LOCUS       Exported                3999 bp    ds-DNA  circular SYN 22-JUN-2018
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part T- Terminator;
            L3S1P32; synthetic terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..76
                     /label="Upstream Module"
     promoter        13..48
                     /label="J23100"
     misc_feature    101..122
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    123
                     /label="LVL-2 Main Module_minimal"
     misc_feature    228..231
                     /label="LVL-2 Main Module_minimal"
     misc_feature    232..252
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    253..1057
                     /label="Downstream Module"
     CDS             317..1027
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1063..1165)
                     /label="T0 terminator"
     primer_bind     complement(1121..1137)
                     /label="PS2"
     primer_bind     1121..1137
                     /label="PS3"
     CDS             1326..2186
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2257..2274
                     /label="PS5"
     misc_feature    complement(2259..2463)
                     /label="oriT"
     primer_bind     complement(2464..2481)
                     /label="PS4"
     rep_origin      2504..3123
                     /label="pBR322_origin"
     primer_bind     complement(3087..3097)
                     /label="PS6"
     misc_feature    complement(3109..3111)
                     /label="ORI"
     terminator      complement(3894..3978)
                     /label="T1 terminator"
     primer_bind     3897..3914
                     /label="PS1"
     primer_bind     complement(3897..3914)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctgctttcg agacgaacaa taaggcctcc caaatcgggg
      181 ggccttttta tttttcaaca aaatgttcgc ttgagacctt tcatcgcgac ctactagtag
      241 cggccgctgc agggagtaac ttctgactga gttgcatact tgtttaactt taagaaggag
      301 atatacaatg gtagcccgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      361 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      421 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      481 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
      541 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      601 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      661 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      721 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      781 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      841 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      901 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      961 tcacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1021 atacaaaggt tcgtaacttc tgactgagtt gcacgctcta gtcttggact cctgttgata
     1081 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     1141 gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa ttagtagccc
     1201 gcctaatgag cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa
     1261 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1321 agagtatgag cattcagcat tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc
     1381 tgccggtgtt tgcgcatccg gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg
     1441 gtgcgcgcgt gggctatatt gaactggatc tgaacagcgg caaaattctg gaatcttttc
     1501 gtccggaaga acgttttccg atgatgagca cctttaaagt gctgctgtgc ggtgcggttc
     1561 tgagccgtgt ggatgcgggc caggaacaac tgggccgtcg tattcattat agccagaacg
     1621 atctggtgga atatagcccg gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg
     1681 aactgtgcag cgcggcgatt accatgagcg ataacaccgc ggcgaacctg ctgctgacga
     1741 ccattggcgg tccgaaagaa ctgaccgcgt ttctgcataa catgggcgat catgtgaccc
     1801 gtctggatcg ttgggaaccg gaactgaacg aagcgattcc gaacgatgaa cgtgatacca
     1861 ccatgccggc agcaatggcg accaccctgc gtaaactgct gacgggtgag ctgctgaccc
     1921 tggcaagccg ccagcaactg attgattgga tggaagcgga taaagtggcg ggtccgctgc
     1981 tgcgtagcgc gctgccggct ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg
     2041 gcagccgtgg cattattgcg gcgctgggcc cggatggtaa accgagccgt attgtggtga
     2101 tttataccac cggcagccag gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg
     2161 gcgcgagcct gattaaacat tggtaaaccg atacaattaa aggctccttt tggagccttt
     2221 ttttttggac gacccttgtc cttttccgct gcataaccct gcttcggggt cattatagcg
     2281 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     2341 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
     2401 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     2461 aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag aaaagatcaa aggatcttct
     2521 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2581 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2641 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2701 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2761 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2821 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2881 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2941 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3001 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3061 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3121 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3181 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3241 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     3301 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     3361 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     3421 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3481 tgctcccggc atccgcttac agacaagctg tgacagtctc cgggagctgc atgtgtcaga
     3541 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     3601 cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     3661 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     3721 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     3781 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     3841 gttgtgaggg taaacaactg gcggtatgga tgcggcgggg gcgcgcccag ctgtctaggg
     3901 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3961 ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
