LOCUS       Exported                3206 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Level 2 vector. sfGFP
            reporter. Origin p15A (medium copy number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    61..921
                     /label="LVL-2 Main Module_minimal"
     RBS             122..142
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             159..869
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label="superfolder GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    922..942
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    943..966
                     /label="Downstream Module"
     terminator      complement(972..1074)
                     /label="T0 terminator"
     primer_bind     complement(1030..1046)
                     /label="PS2"
     primer_bind     1030..1046
                     /label="PS3"
     CDS             1298..2089
                     /label="spectinomycin/streptomycin resistance protein"
     primer_bind     2116..2133
                     /label="PS5"
     misc_feature    complement(2118..2322)
                     /label="OriT"
     primer_bind     complement(2323..2340)
                     /label="PS4"
     rep_origin      2354..3086
                     /label="p15A ori"
                     /note="p15A ori"
     primer_bind     complement(2914..2924)
                     /label="PS6"
     terminator      complement(3101..3205)
                     /label="T1 terminator"
     primer_bind     3104..3121
                     /label="PS1"
     primer_bind     complement(3104..3121)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctctgga gtgagacggg agttgacggc tagctcagtc ctaggtacag tgctagcgta
      121 cttgtttaac tttaagaagg agatatacaa tggtagcccg taaaggcgaa gagctgttca
      181 ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg
      241 tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta
      301 ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc
      361 agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc
      421 cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc
      481 gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg
      541 actttaaaga agatggcaat atcctgggcc ataagctgga atacaatttt aacagccaca
      601 atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc
      661 acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg
      721 gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta
      781 aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca
      841 tcacgcatgg tatggatgaa ctgtacaaag gttcgtaact tctgactgag ttgcacgctc
      901 gtctctcgct aatgtgagac ctactagtag cggccgctgc agggagttgt cttcgaagac
      961 ttcgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
     1021 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
     1081 ccaataatta cgatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1141 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     1201 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1261 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     1321 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1381 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     1441 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1501 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1561 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1621 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1681 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     1741 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     1801 aaccttaacg ctatggaact cgccgcccga ctgggctggt gatgagcgaa atgtagtgct
     1861 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1921 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1981 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2041 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcc
     2101 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     2161 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     2221 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     2281 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     2341 ccgtaggccg gccctagaaa tattttatct gattaataag atgatcttct tgagatcgtt
     2401 ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt
     2461 cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt
     2521 caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct
     2581 ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac
     2641 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata
     2701 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa
     2761 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     2821 gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
     2881 ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
     2941 cttttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc
     3001 tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc
     3061 agtgagcgag gaagcggaat atatccggcg cgcccagctg tctagggcgg cggatttgtc
     3121 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     3181 tgagcctttc gttttatttg atgcct
//
