LOCUS       Exported                4002 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part CT- C-terminus +
            Terminator; L3S1P51. C-termini STOP + terminator (synthetic).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..76
                     /label="Upstream Module"
     promoter        13..48
                     /label="J23100"
     misc_feature    77..100
                     /label="Duplicate linker in T acceptor"
     misc_feature    101..122
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    123
                     /label="LVL-2 Main Module_minimal"
     terminator      155..206
                     /label="Terminator L3S1P51"
     misc_feature    231..234
                     /label="LVL-2 Main Module_minimal"
     misc_feature    235..255
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    256..1060
                     /label="Downstream Module"
     CDS             320..1030
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1066..1168)
                     /label="T0 terminator"
     primer_bind     complement(1124..1140)
                     /label="PS2"
     primer_bind     1124..1140
                     /label="PS3"
     CDS             1329..2189
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2260..2277
                     /label="PS5"
     misc_feature    complement(2262..2466)
                     /label="oriT"
     primer_bind     complement(2467..2484)
                     /label="PS4"
     rep_origin      2507..3126
                     /label="pBR322_origin"
     primer_bind     complement(3090..3100)
                     /label="PS6"
     misc_feature    complement(3112..3114)
                     /label="ORI"
     terminator      complement(3897..3981)
                     /label="T1 terminator"
     primer_bind     3900..3917
                     /label="PS1"
     primer_bind     complement(3900..3917)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctttcgtga tcgaaaaaaa aaaaaaggcc tcccaaatcg
      181 gggggccttt tttattgata acaaaatgtt cgcttgagac ctttcatcgc gacctactag
      241 tagcggccgc tgcagggagt aacttctgac tgagttgcat acttgtttaa ctttaagaag
      301 gagatataca atggtagccc gtaaaggaga agaacttttc actggagttg tcccaattct
      361 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      421 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      481 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
      541 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      601 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      661 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
      721 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
      781 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
      841 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
      901 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
      961 agatcacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1021 actatacaaa ggttcgtaac ttctgactga gttgcacgct ctagtcttgg actcctgttg
     1081 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
     1141 cgggcgtttt ttattggtga gaatccaggg gtccccaata attacgattt aaattagtag
     1201 cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt
     1261 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1321 ggaagagtat gagcattcag cattttcgtg tggcgctgat tccgtttttt gcggcgtttt
     1381 gcctgccggt gtttgcgcat ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac
     1441 tgggtgcgcg cgtgggctat attgaactgg atctgaacag cggcaaaatt ctggaatctt
     1501 ttcgtccgga agaacgtttt ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg
     1561 ttctgagccg tgtggatgcg ggccaggaac aactgggccg tcgtattcat tatagccaga
     1621 acgatctggt ggaatatagc ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc
     1681 gtgaactgtg cagcgcggcg attaccatga gcgataacac cgcggcgaac ctgctgctga
     1741 cgaccattgg cggtccgaaa gaactgaccg cgtttctgca taacatgggc gatcatgtga
     1801 cccgtctgga tcgttgggaa ccggaactga acgaagcgat tccgaacgat gaacgtgata
     1861 ccaccatgcc ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt gagctgctga
     1921 ccctggcaag ccgccagcaa ctgattgatt ggatggaagc ggataaagtg gcgggtccgc
     1981 tgctgcgtag cgcgctgccg gctggctggt ttattgcgga taaaagcggt gcgggcgaac
     2041 gtggcagccg tggcattatt gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg
     2101 tgatttatac caccggcagc caggcgacga tggatgaacg taaccgtcag attgcggaaa
     2161 ttggcgcgag cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc
     2221 tttttttttg gacgaccctt gtccttttcc gctgcataac cctgcttcgg ggtcattata
     2281 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
     2341 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc
     2401 ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac
     2461 gggaatcctg ctctgcgagg ctggccgtag gccggccccg tagaaaagat caaaggatct
     2521 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2581 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2641 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2701 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2761 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2821 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2881 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2941 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3001 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3061 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3121 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3181 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3241 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     3301 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     3361 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     3421 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3481 gtctgctccc ggcatccgct tacagacaag ctgtgacagt ctccgggagc tgcatgtgtc
     3541 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     3601 ggtcgtgcag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     3661 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     3721 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     3781 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     3841 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggggcgcgcc cagctgtcta
     3901 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     3961 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ct
//
