LOCUS       pENTR4_G3BP1            3945 bp    DNA     circular     28-FEB-2019
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
                     /note="recognized as attL1"
     misc_recomb     complement(2070..2168)
                     /label=attL2
                     /note="attL2 (c)"
                     /note="recognized as attL2"
     misc_signal     complement(2183..2202)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2210..2226
                     /note="M13 Reverse priming site"
     gene            2339..3148
                     /note="Kanamycin resistance gene"
     rep_origin      3269..3942
                     /note="pUC origin"
     source          669..2069
                     /dnas_title="pENTR4_G3BP1"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.1"
     CDS             669..2069
                     /dnas_title="G3BP1"
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /EC_number="3.6.4.13"
                     /EC_number="3.6.4.12"
                     /note="Ras-GTPase-activating protein SH3-domain-binding
                     protein; GAP binding protein; RasGAP-associated
                     endoribonuclease G3BP; G3BP-1; ATP-dependent DNA helicase
                     VIII; GAP SH3 domain-binding protein 1"
                     /codon_start=1
                     /product="ras GTPase-activating protein-binding protein 1"
                     /protein_id="NP_005745.1"
                     /db_xref="GI:5031703"
                     /db_xref="CCDS:CCDS4319.1"
                     /db_xref="GeneID:10146"
                     /db_xref="HGNC:30292"
                     /db_xref="HPRD:06569"
                     /db_xref="MIM:608431"
                     /translation="MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGG
                     LDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNN
                     QALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQ
                     QTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPV
                     LEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVK
                     VPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMV
                     RHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQ
                     KVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGM
                     VQKPGFGVGRGLAPRQ"
     PCR_product     complement(669..2069)
                     /pair="Pair 1"
                     /set="Set 1"
                     /current=1
                     /score="0.000"
     PCR_frame       669..2069
                     /note="Reading Frame"
                     /set="Set 1"
     insert          669..2069
                     /source="G3BP1 AttB Insert"
     PCR_frame       669..2069
                     /note="Reading Frame"
                     /set="Set 2"
     PCR_product     complement(669..2069)
                     /pair="Pair 1"
                     /set="Set 1"
                     /current=1
                     /score="0.000"
     PCR_product     complement(659..2079)
                     /pair="Pair 1"
                     /set="Set 2"
                     /current=1
                     /score="0.000"
     vector          join(2080..3945,1..658)
                     /source="pDONR%99221"
     clone           659..2079
                     /source="G3BP1 AttB Insert AttB Insert"
ORIGIN      
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTat ggtgatggag aagcctagtc ccctgctggt cgggcgggaa tttgtgagac
      721 agtattacac actgctgaac caggccccag acatgctgca tagattttat ggaaagaact
      781 cttcttatgt ccatggggga ttggattcaa atggaaagcc agcagatgca gtctacggac
      841 agaaagaaat ccacaggaaa gtgatgtcac aaaacttcac caactgccac accaagattc
      901 gccatgttga tgctcatgcc acgctaaatg atggtgtggt agtccaggtg atggggcttc
      961 tctctaacaa caaccaggct ttgaggagat tcatgcaaac gtttgtcctt gctcctgagg
     1021 ggtctgttgc aaataaattc tatgttcaca atgatatctt cagataccaa gatgaggtct
     1081 ttggtgggtt tgtcactgag cctcaggagg agtctgaaga agaagtagag gaacctgaag
     1141 aaagacagca aacacctgag gtggtacctg atgattctgg aactttctat gatcaggcag
     1201 ttgtcagtaa tgacatggaa gaacatttag aggagcctgt tgctgaacca gagcctgatc
     1261 ctgaaccaga accagaacaa gaacctgtat ctgaaatcca agaggaaaag cctgagccag
     1321 tattagaaga aactgcccct gaggatgctc agaagagttc ttctccagca cctgcagaca
     1381 tagctcagac agtacaggaa gacttgagga cattttcttg ggcatctgtg accagtaaga
     1441 atcttccacc cagtggagct gttccagtta ctgggatacc acctcatgtt gttaaagtac
     1501 cagcttcaca gccccgtcca gagtctaagc ctgaatctca gattccacca caaagacctc
     1561 agcgggatca aagagtgcga gaacaacgaa taaatattcc tccccaaagg ggacccagac
     1621 caatccgtga ggctggtgag caaggtgaca ttgaaccccg aagaatggtg agacaccctg
     1681 acagtcacca actcttcatt ggcaacctgc ctcatgaagt ggacaaatca gagcttaaag
     1741 atttctttca aagttatgga aacgtggtgg agttgcgcat taacagtggt gggaaattac
     1801 ccaattttgg ttttgttgtg tttgatgatt ctgagcctgt tcagaaagtc cttagcaaca
     1861 ggcccatcat gttcagaggt gaggtccgtc tgaatgtcga agagaagaag actcgagctg
     1921 ccagggaagg cgaccgacga gataatcgcc ttcggggacc tggaggccct cgaggtgggc
     1981 tgggtggtgg aatgagaggc cctccccgtg gaggcatggt gcagaaacca ggatttggag
     2041 tgggaagggg gcttgcgcca cggcagtgaA CCCAGCTTTC TTGTACAAAG TTGGCATTAT
     2101 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT
     2161 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT
     2221 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT
     2281 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT
     2341 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT
     2401 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT
     2461 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA
     2521 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC
     2581 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG
     2641 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC
     2701 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG
     2761 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC
     2821 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA
     2881 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA
     2941 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC
     3001 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT
     3061 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT
     3121 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA
     3181 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG
     3241 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
     3301 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
     3361 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
     3421 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
     3481 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
     3541 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
     3601 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
     3661 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
     3721 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
     3781 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
     3841 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
     3901 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT
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