LOCUS       Exported               14872 bp ds-DNA     circular SYN 23-APR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 30, 2019 from SnapGene Viewer 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14872
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2608..2848
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        2877..2952
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2989..3303
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2997..3015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3068..3086
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3104..3122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3140..3158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3175..3193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3211..3229
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      3329..3338
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3335..7438
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7439..7486
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7487..7510
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7520..7573
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7574..8290
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        8301..8512
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             8534..9130
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             9140..9196
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9197..9943
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    9964..10552
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10435..10446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10624..10857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10889..11113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11159..11587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        11601..11930
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11781..11916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        11978..12025
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12044..12418
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12548..12669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12718..12734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12742..12758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12766..12796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12811..12832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13120..13708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13879..14739)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14740..14844)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccgggg ccactaggga caggatgttt
     2881 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     2941 ccgagtcggt gcttttttga attcactttg gccgcgaatc gatatgtcga gtttactccc
     3001 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgatgtcg
     3061 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
     3121 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttatcccta
     3181 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     3241 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
     3301 gcctggagaa taccggttct agagcgctgc caccatggac aagaagtaca gcatcggcct
     3361 ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
     3421 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
     3481 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
     3541 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
     3601 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
     3661 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
     3721 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
     3781 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
     3841 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
     3901 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
     3961 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
     4021 gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac
     4081 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
     4141 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
     4201 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
     4261 caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca
     4321 ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga
     4381 gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca
     4441 ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact
     4501 gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag
     4561 catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt
     4621 ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc
     4681 ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag
     4741 cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca
     4801 gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc
     4861 caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata
     4921 cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt
     4981 ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt
     5041 caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc
     5101 ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa
     5161 tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag
     5221 agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa
     5281 gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg
     5341 catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc
     5401 caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca
     5461 gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg
     5521 cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa
     5581 agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac
     5641 cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa
     5701 agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga
     5761 gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga
     5821 catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga
     5881 cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa
     5941 cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc
     6001 caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag
     6061 cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa
     6121 gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct
     6181 gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga
     6241 tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct
     6301 gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt
     6361 gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat
     6421 cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga
     6481 gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac
     6541 cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat
     6601 gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc
     6661 tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa
     6721 gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt
     6781 ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat
     6841 ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga
     6901 agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg
     6961 ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc
     7021 ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc
     7081 cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat
     7141 catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa
     7201 agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat
     7261 catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac
     7321 caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca
     7381 ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacaa
     7441 gcgacctgcc gccacaaaga aggctggaca ggctaagaag aagaaagatt acaaagacga
     7501 tgacgataag ggatccggag agggcagagg aagtctgcta acatgcggtg acgtcgagga
     7561 gaatcctggc ccagtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     7621 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     7681 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     7741 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     7801 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     7861 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     7921 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     7981 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     8041 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     8101 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     8161 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     8221 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     8281 gtacaagtaa gctagcatcc gggcagagcg cacatcgccc acagtccccg agaagttggg
     8341 gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     8401 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     8461 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agactgcgat
     8521 cgcaatgtac agtatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     8581 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     8641 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     8701 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     8761 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     8821 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     8881 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     8941 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9001 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9061 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9121 gcccggtgcc ggttccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     9181 agagaatcct ggaccgatgt ctagactgga caagagcaaa gtcataaacg gagctctgga
     9241 attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg
     9301 agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct
     9361 gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg
     9421 gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga
     9481 cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa
     9541 tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc
     9601 cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa
     9661 agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga
     9721 gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg
     9781 tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt
     9841 tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc
     9901 tgacgctctt gacgattttg accttgacat gctccccggg taactcgagc gcgttaagtc
     9961 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
    10021 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
    10081 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
    10141 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
    10201 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    10261 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    10321 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    10381 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    10441 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    10501 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
    10561 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
    10621 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
    10681 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10741 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10801 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
    10861 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    10921 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    10981 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    11041 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    11101 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
    11161 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    11221 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    11281 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    11341 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    11401 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    11461 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    11521 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    11581 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
    11641 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
    11701 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
    11761 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    11821 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
    11881 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
    11941 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
    12001 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
    12061 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
    12121 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
    12181 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
    12241 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
    12301 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
    12361 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
    12421 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    12481 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    12541 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    12601 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    12661 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    12721 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    12781 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    12841 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    12901 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    12961 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    13021 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    13081 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    13141 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    13201 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    13261 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    13321 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    13381 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    13441 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    13501 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    13561 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    13621 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    13681 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    13741 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    13801 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    13861 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    13921 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    13981 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    14041 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    14101 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    14161 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    14221 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    14281 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    14341 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    14401 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    14461 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    14521 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    14581 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    14641 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    14701 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    14761 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    14821 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
