LOCUS       Exported                3937 bp    ds-DNA  circular SYN 17-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part P- Promoter
            (non-constitutive); Pmer; metal sensitive; controlled by merR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     promoter        86..145
                     /label="Pmer"
     misc_feature    170..190
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    191..995
                     /label="Downstream Module"
     CDS             255..965
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1001..1103)
                     /label="T0 terminator"
     primer_bind     1059..1075
                     /label="PS3"
     primer_bind     complement(1059..1075)
                     /label="PS2"
     CDS             1264..2124
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2195..2212
                     /label="PS5"
     misc_feature    complement(2197..2401)
                     /label="oriT"
     primer_bind     complement(2402..2419)
                     /label="PS4"
     rep_origin      2442..3061
                     /label="pBR322_origin"
     primer_bind     complement(3025..3035)
                     /label="PS6"
     misc_feature    complement(3047..3049)
                     /label="ORI"
     terminator      complement(3832..3916)
                     /label="T1 terminator"
     primer_bind     complement(3835..3852)
                     /label="PS6"
     primer_bind     3835..3852
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagatcgc ttgactccgt acatgagtac ggaagtaagg
      121 ttacgctatc caatttcaat tcgaatactt gagacctttc atcgcgacct actagtagcg
      181 gccgctgcag ggagtaactt ctgactgagt tgcatacttg tttaacttta agaaggagat
      241 atacaatggt agcccgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      301 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      361 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      421 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      481 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      541 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      601 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      661 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      721 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      781 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      841 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagatc
      901 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
      961 acaaaggttc gtaacttctg actgagttgc acgctctagt cttggactcc tgttgataga
     1021 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
     1081 gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc
     1141 ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat
     1201 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1261 agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg
     1321 ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt
     1381 gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga atcttttcgt
     1441 ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg
     1501 agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat
     1561 ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa
     1621 ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc
     1681 attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt
     1741 ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc
     1801 atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg
     1861 gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg
     1921 cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc
     1981 agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt
     2041 tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc
     2101 gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt
     2161 ttttggacga cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
     2221 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
     2281 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
     2341 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
     2401 tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa aagatcaaag gatcttcttg
     2461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2581 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     3241 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     3301 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     3361 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3421 ctcccggcat ccgcttacag acaagctgtg acagtctccg ggagctgcat gtgtcagagg
     3481 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     3541 tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     3601 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     3661 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     3721 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     3781 tgtgagggta aacaactggc ggtatggatg cggcgggggc gcgcccagct gtctagggcg
     3841 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3901 agtctttcga ctgagccttt cgttttattt gatgcct
//
