LOCUS       Exported                4639 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Level 2 vector. sfGFP
            reporter. Origin RK2 (broad-host-range).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     source          4376
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    61..921
                     /label="LVL-2 Main Module_minimal"
     RBS             122..142
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..869
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label="superfolder GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    922..942
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    943..966
                     /label="Downstream Module"
     terminator      complement(972..1074)
                     /label="T0 terminator"
     primer_bind     complement(1030..1046)
                     /label="PS2"
     primer_bind     1030..1046
                     /label="PS3"
     CDS             1298..2089
                     /label="spectinomycin/streptomycin resistance protein"
     primer_bind     2116..2133
                     /label="PS5"
     misc_feature    complement(2118..2322)
                     /label="OriT"
     primer_bind     complement(2323..2340)
                     /label="PS4"
     gene            complement(2404..3552)
                     /gene="trfA"
                     /label="trfA"
     CDS             complement(2404..3552)
                     /codon_start=1
                     /gene="trfA"
                     /product="replication protein"
                     /label="trfA"
                     /protein_id="AFV59806.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4376
                     /label="Deletion in parental pSEVA521"
                     /note="Parental pSEVA521 was described to have sequence
                     'acgccgcgccgggtgagtcggcccctcaagtgtcaacgtccgcccctcatctgtca'
                     in this position, but not present when sequenced."
     terminator      complement(4534..4638)
                     /label="T1 terminator"
     primer_bind     4537..4554
                     /label="PS1"
     primer_bind     complement(4537..4554)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctctgga gtgagacggg agttgacggc tagctcagtc ctaggtacag tgctagcgta
      121 cttgtttaac tttaagaagg agatatacaa tggtagcccg taaaggcgaa gagctgttca
      181 ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg
      241 tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta
      301 ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc
      361 agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc
      421 cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc
      481 gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg
      541 actttaaaga agatggcaat atcctgggcc ataagctgga atacaatttt aacagccaca
      601 atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc
      661 acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg
      721 gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta
      781 aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca
      841 tcacgcatgg tatggatgaa ctgtacaaag gttcgtaact tctgactgag ttgcacgctc
      901 gtctctcgct aatgtgagac ctactagtag cggccgctgc agggagttgt cttcgaagac
      961 ttcgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
     1021 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
     1081 ccaataatta cgatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1141 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     1201 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1261 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     1321 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1381 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     1441 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1501 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1561 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1621 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1681 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     1741 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     1801 aaccttaacg ctatggaact cgccgcccga ctgggctggt gatgagcgaa atgtagtgct
     1861 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1921 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1981 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2041 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcc
     2101 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     2161 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     2221 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     2281 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     2341 ccgtaggccg gccgcgatgc aggtggctgc tgaaccccca gccggaactg accccacaag
     2401 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct
     2461 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga
     2521 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga
     2581 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat
     2641 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg
     2701 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga
     2761 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag
     2821 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag
     2881 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac
     2941 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt
     3001 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt
     3061 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc
     3121 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg
     3181 tttcagcaac gcggcctgct tggcttcgct gacctgtttt gccaggtcct cgccggcggt
     3241 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc
     3301 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc
     3361 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggactatcga
     3421 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc
     3481 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa
     3541 cgtccgattc attcaccctc cttgcgggat tgccccggaa ttaattcccc ggatcgatcc
     3601 gtcgatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     3661 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     3721 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     3781 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
     3841 cagcaccgtt tctgcggact ggctttctac gtggctgcca tttttggggt gaggccgttc
     3901 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
     3961 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
     4021 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
     4081 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
     4141 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
     4201 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
     4261 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
     4321 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcagtga
     4381 gggccaagtt ttccgcgagg tatccacaac gccggcggcc ctacatggct ctgctgtagt
     4441 gagtgggttg cgctccggca gcggtcctga tcccccgcag aaaaaaagga tctcaagaag
     4501 atcctttgat cttttctacg gcgcgcccag ctgtctaggg cggcggattt gtcctactca
     4561 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
     4621 ttcgttttat ttgatgcct
//
