LOCUS       Exported                2660 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part N- N-terminus;
            polyhistidine tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     CDS             83..106
                     /codon_start=1
                     /label="Histag"
                     /translation="MHHHHHHG"
     misc_feature    130..150
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    151..174
                     /label="Downstream Module"
     terminator      complement(180..282)
                     /label="T0 terminator"
     primer_bind     complement(238..254)
                     /label="PS2"
     primer_bind     238..254
                     /label="PS3"
     CDS             443..1303
                     /label="AmpR"
     primer_bind     1374..1391
                     /label="PS5"
     misc_feature    complement(1376..1580)
                     /label="OriT"
     primer_bind     complement(1581..1598)
                     /label="PS4"
     rep_origin      complement(1642..2509)
                     /direction=LEFT
                     /label="pUC Ori"
     primer_bind     complement(2204..2214)
                     /label="PS6"
     terminator      complement(2555..2659)
                     /label="T1 terminator"
     primer_bind     complement(2558..2575)
                     /label="PS6"
     primer_bind     2558..2575
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc taatgcatca ccatcaccat cacggagcct gagacctttc
      121 atcgcgacct actagtagcg gccgctgcag ggagttgtct tcgaagactt cgctctagtc
      181 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      241 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
      301 atttaaatta gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt
      361 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      421 ataatattga aaaaggaaga gtatgagcat tcagcatttt cgtgtggcgc tgattccgtt
      481 ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa accctggtga aagtgaaaga
      541 tgcggaagat caactgggtg cgcgcgtggg ctatattgaa ctggatctga acagcggcaa
      601 aattctggaa tcttttcgtc cggaagaacg ttttccgatg atgagcacct ttaaagtgct
      661 gctgtgcggt gcggttctga gccgtgtgga tgcgggccag gaacaactgg gccgtcgtat
      721 tcattatagc cagaacgatc tggtggaata tagcccggtg accgaaaaac atctgaccga
      781 tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc atgagcgata acaccgcggc
      841 gaacctgctg ctgacgacca ttggcggtcc gaaagaactg accgcgtttc tgcataacat
      901 gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa
      961 cgatgaacgt gataccacca tgccggcagc aatggcgacc accctgcgta aactgctgac
     1021 gggtgagctg ctgaccctgg caagccgcca gcaactgatt gattggatgg aagcggataa
     1081 agtggcgggt ccgctgctgc gtagcgcgct gccggctggc tggtttattg cggataaaag
     1141 cggtgcgggc gaacgtggca gccgtggcat tattgcggcg ctgggcccgg atggtaaacc
     1201 gagccgtatt gtggtgattt ataccaccgg cagccaggcg acgatggatg aacgtaaccg
     1261 tcagattgcg gaaattggcg cgagcctgat taaacattgg taaaccgata caattaaagg
     1321 ctccttttgg agcctttttt tttggacgac ccttgtcctt ttccgctgca taaccctgct
     1381 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     1441 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     1501 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     1561 tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc cccgtagaaa
     1621 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1681 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1741 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1801 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1861 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1921 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1981 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagctt tgagaaagcg
     2041 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2101 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2161 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2221 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2281 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2341 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2401 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2461 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2521 gttagctcac tcattaggca ggcgcgccca gctgtctagg gcggcggatt tgtcctactc
     2581 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
     2641 tttcgtttta tttgatgcct
//
