LOCUS       Exported                3995 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Level 2 vector. sfGFP
            reporter. Origin pBBR1 (medium-copy; broad-host-range).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    61..921
                     /label="LVL-2 Main Module_minimal"
     RBS             122..142
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..869
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label="superfolder GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    922..942
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    943..966
                     /label="Downstream Module"
     terminator      complement(972..1074)
                     /label="T0 terminator"
     primer_bind     complement(1030..1046)
                     /label="PS2"
     primer_bind     1030..1046
                     /label="PS3"
     CDS             1298..2089
                     /label="spectinomycin/streptomycin resistance protein"
     primer_bind     2116..2133
                     /label="PS5"
     misc_feature    complement(2118..2322)
                     /label="OriT"
     primer_bind     complement(2323..2340)
                     /label="PS4"
     gene            complement(2354..3016)
                     /gene="rep"
                     /label="rep"
     CDS             complement(2354..3016)
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label="rep"
                     /protein_id="AFV59764.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     terminator      complement(3890..3994)
                     /label="T1 terminator"
     primer_bind     3893..3910
                     /label="PS1"
     primer_bind     complement(3893..3910)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctctgga gtgagacggg agttgacggc tagctcagtc ctaggtacag tgctagcgta
      121 cttgtttaac tttaagaagg agatatacaa tggtagcccg taaaggcgaa gagctgttca
      181 ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg
      241 tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta
      301 ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc
      361 agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc
      421 cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc
      481 gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg
      541 actttaaaga agatggcaat atcctgggcc ataagctgga atacaatttt aacagccaca
      601 atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc
      661 acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg
      721 gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta
      781 aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca
      841 tcacgcatgg tatggatgaa ctgtacaaag gttcgtaact tctgactgag ttgcacgctc
      901 gtctctcgct aatgtgagac ctactagtag cggccgctgc agggagttgt cttcgaagac
      961 ttcgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
     1021 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
     1081 ccaataatta cgatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1141 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     1201 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1261 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     1321 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1381 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     1441 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1501 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1561 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1621 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1681 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     1741 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     1801 aaccttaacg ctatggaact cgccgcccga ctgggctggt gatgagcgaa atgtagtgct
     1861 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1921 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1981 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2041 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcc
     2101 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     2161 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     2221 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     2281 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     2341 ccgtaggccg gccctaccgg cgcggcagcg ttacccgtgt cggcggctcc aacggctcgc
     2401 catcgtccag aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc
     2461 attcctccgt ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc
     2521 tgggcggctc ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga
     2581 tgcggcgcag gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca
     2641 cggcggcact gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt
     2701 cattgaccac gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc
     2761 gctcggccac caagtccttg actgcgtatt ggaccgtccg caaagaacgt ccgatgagct
     2821 tggaaagtgt tttctggctg accaccacgg cgttctggtg gcccatctgc gccacgaggt
     2881 gatgcagcag cattgccgcc gtgggtttcc tcgcaataag cccggcccac gcctcatgcg
     2941 ctttgcgttc cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc
     3001 tggactgcgt ggccatgctt atctccatgc ggtaggggtg ccgcacggtt gcggcaccat
     3061 gcgcaatcag ctgcaacttt tcggcagcgc gacaacaatt atgcgttgcg taaaagtggc
     3121 agtcaattac agattttctt taacctacgc aatgagctat tgcggggggt gccgcaatga
     3181 gctgttgcgt accccccttt tttaagttgt tgatttttaa gtctttcgca tttcgcccta
     3241 tatctagttc tttggtgccc aaagaagggc acccctgcgg ggttccccca cgccttcggc
     3301 gcggctcccc ctccggcaaa aagtggcccc tccggggctt gttgatcgac tgcgcggcct
     3361 tcggccttgc ccaaggtggc gctgccccct tggaaccccc gcactcgccg ccgtgaggct
     3421 cggggggcag gcgggcgggc ttcgcccttc gactgccccc actcgcatag gcttgggtcg
     3481 ttccaggcgc gtcaaggcca agccgctgcg cggtcgctgc gcgagccttg acccgccttc
     3541 cacttggtgt ccaaccggca agcgaagcgc gcaggccgca ggccggaggc ttttccccag
     3601 agaaaattaa aaaaattgat ggggcaaggc cgcaggccgc gcagttggag ccggtgggta
     3661 tgtggtcgaa ggctgggtag ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc
     3721 agcctgtcca tcagcttgtc cagcagggtt gtccacgggc cgagcgaagc gagccagccg
     3781 gtggccgctc gcggccatcg tccacatatc cacgggctgg caagggagcg cagcgaccgc
     3841 gcagggcgaa gcccggagag caagcccgta gggggggcgc gcccagctgt ctagggcggc
     3901 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     3961 tctttcgact gagcctttcg ttttatttga tgcct
//
