LOCUS       Exported                3446 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Level 1 vector. With lacZ as
            alternative cloning reporter. Thermosensitive origin pSC101 (low
            copy number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..46
                     /label="Upstream Module"
     misc_feature    47..68
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             167..433
                     /codon_start=1
                     /label="lacZ"
                     /translation="GDPKLLLEVPHAISSSPGNSLAVVLQRRDWENPGVTQLNRLAAHP
                     PFASWRNSEEARTDRPSQQLRSLIRLLTKPERKLSWLLPPLSNN"
     misc_feature    486..506
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    507..530
                     /label="Downstream Module"
     terminator      complement(536..638)
                     /label="T0 terminator"
     primer_bind     complement(594..610)
                     /label="PS2"
     primer_bind     594..610
                     /label="PS3"
     CDS             766..1581
                     /label="Km"
     primer_bind     1629..1646
                     /label="PS5"
     rep_origin      complement(1631..1835)
                     /direction=LEFT
                     /label="oriT"
     primer_bind     complement(1836..1853)
                     /label="PS4"
     rep_origin      complement(1867..2817)
                     /direction=LEFT
                     /label="origin pSC101"
                     /note="origin pSC101"
     misc_feature    complement(2650..2652)
                     /product="Ala>Val"
                     /label="Ala>Val"
                     /note="Ala>Val mutation turns oriV thermosensitive"
     misc_feature    2821..3326
                     /label="ori"
                     /note="ori"
     terminator      complement(3341..3445)
                     /label="T1 terminator"
     primer_bind     3344..3361
                     /label="PS1"
     primer_bind     complement(3344..3361)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctggagt gagaccggag ttgacggcta gctcagtcct aggtacagtg
      121 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagccggtg acccgaaact
      181 gctgctggaa gttccgcacg ctatctcttc ttctccgggt aactctctgg ctgttgttct
      241 gcaacgtcgt gactgggaaa acccgggtgt tacccagctg aaccgtctgg ctgctcaccc
      301 gccgttcgct tcttggcgta actctgaaga agctcgtacc gaccgtccgt cccagcagct
      361 gcgttctctg atccgtctgc tgaccaaacc ggaacgtaaa ctgtcttggc tgctgccgcc
      421 gctgtctaac aacggttcgt aacttctgac tgagttgcac gctggtctct cgctaatgtg
      481 agacgtacta gtagcggccg ctgcagggag ttgtcttcga agacttcgct ctagtcttgg
      541 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
      601 cggttgccgc cgggcgtttt ttattggtga gaatccaggg gtccccaata attacgattt
      661 aaatttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      721 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcag
      781 cgtgaaacga gctgtagccg tccgcgtctg aacagcaaca tggatgcgga tctgtatggc
      841 tataaatggg cgcgtgataa cgtgggtcag agcggcgcga ccatttatcg tctgtatggc
      901 aaaccggatg cgccggaact gtttctgaaa catggcaaag gcagcgtggc gaacgatgtg
      961 accgatgaaa tggtgcgtct gaactggctg accgaattta tgccgctgcc gaccattaaa
     1021 cattttattc gcaccccgga tgatgcgtgg ctgctgacca ccgcgattcc gggcaaaacc
     1081 gcgtttcagg tgctggaaga atatccggat agcggcgaaa acattgtgga tgcgctggcc
     1141 gtgtttctgc gtcgtctgca tagcattccg gtgtgcaact gcccgtttaa cagcgatcgt
     1201 gtgtttcgtc tggcccaggc gcagagccgt atgaacaacg gcctggtgga tgcgagcgat
     1261 tttgatgatg aacgtaacgg ctggccggtg gaacaggtgt ggaaagaaat gcataaactg
     1321 ctgccgttta gcccggatag cgtggtgacc cacggcgatt ttagcctgga taacctgatt
     1381 ttcgatgaag gcaaactgat tggctgcatt gatgtgggcc gtgtgggcat tgcggatcgt
     1441 tatcaggatc tggccattct gtggaactgc ctgggcgaat ttagcccgag cctgcaaaaa
     1501 cgtctgtttc agaaatatgg cattgataat ccggatatga acaaactgca atttcatctg
     1561 atgctggatg aatttttcta ataattaatt ggaccgcggt ccgcgcgttg tccttttccg
     1621 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     1681 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     1741 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     1801 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg
     1861 ccggcctcag atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg
     1921 cgtgagccat gagaacgaac cattgagatc atacttactt tgcatgtcac tcaaaaattt
     1981 tgcctcaaaa ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag
     2041 tccgttatgt aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt
     2101 tatctggttg ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa
     2161 aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt
     2221 gtttaaatct ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta
     2281 gttattttca agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt
     2341 gtgagttttc ttttgtgtta gttcttttaa taaccactca taaatcctaa tagagtattt
     2401 gttttcaaaa gacttaacat gttccagatt atattttatg aattttttta actggaaaag
     2461 ataaggcaat atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata
     2521 gtttgtccac tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct
     2581 cgtcatcagc tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt
     2641 catcatctga acgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt
     2701 ttcaatcgtg gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt
     2761 taagtcatag cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca
     2821 tctcaattgg tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata
     2881 attacatgtc cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg
     2941 aaaacttgta aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt
     3001 tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag
     3061 aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag
     3121 gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag
     3181 tagcaccctc gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc
     3241 acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga
     3301 atgggggtaa atggcactac aggcgcggcg cgcccagctg tctagggcgg cggatttgtc
     3361 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     3421 tgagcctttc gttttatttg atgcct
//
