LOCUS       Exported                4007 bp    ds-DNA  circular SYN 21-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. "Basic Part P- Promoter
            (non-constitutive); Pbad (I13453).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    86..215
                     /label="I13453 (Pbad)"
                     /label="I13453"
                     /note="Pbad promoter from iGEM collection, part I13453"
     misc_feature    240..260
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    261..1065
                     /label="Downstream Module"
     CDS             325..1035
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1071..1173)
                     /label="T0 terminator"
     primer_bind     1129..1145
                     /label="PS3"
     primer_bind     complement(1129..1145)
                     /label="PS2"
     CDS             1334..2194
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2265..2282
                     /label="PS5"
     misc_feature    complement(2267..2471)
                     /label="oriT"
     primer_bind     complement(2472..2489)
                     /label="PS4"
     rep_origin      2512..3131
                     /label="pBR322_origin"
     primer_bind     complement(3095..3105)
                     /label="PS6"
     misc_feature    complement(3117..3119)
                     /label="ORI"
     terminator      complement(3902..3986)
                     /label="T1 terminator"
     primer_bind     complement(3905..3922)
                     /label="PS6"
     primer_bind     3905..3922
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagacatt gattatttgc acggcgtcac actttgctat
      121 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
      181 tctactgttt ctccataccg tttttttggg ctagctactt gagacctttc atcgcgacct
      241 actagtagcg gccgctgcag ggagtaactt ctgactgagt tgcatacttg tttaacttta
      301 agaaggagat atacaatggt agcccgtaaa ggagaagaac ttttcactgg agttgtccca
      361 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      421 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      481 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      541 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      601 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      661 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      721 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      781 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      841 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      901 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      961 aagagagatc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1021 gatgaactat acaaaggttc gtaacttctg actgagttgc acgctctagt cttggactcc
     1081 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     1141 gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt
     1201 agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat
     1261 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1321 aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc
     1381 gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga
     1441 tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga
     1501 atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg
     1561 tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag
     1621 ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac
     1681 cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct
     1741 gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca
     1801 tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg
     1861 tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct
     1921 gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg
     1981 tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg
     2041 cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat
     2101 tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc
     2161 ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg
     2221 gagccttttt ttttggacga cccttgtcct tttccgctgc ataaccctgc ttcggggtca
     2281 ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa
     2341 gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag
     2401 taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc
     2461 tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa aagatcaaag
     2521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2641 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3301 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     3361 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     3421 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     3481 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acagtctccg ggagctgcat
     3541 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     3601 agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     3661 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     3721 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     3781 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     3841 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggggc gcgcccagct
     3901 gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     3961 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//
