LOCUS       Exported                4076 bp    ds-DNA  circular SYN 22-JUN-2018
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part T- Terminator;
            B0015. Terminator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..76
                     /label="Upstream Module"
     promoter        13..48
                     /label="J23100"
     misc_feature    77..100
                     /label="Duplicate ochre linker"
     misc_feature    101..122
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    123
                     /label="LVL-2 Main Module_minimal"
     terminator      160..231
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      247..274
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    305..308
                     /label="LVL-2 Main Module_minimal"
     misc_feature    309..329
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    330..1134
                     /label="Downstream Module"
     CDS             394..1104
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1140..1242)
                     /label="T0 terminator"
     primer_bind     complement(1198..1214)
                     /label="PS2"
     primer_bind     1198..1214
                     /label="PS3"
     CDS             1403..2263
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2334..2351
                     /label="PS5"
     misc_feature    complement(2336..2540)
                     /label="oriT"
     primer_bind     complement(2541..2558)
                     /label="PS4"
     rep_origin      2581..3200
                     /label="pBR322_origin"
     primer_bind     complement(3164..3174)
                     /label="PS6"
     misc_feature    complement(3186..3188)
                     /label="ORI"
     terminator      complement(3971..4055)
                     /label="T1 terminator"
     primer_bind     3974..3991
                     /label="PS1"
     primer_bind     complement(3974..3991)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctgctttcg accaggcatc aaataaaacg aaaggctcag
      181 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      241 cacactggct caccttcggg tgggcctttc tgcgtttata tgttcgcttg agacctttca
      301 tcgcgaccta ctagtagcgg ccgctgcagg gagtaacttc tgactgagtt gcatacttgt
      361 ttaactttaa gaaggagata tacaatggta gcccgtaaag gagaagaact tttcactgga
      421 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      481 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      541 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      601 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      661 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      721 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      781 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      841 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      901 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      961 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     1021 cccaacgaaa agagagatca catggtcctt cttgagtttg taacagctgc tgggattaca
     1081 catggcatgg atgaactata caaaggttcg taacttctga ctgagttgca cgctctagtc
     1141 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
     1201 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
     1261 atttaaatta gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt
     1321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1381 ataatattga aaaaggaaga gtatgagcat tcagcatttt cgtgtggcgc tgattccgtt
     1441 ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa accctggtga aagtgaaaga
     1501 tgcggaagat caactgggtg cgcgcgtggg ctatattgaa ctggatctga acagcggcaa
     1561 aattctggaa tcttttcgtc cggaagaacg ttttccgatg atgagcacct ttaaagtgct
     1621 gctgtgcggt gcggttctga gccgtgtgga tgcgggccag gaacaactgg gccgtcgtat
     1681 tcattatagc cagaacgatc tggtggaata tagcccggtg accgaaaaac atctgaccga
     1741 tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc atgagcgata acaccgcggc
     1801 gaacctgctg ctgacgacca ttggcggtcc gaaagaactg accgcgtttc tgcataacat
     1861 gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa
     1921 cgatgaacgt gataccacca tgccggcagc aatggcgacc accctgcgta aactgctgac
     1981 gggtgagctg ctgaccctgg caagccgcca gcaactgatt gattggatgg aagcggataa
     2041 agtggcgggt ccgctgctgc gtagcgcgct gccggctggc tggtttattg cggataaaag
     2101 cggtgcgggc gaacgtggca gccgtggcat tattgcggcg ctgggcccgg atggtaaacc
     2161 gagccgtatt gtggtgattt ataccaccgg cagccaggcg acgatggatg aacgtaaccg
     2221 tcagattgcg gaaattggcg cgagcctgat taaacattgg taaaccgata caattaaagg
     2281 ctccttttgg agcctttttt tttggacgac ccttgtcctt ttccgctgca taaccctgct
     2341 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     2401 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     2461 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     2521 tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc cccgtagaaa
     2581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2641 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2701 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2761 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2821 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2881 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2941 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3001 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3061 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3121 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3181 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3241 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3301 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3361 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3421 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     3481 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     3541 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga cagtctccgg
     3601 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
     3661 aagctcatca gcgtggtcgt gcagcgattc acagatgtct gcctgttcat ccgcgtccag
     3721 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
     3781 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
     3841 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     3901 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggggcg
     3961 cgcccagctg tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     4021 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcct
//
