LOCUS       Exported                3912 bp    ds-DNA  circular SYN 04-MAY-2018
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part P- Promoter
            (constitutive); J23103..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    86..120
                     /label="J23103"
     misc_feature    145..165
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    166..970
                     /label="Downstream Module"
     CDS             230..940
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(976..1078)
                     /label="T0 terminator"
     primer_bind     1034..1050
                     /label="PS3"
     primer_bind     complement(1034..1050)
                     /label="PS2"
     CDS             1239..2099
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2170..2187
                     /label="PS5"
     misc_feature    complement(2172..2376)
                     /label="oriT"
     primer_bind     complement(2377..2394)
                     /label="PS4"
     rep_origin      2417..3036
                     /label="pBR322_origin"
     primer_bind     complement(3000..3010)
                     /label="PS6"
     misc_feature    complement(3022..3024)
                     /label="ORI"
     terminator      complement(3807..3891)
                     /label="T1 terminator"
     primer_bind     complement(3810..3827)
                     /label="PS6"
     primer_bind     3810..3827
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagctgat agctagctca gtcctaggga ttatgctagc
      121 tacttgagac ctttcatcgc gacctactag tagcggccgc tgcagggagt aacttctgac
      181 tgagttgcat acttgtttaa ctttaagaag gagatataca atggtagccc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agatcacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa ggttcgtaac ttctgactga
      961 gttgcacgct ctagtcttgg actcctgttg atagatccag taatgacctc agaactccat
     1021 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg
     1081 gtccccaata attacgattt aaattagtag cccgcctaat gagcgggctt ttttttaatt
     1141 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1201 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg
     1261 tggcgctgat tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc
     1321 tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg
     1381 atctgaacag cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga
     1441 gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac
     1501 aactgggccg tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg
     1561 aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga
     1621 gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg
     1681 cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga
     1741 acgaagcgat tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc
     1801 tgcgtaaact gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt
     1861 ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt
     1921 ttattgcgga taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg
     1981 gcccggatgg taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga
     2041 tggatgaacg taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa
     2101 ccgatacaat taaaggctcc ttttggagcc tttttttttg gacgaccctt gtccttttcc
     2161 gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc
     2221 gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg
     2281 cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact
     2341 gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag
     2401 gccggccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2461 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2521 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2581 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2641 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2701 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2761 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2821 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2881 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2941 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3001 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3061 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3121 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3181 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     3241 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3301 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     3361 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     3421 ctgtgacagt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     3481 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag atgtctgcct
     3541 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     3601 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     3661 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     3721 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     3781 ggatgcggcg ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc
     3841 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     3901 tatttgatgc ct
//
