LOCUS       Exported                3908 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part P- Promoter
            (constitutive); J23119 (1bp short on 3?)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     promoter        86..116
                     /label="J23119 short"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119), 1 bp shorter on 3' end site"
     misc_feature    109..114
                     /label="-10"
     misc_feature    141..161
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    162..966
                     /label="Downstream Module"
     CDS             226..936
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(972..1074)
                     /label="T0 terminator"
     primer_bind     1030..1046
                     /label="PS3"
     primer_bind     complement(1030..1046)
                     /label="PS2"
     CDS             1235..2095
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2166..2183
                     /label="PS5"
     misc_feature    complement(2168..2372)
                     /label="oriT"
     primer_bind     complement(2373..2390)
                     /label="PS4"
     rep_origin      2413..3032
                     /label="pBR322_origin"
     primer_bind     complement(2996..3006)
                     /label="PS6"
     misc_feature    complement(3018..3020)
                     /label="ORI"
     terminator      complement(3803..3887)
                     /label="T1 terminator"
     primer_bind     complement(3806..3823)
                     /label="PS6"
     primer_bind     3806..3823
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagttgac agctagctca gtcctaggta taatgctact
      121 tgagaccttt catcgcgacc tactagtagc ggccgctgca gggagtaact tctgactgag
      181 ttgcatactt gtttaacttt aagaaggaga tatacaatgg tagcccgtaa aggagaagaa
      241 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      301 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      361 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
      421 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
      481 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      541 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      601 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
      661 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
      721 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
      781 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
      841 ctttcgaaag atcccaacga aaagagagat cacatggtcc ttcttgagtt tgtaacagct
      901 gctgggatta cacatggcat ggatgaacta tacaaaggtt cgtaacttct gactgagttg
      961 cacgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
     1021 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
     1081 ccaataatta cgatttaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc
     1141 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1201 ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc
     1261 gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt
     1321 gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct
     1381 gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac
     1441 ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact
     1501 gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa
     1561 acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga
     1621 taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt
     1681 tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga
     1741 agcgattccg aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg
     1801 taaactgctg acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat
     1861 ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat
     1921 tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc
     1981 ggatggtaaa ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga
     2041 tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga
     2101 tacaattaaa ggctcctttt ggagcctttt tttttggacg acccttgtcc ttttccgctg
     2161 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
     2221 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
     2281 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
     2341 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg
     2401 gccccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3061 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3121 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3181 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3241 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     3301 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     3361 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3421 gacagtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3481 gcagctgcgg taaagctcat cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc
     3541 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     3601 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
     3661 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     3721 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     3781 gcggcggggg cgcgcccagc tgtctagggc ggcggatttg tcctactcag gagagcgttc
     3841 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     3901 tgatgcct
//
