LOCUS       Exported                2667 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part RN-
            RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val
            N-terminus start..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     RBS             86..106
                     /label="B0034m"
     CDS             108..113
                     /codon_start=1
                     /label="MV"
                     /note="Methionine-Valine translational start. Valine codon
                     GUA following AUG inititiation codon has strong translation
                     initiation in Escherichia coli (Looman et al, 1987. PMID:
                     3311730) "
                     /translation="MV"
     misc_feature    137..157
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    158..181
                     /label="Downstream Module"
     terminator      complement(187..289)
                     /label="T0 terminator"
     primer_bind     complement(245..261)
                     /label="PS2"
     primer_bind     245..261
                     /label="PS3"
     CDS             450..1310
                     /label="AmpR"
     primer_bind     1381..1398
                     /label="PS5"
     misc_feature    complement(1383..1587)
                     /label="OriT"
     primer_bind     complement(1588..1605)
                     /label="PS4"
     rep_origin      complement(1649..2516)
                     /direction=LEFT
                     /label="pUC Ori"
     primer_bind     complement(2211..2221)
                     /label="PS6"
     terminator      complement(2562..2666)
                     /label="T1 terminator"
     primer_bind     2565..2582
                     /label="PS1"
     primer_bind     complement(2565..2582)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc ttactagaga aagaggagaa atactaaatg gtagcctgag
      121 acctttcatc gcgacctact agtagcggcc gctgcaggga gttgtcttcg aagacttcgc
      181 tctagtcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
      241 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccagg ggtccccaat
      301 aattacgatt taaattagta gcccgcctaa tgagcgggct tttttttaat tcccctattt
      361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      421 tgcttcaata atattgaaaa aggaagagta tgagcattca gcattttcgt gtggcgctga
      481 ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca tccggaaacc ctggtgaaag
      541 tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta tattgaactg gatctgaaca
      601 gcggcaaaat tctggaatct tttcgtccgg aagaacgttt tccgatgatg agcaccttta
      661 aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc gggccaggaa caactgggcc
      721 gtcgtattca ttatagccag aacgatctgg tggaatatag cccggtgacc gaaaaacatc
      781 tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc gattaccatg agcgataaca
      841 ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa agaactgacc gcgtttctgc
      901 ataacatggg cgatcatgtg acccgtctgg atcgttggga accggaactg aacgaagcga
      961 ttccgaacga tgaacgtgat accaccatgc cggcagcaat ggcgaccacc ctgcgtaaac
     1021 tgctgacggg tgagctgctg accctggcaa gccgccagca actgattgat tggatggaag
     1081 cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc ggctggctgg tttattgcgg
     1141 ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat tgcggcgctg ggcccggatg
     1201 gtaaaccgag ccgtattgtg gtgatttata ccaccggcag ccaggcgacg atggatgaac
     1261 gtaaccgtca gattgcggaa attggcgcga gcctgattaa acattggtaa accgatacaa
     1321 ttaaaggctc cttttggagc cttttttttt ggacgaccct tgtccttttc cgctgcataa
     1381 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     1441 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     1501 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     1561 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggcccc
     1621 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1681 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1741 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1801 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1861 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1921 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1981 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctttga
     2041 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2101 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2161 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2221 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2281 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2341 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2401 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2461 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2521 aatgtgagtt agctcactca ttaggcaggc gcgcccagct gtctagggcg gcggatttgt
     2581 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
     2641 ctgagccttt cgttttattt gatgcct
//
