LOCUS       Exported                4079 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part CT- C-terminus +
            Terminator; B0015. C-termini STOP + Terminator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..76
                     /label="Upstream Module"
     promoter        13..48
                     /label="J23100"
     misc_feature    77..100
                     /label="Duplicate Ochre linker"
     misc_feature    101..122
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    123
                     /label="LVL-2 Main Module_minimal"
     terminator      163..234
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      250..277
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    308..311
                     /label="LVL-2 Main Module_minimal"
     misc_feature    312..332
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    333..1137
                     /label="Downstream Module"
     CDS             397..1107
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1143..1245)
                     /label="T0 terminator"
     primer_bind     complement(1201..1217)
                     /label="PS2"
     primer_bind     1201..1217
                     /label="PS3"
     CDS             1406..2266
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2337..2354
                     /label="PS5"
     misc_feature    complement(2339..2543)
                     /label="oriT"
     primer_bind     complement(2544..2561)
                     /label="PS4"
     rep_origin      2584..3203
                     /label="pBR322_origin"
     primer_bind     complement(3167..3177)
                     /label="PS6"
     misc_feature    complement(3189..3191)
                     /label="ORI"
     terminator      complement(3974..4058)
                     /label="T1 terminator"
     primer_bind     3977..3994
                     /label="PS1"
     primer_bind     complement(3977..3994)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctttcgtga tcgaccaggc atcaaataaa acgaaaggct
      181 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      241 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatgttcgc ttgagacctt
      301 tcatcgcgac ctactagtag cggccgctgc agggagtaac ttctgactga gttgcatact
      361 tgtttaactt taagaaggag atatacaatg gtagcccgta aaggagaaga acttttcact
      421 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      481 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      541 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
      601 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
      661 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      721 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      781 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
      841 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
      901 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      961 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
     1021 gatcccaacg aaaagagaga tcacatggtc cttcttgagt ttgtaacagc tgctgggatt
     1081 acacatggca tggatgaact atacaaaggt tcgtaacttc tgactgagtt gcacgctcta
     1141 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1201 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
     1261 acgatttaaa ttagtagccc gcctaatgag cgggcttttt tttaattccc ctatttgttt
     1321 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1381 tcaataatat tgaaaaagga agagtatgag cattcagcat tttcgtgtgg cgctgattcc
     1441 gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg gaaaccctgg tgaaagtgaa
     1501 agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt gaactggatc tgaacagcgg
     1561 caaaattctg gaatcttttc gtccggaaga acgttttccg atgatgagca cctttaaagt
     1621 gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc caggaacaac tgggccgtcg
     1681 tattcattat agccagaacg atctggtgga atatagcccg gtgaccgaaa aacatctgac
     1741 cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt accatgagcg ataacaccgc
     1801 ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa ctgaccgcgt ttctgcataa
     1861 catgggcgat catgtgaccc gtctggatcg ttgggaaccg gaactgaacg aagcgattcc
     1921 gaacgatgaa cgtgatacca ccatgccggc agcaatggcg accaccctgc gtaaactgct
     1981 gacgggtgag ctgctgaccc tggcaagccg ccagcaactg attgattgga tggaagcgga
     2041 taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct ggctggttta ttgcggataa
     2101 aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg gcgctgggcc cggatggtaa
     2161 accgagccgt attgtggtga tttataccac cggcagccag gcgacgatgg atgaacgtaa
     2221 ccgtcagatt gcggaaattg gcgcgagcct gattaaacat tggtaaaccg atacaattaa
     2281 aggctccttt tggagccttt ttttttggac gacccttgtc cttttccgct gcataaccct
     2341 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
     2401 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
     2461 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
     2521 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag
     2581 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2641 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2701 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     2761 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2821 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2881 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2941 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3001 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3061 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3121 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3181 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3301 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3361 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3421 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     3481 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     3541 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgacagtctc
     3601 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     3661 gtaaagctca tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc
     3721 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     3781 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     3841 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     3901 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcgggg
     3961 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
     4021 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
