LOCUS       Exported                3979 bp    ds-DNA  circular SYN 23-APR-2019
DEFINITION  JUMP - Joint Universal Modular Plasmid. Basic Part CT- C-terminus +
            Terminator; L1U1H08. C-termini STOP + terminator (synthetic).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Marcos Valenzuela-Ortega and Chris French
  TITLE     The JUMP toolkit:Joint Universal Modular Plasmids for synthetic
            biology
  JOURNAL   Publication in preparation
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="JUMP vector"
                     /mol_type="other DNA"
     misc_feature    9..76
                     /label="Upstream Module"
     promoter        13..48
                     /label="J23100"
     misc_feature    101..122
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     ATG"
     misc_feature    123
                     /label="LVL-2 Main Module_minimal"
     misc_feature    155..183
                     /label="Terminator L1U1H08"
     misc_feature    208..211
                     /label="LVL-2 Main Module_minimal"
     misc_feature    212..232
                     /label="BioBrick suffix"
                     /note="BioBrick universal suffix for all parts"
     misc_feature    233..1037
                     /label="Downstream Module"
     CDS             297..1007
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(1043..1145)
                     /label="T0 terminator"
     primer_bind     complement(1101..1117)
                     /label="PS2"
     primer_bind     1101..1117
                     /label="PS3"
     CDS             1306..2166
                     /label="Ap"
                     /note="Ampicillin Resistance"
     primer_bind     2237..2254
                     /label="PS5"
     misc_feature    complement(2239..2443)
                     /label="oriT"
     primer_bind     complement(2444..2461)
                     /label="PS4"
     rep_origin      2484..3103
                     /label="pBR322_origin"
     primer_bind     complement(3067..3077)
                     /label="PS6"
     misc_feature    complement(3089..3091)
                     /label="ORI"
     terminator      complement(3874..3958)
                     /label="T1 terminator"
     primer_bind     3877..3894
                     /label="PS1"
     primer_bind     complement(3877..3894)
                     /label="PS6"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctttcgtga tcgacccgca tgttcgcatg cgggtttttt
      181 ttttgttcgc ttgagacctt tcatcgcgac ctactagtag cggccgctgc agggagtaac
      241 ttctgactga gttgcatact tgtttaactt taagaaggag atatacaatg gtagcccgta
      301 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      361 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      421 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      481 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      541 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      601 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      661 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      721 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      781 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      841 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      901 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga tcacatggtc cttcttgagt
      961 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaaggt tcgtaacttc
     1021 tgactgagtt gcacgctcta gtcttggact cctgttgata gatccagtaa tgacctcaga
     1081 actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa
     1141 tccaggggtc cccaataatt acgatttaaa ttagtagccc gcctaatgag cgggcttttt
     1201 tttaattccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1261 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag cattcagcat
     1321 tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg
     1381 gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt
     1441 gaactggatc tgaacagcgg caaaattctg gaatcttttc gtccggaaga acgttttccg
     1501 atgatgagca cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc
     1561 caggaacaac tgggccgtcg tattcattat agccagaacg atctggtgga atatagcccg
     1621 gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt
     1681 accatgagcg ataacaccgc ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa
     1741 ctgaccgcgt ttctgcataa catgggcgat catgtgaccc gtctggatcg ttgggaaccg
     1801 gaactgaacg aagcgattcc gaacgatgaa cgtgatacca ccatgccggc agcaatggcg
     1861 accaccctgc gtaaactgct gacgggtgag ctgctgaccc tggcaagccg ccagcaactg
     1921 attgattgga tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct
     1981 ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg
     2041 gcgctgggcc cggatggtaa accgagccgt attgtggtga tttataccac cggcagccag
     2101 gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct gattaaacat
     2161 tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac gacccttgtc
     2221 cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     2281 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     2341 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     2401 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     2461 gccgtaggcc ggccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2521 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2581 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2641 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2701 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2761 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2821 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2881 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2941 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3001 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3061 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3121 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3181 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3241 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     3301 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     3361 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     3421 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3481 agacaagctg tgacagtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3541 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgcagcga ttcacagatg
     3601 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     3661 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     3721 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     3781 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     3841 gcggtatgga tgcggcgggg gcgcgcccag ctgtctaggg cggcggattt gtcctactca
     3901 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
     3961 ttcgttttat ttgatgcct
//
