LOCUS       Exported                4442 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Level 2 vector. sfGFP
            reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    61..921
                     /label="LVL-2 Main Module_minimal"
     RBS             122..142
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..869
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label="superfolder GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    922..942
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    943..966
                     /label="Downstream Module"
     terminator      complement(972..1074)
                     /label="T0 terminator"
     primer_bind     complement(1030..1046)
                     /label="PS2"
     primer_bind     1030..1046
                     /label="PS3"
     CDS             1298..2089
                     /label="spectinomycin/streptomycin resistance protein"
     primer_bind     2116..2133
                     /label="PS5"
     misc_feature    complement(2118..2322)
                     /label="OriT"
     primer_bind     complement(2323..2340)
                     /label="PS4"
     primer_bind     complement(3004..3014)
                     /label="PS6"
     gene            complement(3079..3921)
                     /gene="rep"
                     /label="rep"
     CDS             complement(3079..3921)
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label="rep"
                     /protein_id="AFV59777.1"
                     /translation="MSNVASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVT
                     DEVMYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGW
                     VQTIIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWD
                     SIGQREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKV
                     THLLFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     CDS             complement(3079..3912)
                     /codon_start=1
                     /product="replication protein for the broad-host-range
                     plasmid pRO1600 from Pseudomonas aeruginosa"
                     /label="pRO1600 Rep"
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3926..4277
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(4337..4441)
                     /label="T1 terminator"
     primer_bind     4340..4357
                     /label="PS1"
     primer_bind     complement(4340..4357)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctctgga gtgagacggg agttgacggc tagctcagtc ctaggtacag tgctagcgta
      121 cttgtttaac tttaagaagg agatatacaa tggtagcccg taaaggcgaa gagctgttca
      181 ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg
      241 tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta
      301 ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc
      361 agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc
      421 cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc
      481 gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg
      541 actttaaaga agatggcaat atcctgggcc ataagctgga atacaatttt aacagccaca
      601 atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc
      661 acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg
      721 gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta
      781 aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca
      841 tcacgcatgg tatggatgaa ctgtacaaag gttcgtaact tctgactgag ttgcacgctc
      901 gtctctcgct aatgtgagac ctactagtag cggccgctgc agggagttgt cttcgaagac
      961 ttcgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
     1021 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
     1081 ccaataatta cgatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1141 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     1201 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1261 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     1321 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1381 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     1441 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1501 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1561 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1621 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1681 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     1741 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     1801 aaccttaacg ctatggaact cgccgcccga ctgggctggt gatgagcgaa atgtagtgct
     1861 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1921 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1981 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2041 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcc
     2101 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     2161 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     2221 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     2281 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     2341 ccgtaggccg gccgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2401 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2461 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2521 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2581 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2641 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2701 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2761 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2821 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggcatccggt
     2881 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2941 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3001 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gccgtgaaag gcaggccggt
     3061 ccgtggtggc cacggcctct aggccagatc cagcggcatc tgggttagtc gagcgcgggc
     3121 cgcttcccat gtctcaccag ggcgagcctg tttcgcgatc tcagcatctg aaatcttccc
     3181 ggccttgcgc ttcgctgggg ccttacccac cgccttggcg ggcttcttcg gtccaaaact
     3241 gaacaacaga tgtgtgacct tgcgcccggt ctttcgctgc gcccactcca cctgtagcgg
     3301 gctgtgctcg ttgatctgcg tcacggctgg atcaagcact cgcaacttga agtccttgat
     3361 cgagggatac cggccttcca gttgaaacca ctttcgcagc tggtcaattt ctatttcgcg
     3421 ctggccgatg ctgtcccatt gcatgagcag ctcgtaaagc ctgatcgcgt gggtgctgtc
     3481 catcttggcc acgtcagcca aggcgtattt ggtgaactgt ttggtgagtt ccgtcaggta
     3541 cggcagcatg tctttggtga acctgagttc tacacggccc tcaccctccc ggtagatgat
     3601 tgtttgcacc cagccggtaa tcatcacact cggtcttttc cccttgccat tgggctcttg
     3661 ggttaaccgg acttcccgcc gtttcaggcg cagggccgct tctttgagct ggttgtagga
     3721 agattcgata gggacacccg ccatcgtcgc tatgtcctcc gccgtcactg aatacatcac
     3781 ttcatcggtg acaggctcgc tcctcttcac ctggctaata caggccagaa cgatccgctg
     3841 ttcctgaaca ctgaggcgat acgcggcctc gaccagggca ttgcttttgt aaaccattgg
     3901 gggtgaggcc acgttcgaca ttccttgtgt ataaggggac actgtatctg cgtcccacaa
     3961 tacaacaaat ccgtcccttt acaacaacaa atccgtccct tcttaacaac aaatccgtcc
     4021 cttaatggca acaaatccgt ccctttttaa actctacagg ccacggatta cgtggcctgt
     4081 agacgtccta aaaggtttaa aagggaaaag gaagaaaagg gtggaaacgc aaaaaacgca
     4141 ccactacgtg gccccgttgg ggccgcattt gtgcccctga aggggcgggg gaggcgtctg
     4201 ggcaatcccc gttttaccag tcccctatcg ccgcctgaga gggcgcagga agcgagtaat
     4261 cagggtatcg aggcggattc acccttggcg tccaaccagc ggcaccagcg gcgcctgaga
     4321 ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac
     4381 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     4441 ct
//
