LOCUS       Exported                4522 bp    ds-DNA  circular SYN 19-MAR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. "Basic Part NOC- Complete
            ORF; LambdaRED / ?RED; Policistronic ""NOC"" part with coding
            regions for the three lambda red proteins (the first; gam; doesn't
            have RBS; but bet and exo genes have RBS).".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
            .
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..56
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     CDS             83..499
                     /codon_start=1
                     /product="gam protein"
                     /label="gam protein"
                     /note="recombination;138"
                     /db_xref="GI:15554347"
                     /protein_id="AAL02046.1"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             505..1290
                     /codon_start=1
                     /product="bet protein"
                     /label="bet protein"
                     /note="recombination;261"
                     /db_xref="GI:15554348"
                     /protein_id="AAL02047.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             1287..1967
                     /codon_start=1
                     /product="exonuclease"
                     /label="exonuclease"
                     /note="exo"
                     /db_xref="GI:15554349"
                     /protein_id="AAL02048.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     misc_feature    1992..2012
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    2013..2036
                     /label="Downstream Module"
     terminator      complement(2042..2144)
                     /label="T0 terminator"
     primer_bind     complement(2100..2116)
                     /label="PS2"
     primer_bind     2100..2116
                     /label="PS3"
     CDS             2305..3165
                     /label="AmpR"
     primer_bind     3236..3253
                     /label="PS5"
     misc_feature    complement(3238..3442)
                     /label="OriT"
     primer_bind     complement(3443..3460)
                     /label="PS4"
     rep_origin      complement(3504..4371)
                     /direction=LEFT
                     /label="pUC Ori"
     primer_bind     complement(4066..4076)
                     /label="PS6"
     terminator      complement(4417..4521)
                     /label="T1 terminator"
     primer_bind     complement(4420..4437)
                     /label="PS6"
     primer_bind     4420..4437
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc taatggatat taatactgaa actgagatca agcaaaagca
      121 ttcactaacc ccctttcctg ttttcctaat cagcccggca tttcgcgggc gatattttca
      181 cagctatttc aggagttcag ccatgaacgc ttattacatt caggatcgtc ttgaggctca
      241 gagctgggcg cgtcactacc agcagctcgc ccgtgaagag aaagaggcag aactggcaga
      301 cgacatggaa aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg atcatttgca
      361 acgccacggg gccagcaaaa aatccattac ccgtgcgttt gatgacgatg ttgagtttca
      421 ggagcgcatg gcagaacaca tccggtacat ggttgaaacc attgctcacc accaggttga
      481 tattgattca gaggtataaa acgaatgagt actgcactcg caacgctggc tgggaagctg
      541 gctgaacgtg tcggcatgga ttctgtcgac ccacaggaac tgatcaccac tcttcgccag
      601 acggcattta aaggtgatgc cagtgatgcg cagttcattg cattactgat cgttgccaac
      661 cagtacggcc ttaatccgtg gacgaaagaa atttacgcct ttcctgataa gcagaatggc
      721 atcgttccgg tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa ccagcagttt
      781 gatggcatgg actttgagca ggacaatgaa tcctgtacat gccggattta ccgcaaggac
      841 cgtaatcatc cgatctgcgt taccgaatgg atggatgaat gccgccgcga accattcaaa
      901 actcgcgaag gcagagaaat cacggggccg tggcagtcgc atcccaaacg gatgttacgt
      961 cataaagcca tgattcagtg tgcccgtctg gccttcggat ttgctggtat ctatgacaag
     1021 gatgaagccg agcgcattgt cgaaaatact gcatacactg ctgaacgtca gccggaacgc
     1081 gacatcactc cggttaacga tgaaaccatg caggagatta acactctgct gatcgccctg
     1141 gataaaacat gggatgacga cttattgccg ctctgttccc agatatttcg ccgcgacatt
     1201 cgtgcatcgt cagaactgac acaggccgaa gcagtaaaag ctcttggatt cctgaaacag
     1261 aaagccgcag agcagaaggt ggcagcatga caccggacat tatcctccag cgtaccggga
     1321 tcgatgtgag agctgtcgaa cagggggatg atgcgtggca caaattacgg ctcggcgtca
     1381 tcaccgcttc agaagttcac aacgtgatag caaaaccccg ctccggaaag aagtggcctg
     1441 acatgaaaat gtcctacttc cacaccctgc ttgctgaggt ttgcaccggt gtggctccgg
     1501 aagttaacgc taaagcactg gcctggggaa aacagtacga gaacgacgcc agaaccctgt
     1561 ttgagttcac ttccggcgtg aatgttactg aatccccgat catctatcgc gacgaaagta
     1621 tgcgtaccgc ctgctctccc gatggtttat gcagtgacgg caacggcctt gaactgaaat
     1681 gcccgtttac ctcccgggat ttcatgaagt tccggctcgg tggtttcgag gccataaagt
     1741 cagcttacat ggcccaggtg cagtacagca tgtgggtgac gcgaaaaaat gcctggtact
     1801 ttgccaacta tgacccgcgt atgaagcgtg aaggcctgca ttatgtcgtg attgagcggg
     1861 atgaaaagta catggcgagt tttgacgaga tcgtgccgga gttcatcgaa aaaatggacg
     1921 aggcactggc tgaaattggt tttgtatttg gggagcaatg gcgataagct ttgagacctt
     1981 tcatcgcgac ctactagtag cggccgctgc agggagttgt cttcgaagac ttcgctctag
     2041 tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag
     2101 aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc ccaataatta
     2161 cgatttaaat tagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta
     2221 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2281 caataatatt gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc gctgattccg
     2341 ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg aaaccctggt gaaagtgaaa
     2401 gatgcggaag atcaactggg tgcgcgcgtg ggctatattg aactggatct gaacagcggc
     2461 aaaattctgg aatcttttcg tccggaagaa cgttttccga tgatgagcac ctttaaagtg
     2521 ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc aggaacaact gggccgtcgt
     2581 attcattata gccagaacga tctggtggaa tatagcccgg tgaccgaaaa acatctgacc
     2641 gatggcatga ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga taacaccgcg
     2701 gcgaacctgc tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt tctgcataac
     2761 atgggcgatc atgtgacccg tctggatcgt tgggaaccgg aactgaacga agcgattccg
     2821 aacgatgaac gtgataccac catgccggca gcaatggcga ccaccctgcg taaactgctg
     2881 acgggtgagc tgctgaccct ggcaagccgc cagcaactga ttgattggat ggaagcggat
     2941 aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg gctggtttat tgcggataaa
     3001 agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg cgctgggccc ggatggtaaa
     3061 ccgagccgta ttgtggtgat ttataccacc ggcagccagg cgacgatgga tgaacgtaac
     3121 cgtcagattg cggaaattgg cgcgagcctg attaaacatt ggtaaaccga tacaattaaa
     3181 ggctcctttt ggagcctttt tttttggacg acccttgtcc ttttccgctg cataaccctg
     3241 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     3301 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     3361 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     3421 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg gccccgtaga
     3481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3601 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tttgagaaag
     3901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4141 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4201 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4261 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     4321 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     4381 gagttagctc actcattagg caggcgcgcc cagctgtcta gggcggcgga tttgtcctac
     4441 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4501 cctttcgttt tatttgatgc ct
//
