc tgcatagcat tccggtgtgc
     1621 aactgcccgt ttaacagcga tcgtgtgttt cgtctggccc aggcgcagag ccgtatgaac
     1681 aacggcctgg tggatgcgag cgattttgat gatgaacgta acggctggcc ggtggaacag
     1741 gtgtggaaag aaatgcataa actgctgccg tttagcccgg atagcgtggt gacccacggc
     1801 gattttagcc tggataacct gattttcgat gaaggcaaac tgattggctg cattgatgtg
     1861 ggccgtgtgg gcattgcgga tcgttatcag gatctggcca ttctgtggaa ctgcctgggc
     1921 gaatttagcc cgagcctgca aaaacgtctg tttcagaaat atggcattga taatccggat
     1981 atgaacaaac tgcaatttca tctgatgctg gatgaatttt tctaataatt aattggaccg
     2041 cggtccgcgc gttgtccttt tccgctgcat aaccctgctt cggggtcatt atagcgattt
     2101 tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag
     2161 actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg
     2221 cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc
     2281 ctgctctgcg aggctggccg taggccggcc tcagatcctt ccgtatttag ccagtatgtt
     2341 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt
     2401 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
     2461 agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt
     2521 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
     2581 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
     2641 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
     2701 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
     2761 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
     2821 ctcataaatc ctaatagagt atttgttttc aaaagactta acatgttcca gattatattt
     2881 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
     2941 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa
     3001 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
     3061 ataagcattt tccctactga tgttcatcat ctgaacgtat tggttataag tgaacgatac
     3121 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
     3181 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
     3241 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
     3301 attgagatgg gctagtcaat gataattaca tgtccttttc ctttgagttg tgggtatctg
     3361 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc
     3421 cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa
     3481 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta
     3541 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag
     3601 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat
     3661 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg
     3721 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc ggcgcgccca
     3781 gctgtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     3841 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
//
