LOCUS       Exported                2672 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part RN-
            RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus
            start..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     source          85..111
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     RBS             85..111
                     /label="RBS-pET"
                     /note="Efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             113..118
                     /codon_start=1
                     /label="MV"
                     /note="Methionine-Valine translational start. Valine codon
                     GUA following AUG inititiation codon has strong translation
                     initiation in Escherichia coli (Looman et al, 1987. PMID:
                     3311730) "
                     /translation="MV"
     misc_feature    142..162
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    163..186
                     /label="Downstream Module"
     terminator      complement(192..294)
                     /label="T0 terminator"
     primer_bind     complement(250..266)
                     /label="PS2"
     primer_bind     250..266
                     /label="PS3"
     CDS             455..1315
                     /label="AmpR"
     primer_bind     1386..1403
                     /label="PS5"
     misc_feature    complement(1388..1592)
                     /label="OriT"
     primer_bind     complement(1593..1610)
                     /label="PS4"
     rep_origin      complement(1654..2521)
                     /direction=LEFT
                     /label="pUC Ori"
     primer_bind     complement(2216..2226)
                     /label="PS6"
     terminator      complement(2567..2671)
                     /label="T1 terminator"
     primer_bind     2570..2587
                     /label="PS1"
     primer_bind     complement(2570..2587)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc ttacttgttt aactttaaga aggagatata caatggtagc
      121 ctgagacctt tcatcgcgac ctactagtag cggccgctgc agggagttgt cttcgaagac
      181 ttcgctctag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      241 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
      301 ccaataatta cgatttaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc
      361 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      421 ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc
      481 gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt
      541 gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct
      601 gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac
      661 ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact
      721 gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa
      781 acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga
      841 taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt
      901 tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga
      961 agcgattccg aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg
     1021 taaactgctg acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat
     1081 ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat
     1141 tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc
     1201 ggatggtaaa ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga
     1261 tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga
     1321 tacaattaaa ggctcctttt ggagcctttt tttttggacg acccttgtcc ttttccgctg
     1381 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
     1441 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
     1501 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
     1561 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg
     1621 gccccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1681 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1741 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1801 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1861 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1921 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1981 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2041 tttgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2101 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2161 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2221 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2281 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2341 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2401 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2461 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2521 caattaatgt gagttagctc actcattagg caggcgcgcc cagctgtcta gggcggcgga
     2581 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     2641 ttcgactgag cctttcgttt tatttgatgc ct
//
