LOCUS       Exported                3926 bp    ds-DNA  circular SYN 10-JAN-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part P- Promoter
            (non-constitutive); Pci; controlled by cI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     promoter        86..134
                     /label="Pci"
     misc_feature    159..179
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    180..984
                     /label="Downstream Module"
     CDS             244..954
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(990..1092)
                     /label="T0 terminator"
     primer_bind     1048..1064
                     /label="PS3"
     primer_bind     complement(1048..1064)
                     /label="PS2"
     CDS             1253..2113
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2184..2201
                     /label="PS5"
     misc_feature    complement(2186..2390)
                     /label="oriT"
     primer_bind     complement(2391..2408)
                     /label="PS4"
     rep_origin      2431..3050
                     /label="pBR322_origin"
     primer_bind     complement(3014..3024)
                     /label="PS6"
     misc_feature    complement(3036..3038)
                     /label="ORI"
     terminator      complement(3821..3905)
                     /label="T1 terminator"
     primer_bind     complement(3824..3841)
                     /label="PS6"
     primer_bind     3824..3841
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagtaaca ccgtgcgtgt tgactatttt acctctggcg
      121 gtgataatgg ttgctacttg agacctttca tcgcgaccta ctagtagcgg ccgctgcagg
      181 gagtaacttc tgactgagtt gcatacttgt ttaactttaa gaaggagata tacaatggta
      241 gcccgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      301 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      361 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      421 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      481 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      541 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      601 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      661 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      721 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      781 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      841 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagatca catggtcctt
      901 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaaggttcg
      961 taacttctga ctgagttgca cgctctagtc ttggactcct gttgatagat ccagtaatga
     1021 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     1081 gtgagaatcc aggggtcccc aataattacg atttaaatta gtagcccgcc taatgagcgg
     1141 gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1201 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagcat
     1261 tcagcatttt cgtgtggcgc tgattccgtt ttttgcggcg ttttgcctgc cggtgtttgc
     1321 gcatccggaa accctggtga aagtgaaaga tgcggaagat caactgggtg cgcgcgtggg
     1381 ctatattgaa ctggatctga acagcggcaa aattctggaa tcttttcgtc cggaagaacg
     1441 ttttccgatg atgagcacct ttaaagtgct gctgtgcggt gcggttctga gccgtgtgga
     1501 tgcgggccag gaacaactgg gccgtcgtat tcattatagc cagaacgatc tggtggaata
     1561 tagcccggtg accgaaaaac atctgaccga tggcatgacc gtgcgtgaac tgtgcagcgc
     1621 ggcgattacc atgagcgata acaccgcggc gaacctgctg ctgacgacca ttggcggtcc
     1681 gaaagaactg accgcgtttc tgcataacat gggcgatcat gtgacccgtc tggatcgttg
     1741 ggaaccggaa ctgaacgaag cgattccgaa cgatgaacgt gataccacca tgccggcagc
     1801 aatggcgacc accctgcgta aactgctgac gggtgagctg ctgaccctgg caagccgcca
     1861 gcaactgatt gattggatgg aagcggataa agtggcgggt ccgctgctgc gtagcgcgct
     1921 gccggctggc tggtttattg cggataaaag cggtgcgggc gaacgtggca gccgtggcat
     1981 tattgcggcg ctgggcccgg atggtaaacc gagccgtatt gtggtgattt ataccaccgg
     2041 cagccaggcg acgatggatg aacgtaaccg tcagattgcg gaaattggcg cgagcctgat
     2101 taaacattgg taaaccgata caattaaagg ctccttttgg agcctttttt tttggacgac
     2161 ccttgtcctt ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat
     2221 atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt
     2281 ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt
     2341 gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc
     2401 gaggctggcc gtaggccggc cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2581 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3061 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3121 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3181 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     3241 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     3301 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     3361 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3421 cgcttacaga caagctgtga cagtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3481 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc
     3541 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     3601 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     3661 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     3721 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     3781 acaactggcg gtatggatgc ggcgggggcg cgcccagctg tctagggcgg cggatttgtc
     3841 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     3901 tgagcctttc gttttatttg atgcct
//
