LOCUS       Exported                3041 bp ds-DNA     circular SYN 26-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part O- ORF; cueR; 
            transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Marcos Valenzuela-Ortega, Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic 
            biology
  JOURNAL   Publication in preparation
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 26, 2019 from SnapGene 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label=Upstream Module
     misc_feature    39..56
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ATG"
     CDS             86..486
                     /codon_start=1
                     /gene="cueR"
                     /gene_synonym="ECK0481; JW0476; ybbI"
                     /locus_tag="b0487"
                     /product="copper-responsive regulon transcriptional 
                     regulator"
                     /function="putative transport; Not classified"
                     /label=cueR
                     /note="GO_function=GO:0016564 - transcription repressor 
                     activity GO_process=GO:0006350 - transcription"
                     /db_xref="ASAP:ABE-0001693"
                     /db_xref="UniProtKB/Swiss-Prot:P0A9G4"
                     /db_xref="EcoGene:EG13256"
                     /db_xref="GeneID:945332"
                     /protein_id="NP_415020.1"
                     /translation="NIRDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHLNE
                     LTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMRDQL
                     LALANACPGDDSADCPIIENLSGCCHHRA"
     misc_feature    511..531
                     /label=BioBrick suffix
                     /note="BioBrick universal suffix for all parts"
     misc_feature    532..555
                     /label=Downstream Module
     terminator      complement(561..663)
                     /label=T0 terminator
     primer_bind     complement(619..635)
                     /label=PS2
     primer_bind     619..635
                     /label=PS3
     CDS             824..1684
                     /label=AmpR
     primer_bind     1755..1772
                     /label=PS5
     misc_feature    complement(1757..1961)
                     /label=OriT
     primer_bind     complement(1962..1979)
                     /label=PS4
     rep_origin      complement(2023..2890)
                     /direction=LEFT
                     /label=pUC Ori
     primer_bind     complement(2585..2595)
                     /label=PS6
     terminator      complement(2936..3040)
                     /label=T1 terminator
     primer_bind     complement(2939..2956)
                     /label=PS6
     primer_bind     2939..2956
                     /label=PS1
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagccaacat cagggatgta gcaaaaatta ccggcctgac
      121 cagcaaagcc attcgcttct atgaagagaa ggggctggtg acgccgccga tgcgcagcga
      181 aaacggttat cgcacctaca cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc
      241 acggcaggtt ggctttaacc tggaagagag cggcgagctg gtgaatctgt ttaacgaccc
      301 gcagcggcac agcgccgacg tcaaacggcg cacgctggag aaggtggcgg agatcgaacg
      361 acacattgag gagctgcaat ccatgcgcga ccagctgctg gcactggcga atgcctgccc
      421 tggggatgac agcgccgact gcccgattat cgaaaatctc tccggctgct gtcatcatcg
      481 ggcaggttcg tgagaccttt catcgcgacc tactagtagc ggccgctgca gggagttgtc
      541 ttcgaagact tcgctctagt cttggactcc tgttgataga tccagtaatg acctcagaac
      601 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
      661 caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg ggcttttttt
      721 taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      781 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca ttcagcattt
      841 tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg cgcatccgga
      901 aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg gctatattga
      961 actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac gttttccgat
     1021 gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca
     1081 ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat atagcccggt
     1141 gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac
     1201 catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc cgaaagaact
     1261 gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga
     1321 actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag caatggcgac
     1381 caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc agcaactgat
     1441 tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg
     1501 ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca ttattgcggc
     1561 gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg gcagccaggc
     1621 gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga ttaaacattg
     1681 gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga cccttgtcct
     1741 tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct
     1801 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
     1861 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
     1921 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
     1981 cgtaggccgg ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2401 agcgtgagct ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2761 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2821 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2881 agcgcaacgc aattaatgtg agttagctca ctcattaggc aggcgcgccc agctgtctag
     2941 ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     3001 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc t
//

YKUnique 6+ CuttersHOdEÿÿÿÿYô?