LOCUS       Exported                4011 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part CT- C-terminus +
            Terminator; L3S2P21. C-termini STOP + terminator (synthetic).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..76
                     /label="Upstream Module"
     promoter        13..48
                     /label="J23100"
     misc_feature    77..100
                     /label="duplicate linker in T acceptor"
     misc_feature    101..122
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    123
                     /label="LVL-2 Main Module_minimal"
     terminator      155..215
                     /label="Terminator L3S2P21"
     misc_feature    240..243
                     /label="LVL-2 Main Module_minimal"
     misc_feature    244..264
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    265..1069
                     /label="Downstream Module"
     CDS             329..1039
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1075..1177)
                     /label="T0 terminator"
     primer_bind     complement(1133..1149)
                     /label="PS2"
     primer_bind     1133..1149
                     /label="PS3"
     CDS             1338..2198
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2269..2286
                     /label="PS5"
     misc_feature    complement(2271..2475)
                     /label="oriT"
     primer_bind     complement(2476..2493)
                     /label="PS4"
     rep_origin      2516..3135
                     /label="pBR322_origin"
     primer_bind     complement(3099..3109)
                     /label="PS6"
     misc_feature    complement(3121..3123)
                     /label="ORI"
     terminator      complement(3906..3990)
                     /label="T1 terminator"
     primer_bind     3909..3926
                     /label="PS1"
     primer_bind     complement(3909..3926)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctttcgtga tcgactcggt accaaattcc agaaaagagg
      181 cctcccgaaa ggggggcctt ttttcgtttt ggtcctgttc gcttgagacc tttcatcgcg
      241 acctactagt agcggccgct gcagggagta acttctgact gagttgcata cttgtttaac
      301 tttaagaagg agatatacaa tggtagcccg taaaggagaa gaacttttca ctggagttgt
      361 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      421 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      481 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc
      541 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
      601 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
      661 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
      721 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
      781 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
      841 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      901 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      961 cgaaaagaga gatcacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     1021 catggatgaa ctatacaaag gttcgtaact tctgactgag ttgcacgctc tagtcttgga
     1081 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     1141 ggttgccgcc gggcgttttt tattggtgag aatccagggg tccccaataa ttacgattta
     1201 aattagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct
     1261 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1321 attgaaaaag gaagagtatg agcattcagc attttcgtgt ggcgctgatt ccgttttttg
     1381 cggcgttttg cctgccggtg tttgcgcatc cggaaaccct ggtgaaagtg aaagatgcgg
     1441 aagatcaact gggtgcgcgc gtgggctata ttgaactgga tctgaacagc ggcaaaattc
     1501 tggaatcttt tcgtccggaa gaacgttttc cgatgatgag cacctttaaa gtgctgctgt
     1561 gcggtgcggt tctgagccgt gtggatgcgg gccaggaaca actgggccgt cgtattcatt
     1621 atagccagaa cgatctggtg gaatatagcc cggtgaccga aaaacatctg accgatggca
     1681 tgaccgtgcg tgaactgtgc agcgcggcga ttaccatgag cgataacacc gcggcgaacc
     1741 tgctgctgac gaccattggc ggtccgaaag aactgaccgc gtttctgcat aacatgggcg
     1801 atcatgtgac ccgtctggat cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg
     1861 aacgtgatac caccatgccg gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg
     1921 agctgctgac cctggcaagc cgccagcaac tgattgattg gatggaagcg gataaagtgg
     1981 cgggtccgct gctgcgtagc gcgctgccgg ctggctggtt tattgcggat aaaagcggtg
     2041 cgggcgaacg tggcagccgt ggcattattg cggcgctggg cccggatggt aaaccgagcc
     2101 gtattgtggt gatttatacc accggcagcc aggcgacgat ggatgaacgt aaccgtcaga
     2161 ttgcggaaat tggcgcgagc ctgattaaac attggtaaac cgatacaatt aaaggctcct
     2221 tttggagcct ttttttttgg acgacccttg tccttttccg ctgcataacc ctgcttcggg
     2281 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     2341 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     2401 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     2461 gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggccccgt agaaaagatc
     2521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2641 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3181 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3241 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3301 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     3361 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     3421 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     3481 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgacagtc tccgggagct
     3541 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     3601 catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     3661 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     3721 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     3781 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     3841 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gggcgcgccc
     3901 agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3961 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc t
//
