LOCUS       Exported                4008 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part T- Terminator;
            L3S2P21; synthetic terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..76
                     /label="Upstream Module"
     promoter        13..48
                     /label="J23100"
     misc_feature    77..100
                     /label="Duplicate Ochre linker"
     misc_feature    101..122
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    123
                     /label="LVL-2 Main Module_minimal"
     misc_feature    152..212
                     /label="Terminator L3S2P21"
     misc_feature    237..240
                     /label="LVL-2 Main Module_minimal"
     misc_feature    241..261
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    262..1066
                     /label="Downstream Module"
     CDS             326..1036
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1072..1174)
                     /label="T0 terminator"
     primer_bind     complement(1130..1146)
                     /label="PS2"
     primer_bind     1130..1146
                     /label="PS3"
     CDS             1335..2195
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2266..2283
                     /label="PS5"
     misc_feature    complement(2268..2472)
                     /label="oriT"
     primer_bind     complement(2473..2490)
                     /label="PS4"
     rep_origin      2513..3132
                     /label="pBR322_origin"
     primer_bind     complement(3096..3106)
                     /label="PS6"
     misc_feature    complement(3118..3120)
                     /label="ORI"
     terminator      complement(3903..3987)
                     /label="T1 terminator"
     primer_bind     3906..3923
                     /label="PS1"
     primer_bind     complement(3906..3923)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctgctttcg actcggtacc aaattccaga aaagaggcct
      181 cccgaaaggg gggccttttt tcgttttggt cctgttcgct tgagaccttt catcgcgacc
      241 tactagtagc ggccgctgca gggagtaact tctgactgag ttgcatactt gtttaacttt
      301 aagaaggaga tatacaatgg tagcccgtaa aggagaagaa cttttcactg gagttgtccc
      361 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      421 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      481 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      541 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      601 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      661 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      721 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      781 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      841 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      901 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      961 aaagagagat cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1021 ggatgaacta tacaaaggtt cgtaacttct gactgagttg cacgctctag tcttggactc
     1081 ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
     1141 tgccgccggg cgttttttat tggtgagaat ccaggggtcc ccaataatta cgatttaaat
     1201 tagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta tttttctaaa
     1261 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1321 gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc gctgattccg ttttttgcgg
     1381 cgttttgcct gccggtgttt gcgcatccgg aaaccctggt gaaagtgaaa gatgcggaag
     1441 atcaactggg tgcgcgcgtg ggctatattg aactggatct gaacagcggc aaaattctgg
     1501 aatcttttcg tccggaagaa cgttttccga tgatgagcac ctttaaagtg ctgctgtgcg
     1561 gtgcggttct gagccgtgtg gatgcgggcc aggaacaact gggccgtcgt attcattata
     1621 gccagaacga tctggtggaa tatagcccgg tgaccgaaaa acatctgacc gatggcatga
     1681 ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga taacaccgcg gcgaacctgc
     1741 tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt tctgcataac atgggcgatc
     1801 atgtgacccg tctggatcgt tgggaaccgg aactgaacga agcgattccg aacgatgaac
     1861 gtgataccac catgccggca gcaatggcga ccaccctgcg taaactgctg acgggtgagc
     1921 tgctgaccct ggcaagccgc cagcaactga ttgattggat ggaagcggat aaagtggcgg
     1981 gtccgctgct gcgtagcgcg ctgccggctg gctggtttat tgcggataaa agcggtgcgg
     2041 gcgaacgtgg cagccgtggc attattgcgg cgctgggccc ggatggtaaa ccgagccgta
     2101 ttgtggtgat ttataccacc ggcagccagg cgacgatgga tgaacgtaac cgtcagattg
     2161 cggaaattgg cgcgagcctg attaaacatt ggtaaaccga tacaattaaa ggctcctttt
     2221 ggagcctttt tttttggacg acccttgtcc ttttccgctg cataaccctg cttcggggtc
     2281 attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa
     2341 agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa
     2401 gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg
     2461 ctcaacggga atcctgctct gcgaggctgg ccgtaggccg gccccgtaga aaagatcaaa
     2521 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2581 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2641 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     2701 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2761 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2821 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2881 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2941 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3001 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3061 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3121 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3181 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3241 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3301 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     3361 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     3421 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     3481 gggcttgtct gctcccggca tccgcttaca gacaagctgt gacagtctcc gggagctgca
     3541 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
     3601 cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
     3661 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     3721 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
     3781 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     3841 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcggggg cgcgcccagc
     3901 tgtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3961 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcct
//
