LOCUS       Exported                3947 bp    ds-DNA  circular SYN 17-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part P- Promoter
            (non-constitutive); Pars; metal sensitive; controlled by arsR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     promoter        86..155
                     /label="Pars"
     misc_feature    180..200
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    201..1005
                     /label="Downstream Module"
     CDS             265..975
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1011..1113)
                     /label="T0 terminator"
     primer_bind     1069..1085
                     /label="PS3"
     primer_bind     complement(1069..1085)
                     /label="PS2"
     CDS             1274..2134
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2205..2222
                     /label="PS5"
     misc_feature    complement(2207..2411)
                     /label="oriT"
     primer_bind     complement(2412..2429)
                     /label="PS4"
     rep_origin      2452..3071
                     /label="pBR322_origin"
     primer_bind     complement(3035..3045)
                     /label="PS6"
     misc_feature    complement(3057..3059)
                     /label="ORI"
     terminator      complement(3842..3926)
                     /label="T1 terminator"
     primer_bind     complement(3845..3862)
                     /label="PS6"
     primer_bind     3845..3862
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggaggtata tacacattcg ttaagtcata tatgtttttg
      121 acttatccgc ttcgaagaga gacactacct gcaactactt gagacctttc atcgcgacct
      181 actagtagcg gccgctgcag ggagtaactt ctgactgagt tgcatacttg tttaacttta
      241 agaaggagat atacaatggt agcccgtaaa ggagaagaac ttttcactgg agttgtccca
      301 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      361 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      421 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      481 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      541 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      601 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      661 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      721 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      781 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      841 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      901 aagagagatc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      961 gatgaactat acaaaggttc gtaacttctg actgagttgc acgctctagt cttggactcc
     1021 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     1081 gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt
     1141 agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat
     1201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1261 aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc
     1321 gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga
     1381 tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga
     1441 atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg
     1501 tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag
     1561 ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac
     1621 cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct
     1681 gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca
     1741 tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg
     1801 tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct
     1861 gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg
     1921 tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg
     1981 cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat
     2041 tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc
     2101 ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg
     2161 gagccttttt ttttggacga cccttgtcct tttccgctgc ataaccctgc ttcggggtca
     2221 ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa
     2281 gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag
     2341 taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc
     2401 tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa aagatcaaag
     2461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2581 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3061 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3241 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     3301 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     3361 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     3421 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acagtctccg ggagctgcat
     3481 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     3541 agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     3601 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     3661 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     3721 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     3781 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggggc gcgcccagct
     3841 gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     3901 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//
