LOCUS       pFRT/TO/DESTFLAG        7027 bp    DNA     circular     19-MAR-2007
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDBDATE|441289996|
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FEATURES             Location/Qualifiers
     misc_recomb     3241..3288
                     /dnas_title="FRT"
                     /vntifkey="86"
                     /label=FRT
     CDS             3296..4316
                     /dnas_title="Hygromycin"
                     /vntifkey="4"
                     /label=Hygromycin
     misc_feature    complement(5779..6639)
                     /dnas_title="Ampicillin"
                     /vntifkey="21"
                     /label=Ampicillin
     rep_origin      complement(4961..5634)
                     /dnas_title="pUC ori"
                     /vntifkey="33"
                     /label=pUC ori
     polyA_signal    2733..2957
                     /dnas_title="BGH"
                     /vntifkey="25"
                     /label=BGH
     polyA_signal    4448..4578
                     /dnas_title="SV40 early"
                     /vntifkey="25"
                     /label=SV40 early
     promoter        274..1000
                     /dnas_title="CMV"
                     /vntifkey="29"
                     /label=CMV
     CDS             1032..1100
                     /dnas_title="FLAGHA"
                     /vntifkey="4"
                     /label=FLAGHA
     misc_recomb     1101..1125
                     /dnas_title="attB1"
                     /vntifkey="21"
                     /label=attB1
                     /note="recognized as attB1"
     misc_recomb     complement(2529..2553)
                     /dnas_title="attB2"
                     /vntifkey="21"
                     /label=attB2
                     /note="recognized as attB2"
     CDS             2606..2647
                     /dnas_title="V5"
                     /vntifkey="4"
                     /label=V5
     protein_bind    860..901
                     /note="2x TetO2 "
     vector          join(2539..6775,1..1115)
                     /source="pFRT/TO/DESTFLAGHA"
     source          1128..2528
                     /dnas_title="pFRT/TO/DESTFLAGHA"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q33.1"
     CDS             1128..2528
                     /dnas_title="G3BP1"
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /EC_number="3.6.4.13"
                     /EC_number="3.6.4.12"
                     /note="Ras-GTPase-activating protein SH3-domain-binding
                     protein; GAP binding protein; RasGAP-associated
                     endoribonuclease G3BP; G3BP-1; ATP-dependent DNA helicase
                     VIII; GAP SH3 domain-binding protein 1"
                     /codon_start=1
                     /product="ras GTPase-activating protein-binding protein 1"
                     /protein_id="NP_005745.1"
                     /db_xref="GI:5031703"
                     /db_xref="CCDS:CCDS4319.1"
                     /db_xref="GeneID:10146"
                     /db_xref="HGNC:30292"
                     /db_xref="HPRD:06569"
                     /db_xref="MIM:608431"
                     /translation="MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGG
                     LDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNN
                     QALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQ
                     QTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPV
                     LEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVK
                     VPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMV
                     RHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQ
                     KVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGM
                     VQKPGFGVGRGLAPRQ"
     insert          1128..2528
                     /source="G3BP1 AttB Insert"
ORIGIN      
        1 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cggacggatc gggagatctc
       61 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
      121 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      181 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      241 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      301 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      361 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      421 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      661 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      721 gtctccaccc cattgacgtc aatgggagtt tgttttggaa ccaaaatcaa cgggactttc
      781 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      841 aggtctatat aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
      901 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      961 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
     1021 agcttggtac catggactac aaggacgacg atgacaagta cccttatgac gtgcccgatt
     1081 acgctttgaa ccagatatca acaagtttgt acaaaAAAGC AGGCTTCatg gtgatggaga
     1141 agcctagtcc cctgctggtc gggcgggaat ttgtgagaca gtattacaca ctgctgaacc
     1201 aggccccaga catgctgcat agattttatg gaaagaactc ttcttatgtc catgggggat
     1261 tggattcaaa tggaaagcca gcagatgcag tctacggaca gaaagaaatc cacaggaaag
     1321 tgatgtcaca aaacttcacc aactgccaca ccaagattcg ccatgttgat gctcatgcca
     1381 cgctaaatga tggtgtggta gtccaggtga tggggcttct ctctaacaac aaccaggctt
     1441 tgaggagatt catgcaaacg tttgtccttg ctcctgaggg gtctgttgca aataaattct
     1501 atgttcacaa tgatatcttc agataccaag atgaggtctt tggtgggttt gtcactgagc
     1561 ctcaggagga gtctgaagaa gaagtagagg aacctgaaga aagacagcaa acacctgagg
     1621 tggtacctga tgattctgga actttctatg atcaggcagt tgtcagtaat gacatggaag
     1681 aacatttaga ggagcctgtt gctgaaccag agcctgatcc tgaaccagaa ccagaacaag
     1741 aacctgtatc tgaaatccaa gaggaaaagc ctgagccagt attagaagaa actgcccctg
     1801 aggatgctca gaagagttct tctccagcac ctgcagacat agctcagaca gtacaggaag
     1861 acttgaggac attttcttgg gcatctgtga ccagtaagaa tcttccaccc agtggagctg
     1921 ttccagttac tgggatacca cctcatgttg ttaaagtacc agcttcacag ccccgtccag
     1981 agtctaagcc tgaatctcag attccaccac aaagacctca gcgggatcaa agagtgcgag
     2041 aacaacgaat aaatattcct ccccaaaggg gacccagacc aatccgtgag gctggtgagc
     2101 aaggtgacat tgaaccccga agaatggtga gacaccctga cagtcaccaa ctcttcattg
     2161 gcaacctgcc tcatgaagtg gacaaatcag agcttaaaga tttctttcaa agttatggaa
     2221 acgtggtgga gttgcgcatt aacagtggtg ggaaattacc caattttggt tttgttgtgt
     2281 ttgatgattc tgagcctgtt cagaaagtcc ttagcaacag gcccatcatg ttcagaggtg
     2341 aggtccgtct gaatgtcgaa gagaagaaga ctcgagctgc cagggaaggc gaccgacgag
     2401 ataatcgcct tcggggacct ggaggccctc gaggtgggct gggtggtgga atgagaggcc
     2461 ctccccgtgg aggcatggtg cagaaaccag gatttggagt gggaaggggg cttgcgccac
     2521 ggcagtgaAC CCAGCTTTct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc
     2581 aagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     2641 ttctacgcgt accggatccg cggccgcata gataactgat ccagtgtgcc tcgagtctag
     2701 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     2761 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     2821 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     2881 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     2941 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     3001 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3061 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta
     3241 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa
     3301 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     3361 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     3421 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     3481 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     3541 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     3601 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     3661 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     3721 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     3781 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     3841 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     3901 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     3961 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     4021 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     4081 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     4141 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     4201 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     4261 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca
     4321 cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     4381 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     4441 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4501 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4561 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4621 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4681 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4741 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4801 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4861 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4921 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4981 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5041 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5101 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5161 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5221 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5281 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5341 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5401 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5461 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5521 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5581 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5641 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5701 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6361 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//

