LOCUS       Exported                3719 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part O- ORF; lacI;
            transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
            .
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..56
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     CDS             86..1162
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label="lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="KPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELN
                     YIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVE
                     ACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHE
                     DGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAM
                     SGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCY
                     IPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRA
                     LADSLMQLARQVSRLESGQ"
     misc_feature    1189..1209
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    1210..1233
                     /label="Downstream Module"
     terminator      complement(1239..1341)
                     /label="T0 terminator"
     primer_bind     complement(1297..1313)
                     /label="PS2"
     primer_bind     1297..1313
                     /label="PS3"
     CDS             1502..2362
                     /label="AmpR"
     primer_bind     2433..2450
                     /label="PS5"
     misc_feature    complement(2435..2639)
                     /label="OriT"
     primer_bind     complement(2640..2657)
                     /label="PS4"
     rep_origin      complement(2701..3568)
                     /direction=LEFT
                     /label="pUC Ori"
     primer_bind     complement(3263..3273)
                     /label="PS6"
     terminator      complement(3614..3718)
                     /label="T1 terminator"
     primer_bind     complement(3617..3634)
                     /label="PS6"
     primer_bind     3617..3634
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagccaaacc agtaacgtta tacgatgtcg cagagtatgc
      121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      181 aacgcgggaa aaagtggaag cggctatggc ggagctgaat tacattccca accgcgtggc
      241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
      361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
      421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
      481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
      541 gacacccatc aacagtatta ttttctccca tgaagatggt acgcgactgg gcgtggagca
      601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
      661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
      721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
      781 gaatgagggc atcgttccca ctgctatgct ggttgccaac gatcagatgg cgctgggcgc
      841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
      901 cgacgatacc gaagatagct catgttatat cccgccgtta accaccatca aacaggattt
      961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1021 gaagggcaat cagctgttgc ccgtgtcact ggtgaaaaga aaaaccaccc tggcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc agggttcgtg agacctttca tcgcgaccta ctagtagcgg
     1201 ccgctgcagg gagttgtctt cgaagacttc gctctagtct tggactcctg ttgatagatc
     1261 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
     1321 tttttattgg tgagaatcca ggggtcccca ataattacga tttaaattag tagcccgcct
     1381 aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac attcaaatat
     1441 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1501 tatgagcatt cagcattttc gtgtggcgct gattccgttt tttgcggcgt tttgcctgcc
     1561 ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat gcggaagatc aactgggtgc
     1621 gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa attctggaat cttttcgtcc
     1681 ggaagaacgt tttccgatga tgagcacctt taaagtgctg ctgtgcggtg cggttctgag
     1741 ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt cattatagcc agaacgatct
     1801 ggtggaatat agcccggtga ccgaaaaaca tctgaccgat ggcatgaccg tgcgtgaact
     1861 gtgcagcgcg gcgattacca tgagcgataa caccgcggcg aacctgctgc tgacgaccat
     1921 tggcggtccg aaagaactga ccgcgtttct gcataacatg ggcgatcatg tgacccgtct
     1981 ggatcgttgg gaaccggaac tgaacgaagc gattccgaac gatgaacgtg ataccaccat
     2041 gccggcagca atggcgacca ccctgcgtaa actgctgacg ggtgagctgc tgaccctggc
     2101 aagccgccag caactgattg attggatgga agcggataaa gtggcgggtc cgctgctgcg
     2161 tagcgcgctg ccggctggct ggtttattgc ggataaaagc ggtgcgggcg aacgtggcag
     2221 ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg agccgtattg tggtgattta
     2281 taccaccggc agccaggcga cgatggatga acgtaaccgt cagattgcgg aaattggcgc
     2341 gagcctgatt aaacattggt aaaccgatac aattaaaggc tccttttgga gccttttttt
     2401 ttggacgacc cttgtccttt tccgctgcat aaccctgctt cggggtcatt atagcgattt
     2461 tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag
     2521 actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg
     2581 cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc
     2641 ctgctctgcg aggctggccg taggccggcc ccgtagaaaa gatcaaagga tcttcttgag
     2701 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2761 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2821 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2881 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2941 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3001 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3061 ccgaactgag atacctacag cgtgagcttt gagaaagcgc cacgcttccc gaagggagaa
     3121 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3181 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3241 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3301 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3361 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3421 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3481 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3541 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcag
     3601 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
     3661 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
