LOCUS       Exported                2661 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part C- C-terminus;
            polyhistidine tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..38
                     /label="Upstream Module"
     misc_feature    39..60
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     CDS             86..103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label="Histag"
                     /translation="HHHHHH"
     misc_feature    131..151
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    152..175
                     /label="Downstream Module"
     terminator      complement(181..283)
                     /label="T0 terminator"
     primer_bind     complement(239..255)
                     /label="PS2"
     primer_bind     239..255
                     /label="PS3"
     CDS             444..1304
                     /label="AmpR"
     primer_bind     1375..1392
                     /label="PS5"
     misc_feature    complement(1377..1581)
                     /label="OriT"
     primer_bind     complement(1582..1599)
                     /label="PS4"
     rep_origin      complement(1643..2510)
                     /direction=LEFT
                     /label="pUC Ori"
     primer_bind     complement(2205..2215)
                     /label="PS6"
     terminator      complement(2556..2660)
                     /label="T1 terminator"
     primer_bind     complement(2559..2576)
                     /label="PS6"
     primer_bind     2559..2576
                     /label="PS1"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tttcgcatca ccatcaccat cactaagctt tgagaccttt
      121 catcgcgacc tactagtagc ggccgctgca gggagttgtc ttcgaagact tcgctctagt
      181 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
      241 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac
      301 gatttaaatt agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat
      361 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      421 aataatattg aaaaaggaag agtatgagca ttcagcattt tcgtgtggcg ctgattccgt
      481 tttttgcggc gttttgcctg ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag
      541 atgcggaaga tcaactgggt gcgcgcgtgg gctatattga actggatctg aacagcggca
      601 aaattctgga atcttttcgt ccggaagaac gttttccgat gatgagcacc tttaaagtgc
      661 tgctgtgcgg tgcggttctg agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta
      721 ttcattatag ccagaacgat ctggtggaat atagcccggt gaccgaaaaa catctgaccg
      781 atggcatgac cgtgcgtgaa ctgtgcagcg cggcgattac catgagcgat aacaccgcgg
      841 cgaacctgct gctgacgacc attggcggtc cgaaagaact gaccgcgttt ctgcataaca
      901 tgggcgatca tgtgacccgt ctggatcgtt gggaaccgga actgaacgaa gcgattccga
      961 acgatgaacg tgataccacc atgccggcag caatggcgac caccctgcgt aaactgctga
     1021 cgggtgagct gctgaccctg gcaagccgcc agcaactgat tgattggatg gaagcggata
     1081 aagtggcggg tccgctgctg cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa
     1141 gcggtgcggg cgaacgtggc agccgtggca ttattgcggc gctgggcccg gatggtaaac
     1201 cgagccgtat tgtggtgatt tataccaccg gcagccaggc gacgatggat gaacgtaacc
     1261 gtcagattgc ggaaattggc gcgagcctga ttaaacattg gtaaaccgat acaattaaag
     1321 gctccttttg gagccttttt ttttggacga cccttgtcct tttccgctgc ataaccctgc
     1381 ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga
     1441 ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg
     1501 ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac
     1561 ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa
     1621 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1681 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1741 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1801 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1861 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1921 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1981 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct ttgagaaagc
     2041 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2101 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2161 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2221 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2281 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2341 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2401 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2461 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2521 agttagctca ctcattaggc aggcgcgccc agctgtctag ggcggcggat ttgtcctact
     2581 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
     2641 ctttcgtttt atttgatgcc t
//
