LOCUS       Exported                3997 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part T- Terminator;
            L3S1P11; synthetic terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..76
                     /label="Upstream Module"
     promoter        13..48
                     /label="J23100"
     misc_feature    77..100
                     /label="Duplicate ochre linker"
     misc_feature    101..122
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    123
                     /label="LVL-2 Main Module_minimal"
     misc_feature    152..205
                     /label="Terminator L3S1P51"
     misc_feature    226..229
                     /label="LVL-2 Main Module_minimal"
     misc_feature    230..250
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    251..1055
                     /label="Downstream Module"
     CDS             315..1025
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1061..1163)
                     /label="T0 terminator"
     primer_bind     complement(1119..1135)
                     /label="PS2"
     primer_bind     1119..1135
                     /label="PS3"
     CDS             1324..2184
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2255..2272
                     /label="PS5"
     misc_feature    complement(2257..2461)
                     /label="oriT"
     primer_bind     complement(2462..2479)
                     /label="PS4"
     rep_origin      2502..3121
                     /label="pBR322_origin"
     primer_bind     complement(3085..3095)
                     /label="PS6"
     misc_feature    complement(3107..3109)
                     /label="ORI"
     terminator      complement(3892..3976)
                     /label="T1 terminator"
     primer_bind     3895..3912
                     /label="PS1"
     primer_bind     complement(3895..3912)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctgctttcg agacgaacaa taaggcctcc cttcgggggg
      181 gcctttttta ttgatacaaa atgttcgctt gagacctttc atcgcgacct actagtagcg
      241 gccgctgcag ggagtaactt ctgactgagt tgcatacttg tttaacttta agaaggagat
      301 atacaatggt agcccgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      361 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      421 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      481 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      541 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      601 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      661 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      721 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      781 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      841 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      901 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagatc
      961 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1021 acaaaggttc gtaacttctg actgagttgc acgctctagt cttggactcc tgttgataga
     1081 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc
     1141 gttttttatt ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc
     1201 ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat
     1261 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1321 agtatgagca ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg
     1381 ccggtgtttg cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt
     1441 gcgcgcgtgg gctatattga actggatctg aacagcggca aaattctgga atcttttcgt
     1501 ccggaagaac gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg
     1561 agccgtgtgg atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat
     1621 ctggtggaat atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa
     1681 ctgtgcagcg cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc
     1741 attggcggtc cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt
     1801 ctggatcgtt gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc
     1861 atgccggcag caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg
     1921 gcaagccgcc agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg
     1981 cgtagcgcgc tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc
     2041 agccgtggca ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt
     2101 tataccaccg gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc
     2161 gcgagcctga ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt
     2221 ttttggacga cccttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
     2281 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
     2341 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
     2401 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
     2461 tcctgctctg cgaggctggc cgtaggccgg ccccgtagaa aagatcaaag gatcttcttg
     2521 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2581 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2641 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2701 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2761 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2821 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2881 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2941 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3001 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3061 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3121 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3181 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3241 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     3301 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     3361 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     3421 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3481 ctcccggcat ccgcttacag acaagctgtg acagtctccg ggagctgcat gtgtcagagg
     3541 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     3601 tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     3661 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     3721 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     3781 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     3841 tgtgagggta aacaactggc ggtatggatg cggcgggggc gcgcccagct gtctagggcg
     3901 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3961 agtctttcga ctgagccttt cgttttattt gatgcct
//
