LOCUS       Exported                7119 bp ds-DNA     circular SYN 30-APR-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-DIO-ChR2(H134R)-eYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 30, 2019 from SnapGene 4.3.6
            https://www.snapgene.com
COMMENT     LOCUS pAAV-EF1a-DIO-hC 7266 bp DNA circular 04-MAR-2016 SOURCE 
            ORGANISM KEYWORDS COMMENT This file is created by Vector NTI 
            http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|478987020| 
            VNTDBDATE|480873358| LSOWNER| VNTNAME|pAAV-EF1a-double floxed 
            ChR2-EYFP-WPRE| VNTAUTHORNAME|Deisseroth Lab| 
            VNTAUTHOREML|charur@stanford.edu| VNTOAUTHORNAME|Feng Zhang| 
            Vector_NTI_Display_Data_(Do_Not_Edit!) (SXF (CGexDoc 
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FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /label=L-ITR
                     /note="color: #ff8000"
     promoter        164..1263
                     /label=CAG
                     /note="CAG"
                     /note="color: #00ffe1; direction: RIGHT"
     primer_bind     164..199
                     /label=Primer 11
                     /note="color: black; sequence: 
                     TTATTAATAGTAATCAATTACGGGGTCATTAGTTCA"
     primer_bind     734..756
                     /label=CCGAAAGTTTCCTTTTATGGCGA
                     /note="color: black; sequence: CCGAAAGTTTCCTTTTATGGCGA"
     primer_bind     880..900
                     /label=CCGCGTTACTCCCACAGGTGA
                     /note="color: black; sequence: CCGCGTTACTCCCACAGGTGA"
     primer_bind     964..986
                     /label=Primer 14
                     /note="color: black; sequence: CTTTTCTGTGGCTGCGTGAAAGC"
     misc_feature    1303..1336
                     /label=lox2722
                     /note="color: #ff8000"
     misc_feature    1387..1420
                     /label=loxP
                     /note="color: #ff8000"
     exon            complement(1432..2148)
                     /label=EYFP (no start)
                     /note="color: #80ff80; direction: LEFT"
     primer_bind     complement(1471..1491)
                     /label=Primer 5
                     /note="color: black; sequence: CTGCTGGAGTTCGTGACCGCC"
     primer_bind     1549..1569
                     /label=Primer 2
                     /note="color: black; sequence: GTAGTGGTTGTCGGGCAGCAG"
     primer_bind     1993..2014
                     /label=Primer 1
                     /note="color: black; sequence: CTTGCCGGTGGTGCAGATGAAC"
     primer_bind     complement(2122..2143)
                     /label=Primer 6
                     /note="color: black; sequence: CAAGGGCGAGGAGCTGTTCACC"
     exon            complement(2164..3093)
                     /label=hChR2
                     /note="color: #80ff80; direction: LEFT"
     primer_bind     2345..2364
                     /label=Primer 7
                     /note="color: black; sequence: GTGTGACCTACGGTGGAGCC"
     variation       2692..2694
                     /label=H134R
                     /note="color: #ff0000"
     primer_bind     complement(2814..2838)
                     /label=Primer 3
                     /note="color: black; sequence: GTGTGCGCCATTGAAATGGTTAAGG"
     primer_bind     2922..2939
                     /label=Primer 8
                     /note="color: black; sequence: GCCACTGCAGGACATTTG"
     misc_feature    complement(3104..3137)
                     /label=lox2722
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    complement(3188..3221)
                     /label=loxP
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    3236..3844
                     /label=WPRE
                     /note="color: #ff8000"
     primer_bind     complement(3407..3429)
                     /label=Primer 4
                     /note="color: black; sequence: GCCACAACTCCTCATAAAGAGAC"
     primer_bind     3549..3566
                     /label=Primer 9
                     /note="color: black; sequence: GCCACGGCGGAACTCATC"
     polyA_signal    3864..4342
                     /label=hGH polyA
                     /note="color: #8000ff"
     primer_bind     complement(3964..3988)
                     /label=Primer 10
                     /note="color: black; sequence: GAAGGACACCTAGTCAGACAAAATG"
     misc_feature    4382..4522
                     /label=R-ITR
                     /note="color: #ff8000"
     rep_origin      4614..4920
                     /direction=RIGHT
                     /label=f1 Origin
                     /note="color: #c0c0c0"
     misc_feature    5439..6296
                     /label=AmpR
                     /note="color: #ff8000"
     rep_origin      6447..7114
                     /direction=RIGHT
                     /label=pUC ori
                     /note="color: #c0c0c0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaacac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      541 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      601 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
      661 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      721 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      781 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      841 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      901 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      961 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagcgccgg caggaaggaa
     1021 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1081 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1141 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1201 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1261 ttgcggccca acggtaccgg atcctctaga gtcgactccg gaataacttc gtataggata
     1321 ctttatacga agttatgcag aatggtagct ggattgtagc tgctattagc aatatgaaac
     1381 ctcttaataa cttcgtatag catacattat acgaagttat ggcgcgccct attacttgta
     1441 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     1501 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc
     1561 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     1621 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     1681 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     1741 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     1801 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     1861 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1921 gtcggggtag cgggcgaagc actgcaggcc gtagccgaag gtggtcacga gggtgggcca
     1981 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     2041 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     2101 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcggc
     2161 cgctggcacg gctccggcct cggcttcgtc ttcgacgaga gtctcgacct cgatctccgt
     2221 tccgccgatg ttcagtttgg tggttttgcg gatatctccg tgaatcaata tgtgctcgtg
     2281 gatcaggacg cgcaggtagt gtcccaacaa cccccaacaa tttttactca tcagatcaat
     2341 aatcgtgtga cctacggtgg agccatagac gctcaggacg ccaaaacctt cgggccccaa
     2401 aatgaagaga attgggaaca taccccagct cacgaaaaac agccatgcca tgccggtcac
     2461 gacctggcgg caccgaccct ttggcacagt atgataaccc tcgatatatg ctttggcggc
     2521 gtgaaaaaat gtgttcgcgc catagcacaa tccaagacaa aagaagatga ctttaacata
     2581 gccggttgcc atggcgctgg tagcccccca cacgatagtc ccgatgtctg agacaaggag
     2641 tcccatggtt ctcctgctgt agtcgttgct caggccggtg aggttgctca ggcggataag
     2701 gatgacagga caagtgagca gccactctgc atagcgcagc cactgcaccc ggtgtcctgt
     2761 ggcaaggtag agcatagagg gattcttaaa ctcaaaaaag aactcgagaa tcaccttaac
     2821 catttcaatg gcgcacacat agatctcctc ccagccgcat gtagatttcc aggtttggta
     2881 ggcatagaac atcagcagca aaatgctgaa tcctgctgca agccactgca ggacatttga
     2941 cgcggtctga gcgccgttcg tgccgcgaga ttcaatccat ccggcacagt aacattgatc
     3001 ctcagggacc aggacggacc cgttcaccac cacaggatta gtaacgaaca aaagttcgcg
     3061 tccgacggca gacaaagcgc cgccatagtc catggtggct agcataactt cgtataaagt
     3121 atcctatacg aagttatttg ccttaaccca gaaattatca ctgttattct ttagaatggt
     3181 gcaaagaata acttcgtata atgtatgcta tacgaagtta tgaattcgat atcaagctta
     3241 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3301 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3361 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3421 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3481 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3541 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3601 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3661 tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3721 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3781 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     3841 accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct
     3901 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     3961 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     4021 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     4081 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     4141 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     4201 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     4261 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     4321 cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg
     4381 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4441 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4501 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4561 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4621 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4681 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4741 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4801 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4861 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4921 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     4981 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5041 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5101 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5161 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5221 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5281 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5401 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5461 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5521 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5581 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5641 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5701 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5761 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5821 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5881 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5941 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6001 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6061 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6121 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6181 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6241 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6301 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6361 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6421 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6481 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6541 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6601 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6661 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6721 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6781 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6841 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6901 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6961 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7021 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7081 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
