LOCUS       Exported                3263 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Level 1 vector with
            alternative selection marker (chloramphenicol). Origin pUC (high
            copy number). sfGFP cloning reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
            .
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..46
                     /label="Upstream Module"
     misc_feature    47..68
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             167..877
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label="superfolder GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    930..950
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    951..974
                     /label="Downstream Module"
     terminator      complement(980..1082)
                     /label="T0 terminator"
     primer_bind     1038..1054
                     /label="PS3"
     primer_bind     complement(1038..1054)
                     /label="PS2"
     CDS             1212..1862
                     /codon_start=1
                     /product="cat"
                     /label="cat"
                     /note="derived from S. aureus pC194"
                     /protein_id="AID49098.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     primer_bind     1977..1994
                     /label="PS5"
     oriT            complement(1979..2183)
                     /direction=LEFT
                     /label="oriT"
     primer_bind     complement(2184..2201)
                     /label="PS4"
     rep_origin      complement(2245..3112)
                     /direction=LEFT
                     /label="pUC Ori"
     primer_bind     complement(2807..2817)
                     /label="PS6"
     terminator      complement(3158..3262)
                     /label="T1 terminator"
     primer_bind     complement(3161..3178)
                     /label="PS6"
     primer_bind     3161..3178
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctggagt gagaccggag ttgacggcta gctcagtcct aggtacagtg
      121 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagcccgta aaggcgaaga
      181 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
      241 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
      301 catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta
      361 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
      421 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
      481 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
      541 aggcattgac tttaaagaag atggcaatat cctgggccat aagctggaat acaattttaa
      601 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
      661 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
      721 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
      781 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
      841 agcgggcatc acgcatggta tggatgaact gtacaaaggt tcgtaacttc tgactgagtt
      901 gcacgctggt ctctcgctaa tgtgagacgt actagtagcg gccgctgcag ggagttgtct
      961 tcgaagactt cgctctagtc ttggactcct gttgatagat ccagtaatga cctcagaact
     1021 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
     1081 aggggtcccc aataattacg atttaaataa caaacgggat tgacttttaa aaaaggattg
     1141 attctaatga agaaagcaga caagtaagcc tcctaaattc actttagata aaaatttagg
     1201 aggcatatca aatgaacttt aataaaattg atttagacaa ttggaagaga aaagagatat
     1261 ttaatcatta tttgaaccaa caaacgactt ttagtataac cacagaaatt gatattagtg
     1321 ttttataccg aaacataaaa caagaaggat ataaatttta ccctgcattt attttcttag
     1381 tgacaagggt gataaactca aatacagctt ttagaactgg ttacaatagc gacggagagt
     1441 taggttattg ggataagtta gagccacttt atacaatttt tgatggtgta tctaaaacat
     1501 tctctggtat ttggactcct gtaaagaatg acttcaaaga gttttatgat ttataccttt
     1561 ctgatgtaga gaaatataat ggttcgggga aattgtttcc caaaacacct atacctgaaa
     1621 atgctttttc tctttctatt attccatgga cttcatttac tgggtttaac ttaaatatca
     1681 ataataatag taattacctt ctacccatta ttacagcagg aaaattcatt aataaaggta
     1741 attcaatata tttaccgcta tctttacagg tacatcattc tgtttgtgat ggttatcatg
     1801 caggattgtt tatgaactct attcaggaat tgtcagatag gcctaatgac tggcttttat
     1861 aatatgagat aatgccgact gtacttttta cagtcggttt tctaatgtca ctagggctcg
     1921 cctttgggaa gtttgaaggg acttcggtcc gcgcgttgtc cttttccgct gcataaccct
     1981 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
     2041 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
     2101 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
     2161 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag
     2221 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2341 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctttgagaaa
     2641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2761 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2821 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2881 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2941 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3001 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3061 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     3121 tgagttagct cactcattag gcaggcgcgc ccagctgtct agggcggcgg atttgtccta
     3181 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
     3241 gcctttcgtt ttatttgatg cct
//
