LOCUS       Exported                2666 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part RN-
            RBS+N-terminus; B0034m Ribosome Binding Site + Met-Val N-terminus
            start..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     5'UTR           86..105
                     /label="B0033m"
     CDS             107..112
                     /codon_start=1
                     /label="MV"
                     /note="Methionine-Valine translational start. Valine codon
                     GUA following AUG inititiation codon has strong translation
                     initiation in Escherichia coli (Looman et al, 1987. PMID:
                     3311730) "
                     /translation="MV"
     misc_feature    136..156
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    157..180
                     /label="Downstream Module"
     terminator      complement(186..288)
                     /label="T0 terminator"
     primer_bind     complement(244..260)
                     /label="PS2"
     primer_bind     244..260
                     /label="PS3"
     CDS             449..1309
                     /label="AmpR"
     primer_bind     1380..1397
                     /label="PS5"
     misc_feature    complement(1382..1586)
                     /label="OriT"
     primer_bind     complement(1587..1604)
                     /label="PS4"
     rep_origin      complement(1648..2515)
                     /direction=LEFT
                     /label="pUC Ori"
     primer_bind     complement(2210..2220)
                     /label="PS6"
     terminator      complement(2561..2665)
                     /label="T1 terminator"
     primer_bind     2564..2581
                     /label="PS1"
     primer_bind     complement(2564..2581)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc ttactagagt cacacaggac tactaaatgg tagcctgaga
      121 cctttcatcg cgacctacta gtagcggccg ctgcagggag ttgtcttcga agacttcgct
      181 ctagtcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
      241 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg gtccccaata
      301 attacgattt aaattagtag cccgcctaat gagcgggctt ttttttaatt cccctatttg
      361 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      421 gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg tggcgctgat
      481 tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc tggtgaaagt
      541 gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg atctgaacag
      601 cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga gcacctttaa
      661 agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac aactgggccg
      721 tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg aaaaacatct
      781 gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga gcgataacac
      841 cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg cgtttctgca
      901 taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga acgaagcgat
      961 tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc tgcgtaaact
     1021 gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt ggatggaagc
     1081 ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt ttattgcgga
     1141 taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg gcccggatgg
     1201 taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga tggatgaacg
     1261 taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa ccgatacaat
     1321 taaaggctcc ttttggagcc tttttttttg gacgaccctt gtccttttcc gctgcataac
     1381 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata
     1441 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg
     1501 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt
     1561 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccgtag gccggccccg
     1621 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1681 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1741 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1801 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1861 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1921 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1981 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctttgag
     2041 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2101 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2161 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2221 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2281 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2341 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2401 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2461 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2521 atgtgagtta gctcactcat taggcaggcg cgcccagctg tctagggcgg cggatttgtc
     2581 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     2641 tgagcctttc gttttatttg atgcct
//
