LOCUS       pENTR4_G3BP2a           3995 bp    DNA     circular     09-APR-2019
DISCLAIMER  Certain terms are trademarks or registered trademarks of Invitrogen 
            Corporation. See "Intellectual Property" in the Help file for more 
            information.
FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
                     /note="recognized as attL1"
     misc_recomb     complement(2120..2218)
                     /label=attL2
                     /note="attL2 (c)"
                     /note="recognized as attL2"
     misc_signal     complement(2233..2252)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2260..2276
                     /note="M13 Reverse priming site"
     gene            2389..3198
                     /note="Kanamycin resistance gene"
     rep_origin      3319..3992
                     /note="pUC origin"
     source          671..2119
                     /dnas_title="pENTR4_G3BP2a"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q21.1"
     CDS             671..2119
                     /gene="G3BP2"
                     /note="isoform a is encoded by transcript variant 1;
                     Ras-GTPase activating protein SH3 domain-binding protein
                     2; ras GTPase-activating protein-binding protein 2;
                     G3BP-2; GAP SH3 domain-binding protein 2"
                     /codon_start=1
                     /product="ras GTPase-activating protein-binding protein 2
                     isoform a"
                     /protein_id="NP_987101.1"
                     /db_xref="GI:45359849"
                     /db_xref="CCDS:CCDS3571.1"
                     /db_xref="GeneID:9908"
                     /db_xref="HGNC:30291"
                     /translation="MVMEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGG
                     VDASGKPQEAVYGQNDIHHKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSG
                     QPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEER
                     QPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNL
                     EELEEKSTTPPPAEPVSLPQEPPKAFSWASVTSKNLPPSGTVSSSGIPPHVKAPVSQP
                     RVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLF
                     VGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSEPVQRILIAK
                     PIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRD
                     GRGPPPRGGMAQKLGSGRGTGQMEGRFTGQRR"
     insert          671..2119
                     /source="NM_203505.2"
     vector          join(2130..3995,1..658)
                     /source="pDONR%99221"
     clone           659..2129
                     /source="G3BP2a AttB Insert"
ORIGIN      
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTC atggttatgg agaagcccag tccgctgctt gtagggcggg agtttgtgag
      721 gcaatattat actttgctga ataaagctcc ggaatattta cacaggtttt atggcaggaa
      781 ttcttcctat gttcatggtg gagtagatgc tagtggaaag ccccaggaag ctgtttatgg
      841 ccaaaatgat atacaccaca aagtattatc tctgaacttc agtgaatgtc atactaaaat
      901 tcgtcatgtg gatgctcatg caaccttgag tgatggagta gttgtccagg tcatgggttt
      961 gctgtctaac agtggacaac cagaaagaaa gtttatgcaa acctttgttc tggctcctga
     1021 aggatctgtt ccaaataaat tttatgttca caatgatatg tttcgttatg aagatgaagt
     1081 gtttggtgat tctgagcctg aacttgatga agaatcagaa gatgaagtag aagaggaaca
     1141 agaagaaaga caaccatctc ctgaacctgt gcaagaaaat gctaacagtg gttactatga
     1201 agctcaccct gtgactaatg gcatagagga gcctttggaa gaatcctctc atgaacctga
     1261 acctgagcca gaatctgaaa caaagactga agagctgaaa ccacaagtgg aggagaagaa
     1321 cttagaagaa ctagaggaga aatctactac tcctcctccg gcagaacctg tttctctgcc
     1381 acaagaacca ccaaaggctt tctcctgggc ttcagtgacc agtaaaaacc tgcctcctag
     1441 tggtactgtt tcttcctctg gaattccacc ccatgttaaa gcaccagtct cacagccaag
     1501 agtcgaagct aaaccagaag ttcaatctca gccacctcgt gtgcgtgaac aacgacctag
     1561 agaacgacct ggttttcctc ctagaggacc aagaccaggc agaggagata tggaacagaa
     1621 tgactctgac aaccgtagaa taattcgcta tccagatagt catcaacttt ttgttggtaa
     1681 cttgccacat gatattgatg aaaatgagct aaaggaattc ttcatgagtt ttggaaacgt
     1741 tgtggaactt cgcatcaata ccaagggtgt tgggggaaag cttccaaatt ttggttttgt
     1801 ggtttttgat gactctgaac cagttcagag aatcttaatt gcaaaaccga ttatgtttcg
     1861 aggggaagta cgtttaaatg tggaagagaa aaaaacaaga gctgcaagag agcgagaaac
     1921 cagaggtggt ggtgatgatc gcagggatat taggcgcaat gatcgaggtc ccggtggtcc
     1981 acgtggaatt gtgggtggtg gaatgatgcg tgatcgtgat ggaagaggac ctcctccaag
     2041 gggtggcatg gcacagaaac ttggctctgg aagaggaacc gggcaaatgg agggccgctt
     2101 cacaggacag cgtcgctgaA CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT
     2161 TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC
     2221 CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG
     2281 CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA
     2341 TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT
     2401 CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT
     2461 AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG
     2521 CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA
     2581 GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT
     2641 TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA
     2701 TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG
     2761 TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA
     2821 TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT
     2881 GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG
     2941 CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT
     3001 GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC
     3061 CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG
     3121 CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG
     3181 CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG
     3241 CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG
     3301 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT
     3361 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
     3421 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
     3481 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
     3541 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
     3601 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
     3661 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
     3721 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
     3781 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC
     3841 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
     3901 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG
     3961 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT
//

